Halomonas Maris Sp. Nov., a Moderately Halophilic Bacterium Isolated From Marine Sediment in the Southwest Indian Ocean
A halophilic, Gram-staining-negative, rod-shaped, flagellated and motile bacterium, strain QX-1T, was isolated from deep-sea sediment at a depth of 3332 m in the southwestern Indian Ocean. Strain QX-1T growth was observed at 4–50 °C (optimum 37 °C), pH 5.0–11.0 (optimum pH 7.0), 3%–25% NaCl (w/v; optimum 7%), and it did not grow without NaCl. A phylogenetic analysis based on the 16S rRNA gene placed strain QX-1T in the genus Halomonas and most closely related to Halomonas sulfidaeris (97.90%), Halomonas zhaodongensis (97.80%), Halomonas songnenensis (97.59%), Halomonas hydrothermalis (97.37%), Halomonas subterranea (97.25%), Halomonas salicampi (97.09%), and Halomonas arcis (97.01%). DNA–DNA hybridization (< 26.50%) and average nucleotide identity values (< 83.54%) between strain QX-1T and the related type strains meet the accepted criteria for a new species. The principal fatty acids (> 10%) of strain QX-1T are C16:0 (25.50%), C17:0 cyclo (14.02%), C19:0 cyclo ω8c (18.72%), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, 18.08%). The polar lipids of strain QX-1T are mainly diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified aminophospholipid, and five unidentified lipids. The main respiratory quinone is Q-9. The G+C content of its chromosomal DNA is 54.4 mol%. Its fatty acid profile, respiratory quinones, and G+C content also support the placement of QX-1T in the genus Halomonas. These phylogenetic, phenotypic, and chemotaxonomic analyses indicate that QX-1T is a novel species, for which the name Halomonas maris is proposed. The type strain is QX-1T (=MCCC 1A17875T = KCTC 82198T = NBRC 114670T).
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Posted 22 Feb, 2021
Received 09 Feb, 2021
Invitations sent on 07 Feb, 2021
On 05 Feb, 2021
On 03 Feb, 2021
Halomonas Maris Sp. Nov., a Moderately Halophilic Bacterium Isolated From Marine Sediment in the Southwest Indian Ocean
Posted 22 Feb, 2021
Received 09 Feb, 2021
Invitations sent on 07 Feb, 2021
On 05 Feb, 2021
On 03 Feb, 2021
A halophilic, Gram-staining-negative, rod-shaped, flagellated and motile bacterium, strain QX-1T, was isolated from deep-sea sediment at a depth of 3332 m in the southwestern Indian Ocean. Strain QX-1T growth was observed at 4–50 °C (optimum 37 °C), pH 5.0–11.0 (optimum pH 7.0), 3%–25% NaCl (w/v; optimum 7%), and it did not grow without NaCl. A phylogenetic analysis based on the 16S rRNA gene placed strain QX-1T in the genus Halomonas and most closely related to Halomonas sulfidaeris (97.90%), Halomonas zhaodongensis (97.80%), Halomonas songnenensis (97.59%), Halomonas hydrothermalis (97.37%), Halomonas subterranea (97.25%), Halomonas salicampi (97.09%), and Halomonas arcis (97.01%). DNA–DNA hybridization (< 26.50%) and average nucleotide identity values (< 83.54%) between strain QX-1T and the related type strains meet the accepted criteria for a new species. The principal fatty acids (> 10%) of strain QX-1T are C16:0 (25.50%), C17:0 cyclo (14.02%), C19:0 cyclo ω8c (18.72%), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, 18.08%). The polar lipids of strain QX-1T are mainly diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified aminophospholipid, and five unidentified lipids. The main respiratory quinone is Q-9. The G+C content of its chromosomal DNA is 54.4 mol%. Its fatty acid profile, respiratory quinones, and G+C content also support the placement of QX-1T in the genus Halomonas. These phylogenetic, phenotypic, and chemotaxonomic analyses indicate that QX-1T is a novel species, for which the name Halomonas maris is proposed. The type strain is QX-1T (=MCCC 1A17875T = KCTC 82198T = NBRC 114670T).
Figure 1