This preprint is under consideration at Annals of General Psychiatry. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Primary research
Soundravally Rajendiran
JIPMER
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Two common variants of Methylene tetrahydrofolate reductase (MTHFR) are C677T and A1298C, which have been associated with general depression. Main objective of the study was to explore how these functional polymorphisms of MTHFR gene associate with the risk to develop postpartum depression.
Method: 434 women were recruited in a study period of 3 years (January 2014 to December 2017) in a tertiary care centre. EPDS was administered at 6 weeks postpartum, with a cut-off score of ≥10 to define probable postpartum depression. Plasma was used for the estimation of circulating vitamin B12 metabolites and whole blood for extraction of DNA. Real time PCR using Taqman probes was used for genotyping the proposed SNP’s.
Results: T allele of MTHFR C677T was associated with the risk of probable PPD (p = 0.024, OR = 1.67; 95% CI: 1.06-2.62) and presence of suicidal ideations in these women (p<0.001, OR = 2.90; 95% CI: 1.61-5.24). Haplotype 677T-1298A was associated with both the risk of probable PPD (p = 0.029, OR = 1.66; 95% CI: 1.05-2.64) and suicidal ideations (p <0.001, OR = 2.933; 95% CI: 1.60-5.35), whereas 677C-1298A was protective against suicidal ideations (p = 0.011; OR = 0.558; 95% CI: 0.35-0.88). Women with risk allele T were also associated with higher levels of circulating holotranscobalamin levels, and lower levels of vitamin B12, whereas risk allele C of A1298C had lower levels of holotranscobalamin.
Limitations: Cross-sectional study
Conclusion: Polymorphic variants of MTHFR gene might indicate susceptibility to develop probable depression in postpartum period.
Significance
MTHFR C677T has been widely reported in the literature to be associated with general depression. The association of this variant in susceptibility to develop postpartum depression is not widely studied. We observed T allele of MTHFR C677T to be associated with the risk of to develop depression and suicidal ideation in postpartum period. Higher levels of MMA and holotranscobalamin, and lower levels of vitamin B12 were associated with T allele of MTHFR C677T. Polymorphic variants of MTHFR gene might indicate susceptibility to develop depression in postpartum period.
Keywords
Depression, postpartum, folate
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This preprint is under consideration at Annals of General Psychiatry. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Primary research
Soundravally Rajendiran
JIPMER
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 09 Mar, 2021
No community comments so far
Submission checks complete
On 06 Feb, 2021
Editor invited
On 06 Feb, 2021
First submitted
On 04 Feb, 2021
Editor assigned
On 04 Feb, 2021
Subject Areas
Psychiatry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Two common variants of Methylene tetrahydrofolate reductase (MTHFR) are C677T and A1298C, which have been associated with general depression. Main objective of the study was to explore how these functional polymorphisms of MTHFR gene associate with the risk to develop postpartum depression.
Method: 434 women were recruited in a study period of 3 years (January 2014 to December 2017) in a tertiary care centre. EPDS was administered at 6 weeks postpartum, with a cut-off score of ≥10 to define probable postpartum depression. Plasma was used for the estimation of circulating vitamin B12 metabolites and whole blood for extraction of DNA. Real time PCR using Taqman probes was used for genotyping the proposed SNP’s.
Results: T allele of MTHFR C677T was associated with the risk of probable PPD (p = 0.024, OR = 1.67; 95% CI: 1.06-2.62) and presence of suicidal ideations in these women (p<0.001, OR = 2.90; 95% CI: 1.61-5.24). Haplotype 677T-1298A was associated with both the risk of probable PPD (p = 0.029, OR = 1.66; 95% CI: 1.05-2.64) and suicidal ideations (p <0.001, OR = 2.933; 95% CI: 1.60-5.35), whereas 677C-1298A was protective against suicidal ideations (p = 0.011; OR = 0.558; 95% CI: 0.35-0.88). Women with risk allele T were also associated with higher levels of circulating holotranscobalamin levels, and lower levels of vitamin B12, whereas risk allele C of A1298C had lower levels of holotranscobalamin.
Limitations: Cross-sectional study
Conclusion: Polymorphic variants of MTHFR gene might indicate susceptibility to develop probable depression in postpartum period.
Significance
MTHFR C677T has been widely reported in the literature to be associated with general depression. The association of this variant in susceptibility to develop postpartum depression is not widely studied. We observed T allele of MTHFR C677T to be associated with the risk of to develop depression and suicidal ideation in postpartum period. Higher levels of MMA and holotranscobalamin, and lower levels of vitamin B12 were associated with T allele of MTHFR C677T. Polymorphic variants of MTHFR gene might indicate susceptibility to develop depression in postpartum period.
Figure 1
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