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NTCP gene polymorphisms and Hepatitis B virus infection status in a Ghanaian population
Christian Obirikorang
Kwame Nkrumah University of Science and Technology College of Health Sciences
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Virology Journal.
Background: SLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population.
Method: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects.
Results: The minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p<0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3% and 8.2% among the control and the case group, respectively (p =0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR= 0.18 (0.07-0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR= 5.2 (95%CI: 2.1 -12.8); 3.5 (95%CI: 1.6 -7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p >0.05).
Conclusion: The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.
Keywords
SLC10A1, NTCP, SNP, CHB infection, PCR-RFLP.
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CURRENT STATUS: Published
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Posted 19 Jun, 2020
Published
On 03 Jul, 2020
No community comments so far
Reviewer #2 agreed
On 27 Jun, 2020
Review #2 received
Received 27 Jun, 2020
Editorial decision: Accept
On 27 Jun, 2020
Reviewer #1 agreed
On 19 Jun, 2020
Review #1 received
Received 19 Jun, 2020
Editor assigned
On 17 Jun, 2020
Reviewers invited
Invitations sent on 17 Jun, 2020
Submission checks complete
On 16 Jun, 2020
Editor invited
On 16 Jun, 2020
No comments provided
Review #1 received
Received 14 May, 2020
Review #2 received
Received 14 May, 2020
Editorial decision: Major revision
On 14 May, 2020
Reviewer #2 agreed
On 01 May, 2020
Reviewer #1 agreed
On 30 Apr, 2020
Reviewers invited
Invitations sent on 29 Apr, 2020
Editor assigned
On 27 Apr, 2020
Editor invited
On 26 Apr, 2020
Submission checks complete
On 22 Apr, 2020
First submitted
On 16 Apr, 2020
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Subject Areas
Virology
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This preprint is under consideration at Virology Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
NTCP gene polymorphisms and Hepatitis B virus infection status in a Ghanaian population
Christian Obirikorang
Kwame Nkrumah University of Science and Technology College of Health Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 19 Jun, 2020
Published
On 03 Jul, 2020
No community comments so far
Reviewer #2 agreed
On 27 Jun, 2020
Review #2 received
Received 27 Jun, 2020
Editorial decision: Accept
On 27 Jun, 2020
Reviewer #1 agreed
On 19 Jun, 2020
Review #1 received
Received 19 Jun, 2020
Editor assigned
On 17 Jun, 2020
Reviewers invited
Invitations sent on 17 Jun, 2020
Submission checks complete
On 16 Jun, 2020
Editor invited
On 16 Jun, 2020
No comments provided
Review #1 received
Received 14 May, 2020
Review #2 received
Received 14 May, 2020
Editorial decision: Major revision
On 14 May, 2020
Reviewer #2 agreed
On 01 May, 2020
Reviewer #1 agreed
On 30 Apr, 2020
Reviewers invited
Invitations sent on 29 Apr, 2020
Editor assigned
On 27 Apr, 2020
Editor invited
On 26 Apr, 2020
Submission checks complete
On 22 Apr, 2020
First submitted
On 16 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Virology Journal.
Background: SLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population.
Method: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects.
Results: The minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p<0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3% and 8.2% among the control and the case group, respectively (p =0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR= 0.18 (0.07-0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR= 5.2 (95%CI: 2.1 -12.8); 3.5 (95%CI: 1.6 -7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p >0.05).
Conclusion: The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.