Preparation of Folic Acid-conjugated Albumin Nanoparticles Containing Paclitaxel using High-pressure Homogenisation Coagulation Method

doi:10.21203/rs.3.rs-221888/v1

Download PDF

Nano Express

Preparation of Folic Acid-conjugated Albumin Nanoparticles Containing Paclitaxel using High-pressure Homogenisation Coagulation Method

https://doi.org/10.21203/rs.3.rs-221888/v1

This work is licensed under a CC BY 4.0 License

Version 1

posted

You are reading this latest preprint version

In this study, we prepared and evaluated folic acid-conjugated albumin-paclitaxel (FA-BSA-PTX) nanoparticles using a new green technique, called the high-pressure homogenisation coagulation method (HPHCM). The effect of process parameters such as BSA concentration, coagulant concentration, homogenisation time, homogenisation pressure, water/ethanol ratio, and BSA/PTX ratio was analysed to optimise nanoparticle size, albumin conversion rate, and encapsulation efficiency. BSA concentration was found to exert a great influence on albumin conversion rate and particle size. Meanwhile, the BSA/PTX ratio significantly affected the nanoparticle encapsulation efficiency. Electron microscopy showed that the freeze-dried particles mostly existed in the form of dimers and trimers with an average particle size of 300–400 nm. Infrared spectroscopy indicated that PTX was well encapsulated in BSA. Raman spectra of the synthesised nanoparticles indicated changes in the disulphide bond configuration and protein structure. In vitro drug-release analysis showed that crosslinked nanoparticles exhibited a sustained release. Furthermore, in vitro cell-uptake studies on HeLa cells showed that FA can be used as a targeting ligand for albumin carriers to enhance the active targeting effect of the nanoparticles with a high FR expression. These results suggest that HPHCM is an effective method to prepare FA-BSA-PTX drug-delivery systems.

Nanoscience

Scientific Communication

Albumin nanoparticles

Paclitaxel

Folic acid

High pressure homogenization

Cell uptake

Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the latest manuscript can be downloaded and

accessed as a PDF.

Table 1

Experimental conditions for single factor studies
Influence Factors	Level	Other Conditions
BSA Concentration (mg/mL)	5 10 20 30 40	MgCl₂ concentration 0.5 mg/mL, the volume ratio of water and ethanol 30, homogeneous pressure 600 bar, homogeneous time 3 min, mass ratio of BSA and PTX 25
MgCl₂ Concentration (mg/mL)	0.5 0.75 1 1.25 1.5	BSA concentration 10 mg/mL, the volume ratio of water and ethanol 30, homogeneous pressure 600 bar, homogeneous time 3 min, mass ratio of BSA and PTX 25
The Volume Ratio of Water and Ethanol	30 25 20 15 10	BSA concentration 10 mg/mL, MgCl₂ concentration 0.5 mg/mL, homogeneous pressure 600 bar, homogeneous time 3 min, mass ratio of BSA and PTX 25
Homogeneous Pressure (bar)	600 700 800 900 1000	BSA concentration 10 mg/mL, MgCl₂ concentration 0.5 mg/mL, the volume ratio of water and ethanol 20, homogeneous time 3 min, mass ratio of BSA and PTX 25
Homogeneous Time (min)	3 6 9 12 15	BSA Concentration 10 mg/mL, MgCl₂ concentration 0.5 mg/mL, the volume ratio of water and ethanol 20, homogeneous pressure 800 bar, mass ratio of BSA and PTX 25
Mass Ratio of BSA and PTX	25 20 15 10 5	BSA concentration 10 mg/mL, MgCl₂ concentration 0.5 mg/mL, the volume ratio of water and ethanol 20, homogeneous pressure 800 bar, homogeneous time 3 min

Download PDF

Version 1

posted

You are reading this latest preprint version

Preparation of Folic Acid-conjugated Albumin Nanoparticles Containing Paclitaxel using High-pressure Homogenisation Coagulation Method

Status:

Version 1

Abstract

Figures

Full Text

Table

Status:

Version 1