3.1 Epidemiological history
39 patients lived in Wuhan, 21 of them had close contact history (53.8%), 6 had been to the area where people gathered, 12 had no special epidemiological history. The main symptoms of the patients were fever: 34 cases (87.2%, less than 38.5 degrees 26 cases, > 38.5 degrees 8 cases), cough in 27 cases (69.2%, dry cough in 15 cases, cough in foam like sputum 12 cases), chest tightness 26 cases (66.7%), fatigue 36 cases (92.3%), sore throat 30 cases (76.9%), diarrhoea 5 cases (12.8%), muscle soreness 11 cases(28.2%).
3.2 CT manifestations
The patients were examined every week after the onset of the disease: < 7 days, 7-14 days, 14 days -discharge, and 2 weeks after discharge.
(1) Range of lesion involvement
< 7 days: the lesions were located in bilateral lungs in 12 cases, unilateral lung in 27 cases (left lung in 11 cases, right lung in 16 cases);
7-14 days: the lesions were located in bilateral lungs in 26 cases, unilateral lung in 13 cases (left lung in 5 cases, right lung in 8 cases);
14 days - discharge: the lesions were located in bilateral lungs in 25 cases, unilateral lung in 11 cases (left lung in 4 cases, right lung in 7 cases); 3 cases without lesions in lung;
Two weeks after discharge: the lesions were located in bilateral lungs in 13 cases, unilateral lung in 6 cases (left lung in 2 cases, right lung in 4 cases); 20 cases without lesions in lung;
(2) Distribution and morphological characteristics of lesions:Calculate the number of lung segments with lesions.
There are three types of lesions: ground glass opacity lesions (including ground glass nodule and flake ground glass shadow), solid lesions (including solid nodule and flake shadow), and strip like lesions. The types of lesions in the involved lung segments were counted. If multiple lesions existed at the one segment, they were calculated separately.
< 7 days: 86 lung segments were involved. There were 79 ground glass opacity lesions(Fig.1), 26 solid lesions and 17 strip like lesions.
7-14 days: 214 lung segments were involved. There were 195 ground glass opacity lesions(Fig.2), 141 solid lesions and 108 strip like lesions.
14 days - discharge: 113 lung segments were involved. There were 64 ground glass opacity lesions, 47 solid lesions and 93 strip like lesions(Fig.3). The density of most ground glass lesions decreased significantly.
Two weeks after discharge: 39 lung segments were involved (2 patients had new lesions in 3 lung segments). There were 79 ground glass opacity lesions (3 new lesions), 26 solid lesions and 17 strip like lesions(Fig.4).
3.3 Laboratory examination
Blood routine examination on admission: Lymphocyte was normal in 11 cases, Lymphocyte decreased in 28 cases.
C-reactive protein on admission: Increased significantly in 32 cases, no increase in 7 cases. The reference value of our hospital is < 8mg/L.
During 7-14 days of onset, D-dimer was normal in 19 cases, increased in 12 cases (< 10 times of reference value), and significantly increased in 8 cases (> 10 times of reference value). Note: the reference value of our hospital is < 0.5mg/L.
3.4 Detection of nucleic acid in nasopharynx swab by RT-PCR
Contains nCovORF lab and 2019nCov-N. Note: the reference value of our hospital is < 10Au/ml. RT-PCR results of 39 patients at admission: 26 cases were positive for both nCovORF lab and 2019nCov-N, 5 cases were positive only for nCovORF lab,8 cases were positive only for 2019nCov-N.
RT-PCR was negative at the time of discharge, and the interval between the two tests was at least one day.
Two weeks after discharge, RT-PCR was positive in 2 patients with new CT lesions. One case was nCovORF lab(+) and 2019nCov-N(+). One case was nCovORF lab(-) and 2019nCov-N(+).The second patient experienced RT-PCR tests two times, the first time was negative, the second time was positive, the interval was 3 days.
3.5 Detection of virus antibody
At the time of discharge, the patients were tested for virus antibody, including 2019N Cov antibody IgM and 2019N Cov antibody IgG. Among them, 15 cases were IgM (+) IgG (+),21 cases were IgM (-) IgG (+),3 cases were IgM (-) IgG (-).
Reexamination 2 weeks after discharge,two patients with positive nucleic acid test,the antibody test results of these two patients at discharge: one case was IgM (+)IgG(+), the other case was IgM (-)IgG(+).