Recent studies show the significant effect of the third dimension and flow unsteadiness of laterally confined Rayleigh-Bénard convection (RBC). However, there are limited studies investigating 4D flow properties. The application of 4D two-colour laser-induced fluorescence to explore a laterally confined RBC at high Rayleigh number of Ra = 9.9 × 107 and Prandtl number of Pr = 6.1 is investigated using a scanning laser system. A two-colour, two dyes approach was employed to resolve the laser sheet intensity variations due to refractive index variations caused mainly by the generation of thermal plumes. Two temperature-sensitive fluorescent dyes with opposite sensitivities were used to enhance the overall temperature sensitivity to 7.3 % /℃. Details of the experimental procedure and optical system employed to reach such a high sensitivity is demonstrated. From the whole field temperature distribution, the dimensionless heat transfer coefficient, the Nusselt number, and its evolution was calculated for both hot and cold boundaries. Temperature field and Nusselt number obtained from 3D and 2D fields are reported to compare the results for these two scenarios. Thermal plumes were found to have a conical shape in the laterally confined RBC compared to the conventional mushroom shape. From visualization of the time-resolved 3D temperature field and 2D distribution of the Nusselt number, it was also found that only by volumetric measurement, temporal and spatial variations of the temperature and heat transfer can be evaluated. This shows that to evaluate the classic and ultimate theories, volumetric measurement is required for a coherent understanding of the physics.