Objective: Atherosclerosis (AS) is the main pathological basis of most cardiovascular diseases, numerous studies have shown that programmed cell death and lncRNA-miRNA-mRNA regulatory network play an important role in cardiovascular diseases. However, the crosstalk between the ceRNA network associated with programmed cell death and AS remains unclear. This article aims to explore the role of programmed cell death-related ceRNA network in the pathophysiological process of AS, in order to provide new targets for the diagnosis and treatment of AS.:
Methods: Firstly, the GSE97210 and GSE28858 datasets were screened from the GEO database, and the differentially expressed lncRNA, mRNA and miRNA were screened, and then the lncRNA-miRNA-mRNA regulatory network was constructed in Cytoscape 3.7.2 software based on ceRNA theory. Second, GO and KEGG enrichment analysis of mRNA in the ceRNA network was performed. Finally, the mRNAs in the ceRNA network were intersected with genes related to autophagy, pyroptosis and ferroptosis to construct a ceRNA network related to programmed cell death:
Results: According to the results of bioinformatics analysis, a total of 1208 DElncRNAs, 4723 DEmRNAs and 139 DEmiRNAs were obtained. A ceRNA network consisting of 64 lncRNAs, 8 miRNAs and 167 mRNAs was constructed. The mRNAs in the CeRNA network are mainly enriched in biological functions such as positive regulation of transcription and migration, protein binding, GTPase activity, and signaling pathways such as PI3K-Akt signaling pathway, ErbB signaling pathway, and mTOR signaling pathway. Validated by receiver operating characteristic (ROC) curves, 7 lncRNA-mediated ceRNA regulatory pathways for pyroptosis and 23 lncRNA-mediated regulatory pathways for ferroptosis and autophagy were constructed.:
Conclusion: This study screened and identified the lncRNA, miRNA and mRNA expression profiles specifically expressed in AS tissues based on bioinformatics analysis, and constructed a ceRNA network related to programmed cell death, which is helpful for us to understand that programmed cell death leads to AS The mechanism of action, while providing new insights into programmed cell death in AS.: