Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections
Background: Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown.
Methods: To study the mechanisms responsible for resistance elicited by IL-25, we analyze the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compare the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni -infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae.
Results: Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Forty of those spots validated protein spots were identified by mass spectrometry corresponding to 24 proteins.
Conclusions: The analysis of our results indicates that resistance to infectiuon is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance.
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Posted 20 May, 2020
On 02 Jul, 2020
On 25 May, 2020
Received 24 May, 2020
On 11 May, 2020
Received 09 May, 2020
Received 09 May, 2020
On 08 May, 2020
On 08 May, 2020
Invitations sent on 08 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
Received 04 May, 2020
On 04 May, 2020
Received 03 May, 2020
Received 27 Apr, 2020
On 26 Apr, 2020
On 25 Apr, 2020
On 24 Apr, 2020
Invitations sent on 18 Apr, 2020
On 17 Apr, 2020
On 16 Apr, 2020
On 16 Apr, 2020
On 10 Apr, 2020
On 07 Apr, 2020
On 07 Apr, 2020
On 06 Apr, 2020
On 06 Apr, 2020
Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections
Posted 20 May, 2020
On 02 Jul, 2020
On 25 May, 2020
Received 24 May, 2020
On 11 May, 2020
Received 09 May, 2020
Received 09 May, 2020
On 08 May, 2020
On 08 May, 2020
Invitations sent on 08 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
Received 04 May, 2020
On 04 May, 2020
Received 03 May, 2020
Received 27 Apr, 2020
On 26 Apr, 2020
On 25 Apr, 2020
On 24 Apr, 2020
Invitations sent on 18 Apr, 2020
On 17 Apr, 2020
On 16 Apr, 2020
On 16 Apr, 2020
On 10 Apr, 2020
On 07 Apr, 2020
On 07 Apr, 2020
On 06 Apr, 2020
On 06 Apr, 2020
Background: Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown.
Methods: To study the mechanisms responsible for resistance elicited by IL-25, we analyze the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compare the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni -infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae.
Results: Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Forty of those spots validated protein spots were identified by mass spectrometry corresponding to 24 proteins.
Conclusions: The analysis of our results indicates that resistance to infectiuon is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance.
Figure 1
Figure 2
Figure 3
Figure 4