Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture
Background: We investigated whether electrical stimulation via indium tin oxide (ITO) could enhance the in vitro culture of neonatal rat ventricular myocytes (NRVMs), which are important in vitro models for studying the mechanisms underlying many aspects of cardiology.
Methods: Cardiomyocytes were obtained from 1-day-old neonatal rat heart ventricles. To evaluate function of NRVMs cultured on ITO with electrical stimulation, the cell viability, change of cell morphology, immunochemistry using cardiac-specific antibodies, and gene expression were tested.
Results: Defined sarcomeric structure, cell enlargement, and increased distribution of NRVMs appeared in the presence of electrical stimulation. These characteristics were absent in NRVMs cultured under standard culture conditions. In addition, the expression levels of cardiomyocyte-specific and ion channel markers were higher in NRVMs seeded on ITO-coated dishes than in the control group at 14 days after seeding. ITO-coated dishes could effectively provide electrical cues to support the in vitro culture of NRVMs.
Conclusions: These results provide supporting evidence that electrical stimulation via ITO can be effectively used to maintain culture and enhance function of cardiomyocytes in vitro.
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Posted 19 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
On 28 Apr, 2020
Received 26 Apr, 2020
Received 26 Apr, 2020
Received 26 Apr, 2020
On 14 Apr, 2020
On 13 Apr, 2020
Invitations sent on 13 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 12 Apr, 2020
On 12 Apr, 2020
On 08 Apr, 2020
Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture
Posted 19 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
On 28 Apr, 2020
Received 26 Apr, 2020
Received 26 Apr, 2020
Received 26 Apr, 2020
On 14 Apr, 2020
On 13 Apr, 2020
Invitations sent on 13 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 12 Apr, 2020
On 12 Apr, 2020
On 08 Apr, 2020
Background: We investigated whether electrical stimulation via indium tin oxide (ITO) could enhance the in vitro culture of neonatal rat ventricular myocytes (NRVMs), which are important in vitro models for studying the mechanisms underlying many aspects of cardiology.
Methods: Cardiomyocytes were obtained from 1-day-old neonatal rat heart ventricles. To evaluate function of NRVMs cultured on ITO with electrical stimulation, the cell viability, change of cell morphology, immunochemistry using cardiac-specific antibodies, and gene expression were tested.
Results: Defined sarcomeric structure, cell enlargement, and increased distribution of NRVMs appeared in the presence of electrical stimulation. These characteristics were absent in NRVMs cultured under standard culture conditions. In addition, the expression levels of cardiomyocyte-specific and ion channel markers were higher in NRVMs seeded on ITO-coated dishes than in the control group at 14 days after seeding. ITO-coated dishes could effectively provide electrical cues to support the in vitro culture of NRVMs.
Conclusions: These results provide supporting evidence that electrical stimulation via ITO can be effectively used to maintain culture and enhance function of cardiomyocytes in vitro.
Figure 1
Figure 2
Figure 3
Figure 4