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Research article
Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture
Sun-Woong Kang
Korea Instityte of Toxicology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8862-2151
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: We investigated whether electrical stimulation via indium tin oxide (ITO) could enhance the in vitro culture of neonatal rat ventricular myocytes (NRVMs), which are important in vitro models for studying the mechanisms underlying many aspects of cardiology.
Methods: Cardiomyocytes were obtained from 1-day-old neonatal rat heart ventricles. To evaluate function of NRVMs cultured on ITO with electrical stimulation, the cell viability, change of cell morphology, immunochemistry using cardiac-specific antibodies, and gene expression were tested.
Results: Defined sarcomeric structure, cell enlargement, and increased distribution of NRVMs appeared in the presence of electrical stimulation. These characteristics were absent in NRVMs cultured under standard culture conditions. In addition, the expression levels of cardiomyocyte-specific and ion channel markers were higher in NRVMs seeded on ITO-coated dishes than in the control group at 14 days after seeding. ITO-coated dishes could effectively provide electrical cues to support the in vitro culture of NRVMs.
Conclusions: These results provide supporting evidence that electrical stimulation via ITO can be effectively used to maintain culture and enhance function of cardiomyocytes in vitro.
Keywords
Indium tin oxide, Electrical stimulation, Neonatal rat ventricular myocytes, Stem cells
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Received 26 Apr, 2020
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On 13 Apr, 2020
Reviewers invited
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On 13 Apr, 2020
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On 12 Apr, 2020
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This preprint is under consideration at Biomaterials Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture
Sun-Woong Kang
Korea Instityte of Toxicology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8862-2151
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 19 May, 2020
No community comments so far
Editor assigned
On 07 May, 2020
Submission checks complete
On 06 May, 2020
Editor invited
On 06 May, 2020
No comments provided
Editorial decision: Minor revision
On 28 Apr, 2020
Review #3 received
Received 26 Apr, 2020
Review #1 received
Received 26 Apr, 2020
Review #2 received
Received 26 Apr, 2020
Reviewer #3 agreed
On 14 Apr, 2020
Editor assigned
On 13 Apr, 2020
Reviewers invited
Invitations sent on 13 Apr, 2020
Reviewer #1 agreed
On 13 Apr, 2020
Reviewer #2 agreed
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
First submitted
On 08 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biomaterials
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: We investigated whether electrical stimulation via indium tin oxide (ITO) could enhance the in vitro culture of neonatal rat ventricular myocytes (NRVMs), which are important in vitro models for studying the mechanisms underlying many aspects of cardiology.
Methods: Cardiomyocytes were obtained from 1-day-old neonatal rat heart ventricles. To evaluate function of NRVMs cultured on ITO with electrical stimulation, the cell viability, change of cell morphology, immunochemistry using cardiac-specific antibodies, and gene expression were tested.
Results: Defined sarcomeric structure, cell enlargement, and increased distribution of NRVMs appeared in the presence of electrical stimulation. These characteristics were absent in NRVMs cultured under standard culture conditions. In addition, the expression levels of cardiomyocyte-specific and ion channel markers were higher in NRVMs seeded on ITO-coated dishes than in the control group at 14 days after seeding. ITO-coated dishes could effectively provide electrical cues to support the in vitro culture of NRVMs.
Conclusions: These results provide supporting evidence that electrical stimulation via ITO can be effectively used to maintain culture and enhance function of cardiomyocytes in vitro.
Figure 1
Figure 2
Figure 3
Figure 4