Supplementary Fig.1-1
The 23S rRNA domain V sequencing obtained from five operons of S. aureus strain KUB3961 (“3961D1-5”), which linezolid MIC of 2 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red circles. No mutations were found in any of the five ribosomal operons of strain NCTC8325.
Supplementary Fig.1-2
The 23S rRNA domain V sequencing obtained from five operons of S. aureus strain KUB3970 (“3970D1-5”), which linezolid MIC of 8 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red and green circles.
The G2576T mutation was found in ribosomal operons 4 and 5 (green circles).
Supplementary Fig.1-3
The 23S rRNA domain V sequencing obtained from five operons of S. aureus strain KUB3971 (“3971D1-5”), which Linezolid MIC of 8 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red and green circles.
The G2576T mutation was found in ribosomal operons 4 and 5 (green circles).
Supplementary Fig.1-4
The 23S rRNA domain V Sequencing obtained from five operons of S. aureus strain KUB3972 (“3972D1-5”), which linezolid MIC of >16 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red and green circles.
The G2576T mutation was found in ribosomal operons 4 and 5 (green circles).
Supplementary Fig.1-5
The 23S rRNA domain V sequencing obtained from five operons of S. aureus strain KUB3974 (“3974D1-5”), which linezolid MIC of >16 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red and green circles.
The G2576T mutation was found in ribosomal operons 1, 3, 4, and 5 (green circles).
Supplementary Fig.1-6
The 23S rRNA domain V sequencing obtained from five operons of S. aureus strain KUB3976 (“3976D1-5”), which linezolid MIC of >16 µg/mL, and linezolid-susceptible S. aureus strain NCTC8325. The nucleotide sequence numbers shown in this figure are S. aureus 23S rRNA gene numbers, which are the E. coli 23Sr RNA gene numbers plus 27, respectively. Point mutations have been reported at the positions of the nucleotide sequences indicated by red and green circles.
The G2576T mutation was found in ribosomal operons 1, 3, 4, and 5 (green circles).