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Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections
Rafael Toledo
Universitat de Valencia
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2651-9794
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Parasites & Vectors.
Background: Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown.
Methods: To study the mechanisms responsible for resistance elicited by IL-25, we analyze the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compare the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni -infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae.
Results: Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Forty of those spots validated protein spots were identified by mass spectrometry corresponding to 24 proteins.
Conclusions: The analysis of our results indicates that resistance to infectiuon is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance.
Keywords
proteomics, intestine, interleukin-25, intestinal helminths, trematoda, Echinostoma caproni
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On 08 May, 2020
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On 08 May, 2020
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Invitations sent on 08 May, 2020
Editor assigned
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On 06 May, 2020
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Received 04 May, 2020
Editorial decision: Major Revision
On 04 May, 2020
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Received 03 May, 2020
Review #1 received
Received 27 Apr, 2020
Reviewer #3 agreed
On 26 Apr, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 24 Apr, 2020
Reviewers invited
Invitations sent on 18 Apr, 2020
Editor assigned
On 17 Apr, 2020
Submission checks complete
On 16 Apr, 2020
Editor invited
On 16 Apr, 2020
No comments provided
Editorial decision: Revise before peer review
On 10 Apr, 2020
Editor invited
On 07 Apr, 2020
Editor assigned
On 07 Apr, 2020
First submitted
On 06 Apr, 2020
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On 06 Apr, 2020
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Subject Areas
Parasitology
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This preprint is under consideration at Parasites & Vectors. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections
Rafael Toledo
Universitat de Valencia
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2651-9794
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 20 May, 2020
Published
On 02 Jul, 2020
No community comments so far
Editorial decision: Minor Revision
On 25 May, 2020
Review #3 received
Received 24 May, 2020
Reviewer #3 agreed
On 11 May, 2020
Review #1 received
Received 09 May, 2020
Review #2 received
Received 09 May, 2020
Reviewer #1 agreed
On 08 May, 2020
Reviewer #2 agreed
On 08 May, 2020
Reviewers invited
Invitations sent on 08 May, 2020
Editor assigned
On 07 May, 2020
Submission checks complete
On 06 May, 2020
Editor invited
On 06 May, 2020
No comments provided
Review #2 received
Received 04 May, 2020
Editorial decision: Major Revision
On 04 May, 2020
Review #3 received
Received 03 May, 2020
Review #1 received
Received 27 Apr, 2020
Reviewer #3 agreed
On 26 Apr, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 24 Apr, 2020
Reviewers invited
Invitations sent on 18 Apr, 2020
Editor assigned
On 17 Apr, 2020
Submission checks complete
On 16 Apr, 2020
Editor invited
On 16 Apr, 2020
No comments provided
Editorial decision: Revise before peer review
On 10 Apr, 2020
Editor invited
On 07 Apr, 2020
Editor assigned
On 07 Apr, 2020
First submitted
On 06 Apr, 2020
Submission checks complete
On 06 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Parasites & Vectors.
Background: Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown.
Methods: To study the mechanisms responsible for resistance elicited by IL-25, we analyze the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compare the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni -infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae.
Results: Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Forty of those spots validated protein spots were identified by mass spectrometry corresponding to 24 proteins.
Conclusions: The analysis of our results indicates that resistance to infectiuon is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance.
Figure 1
Figure 2
Figure 3
Figure 4