Open Source Board Based Acoustofluidic Transwells for Reversible Disruption of the Blood-Brain Barrier for Therapeutic Delivery

doi:10.21203/rs.3.rs-2323098/v1

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Research Article

Open Source Board Based Acoustofluidic Transwells for Reversible Disruption of the Blood-Brain Barrier for Therapeutic Delivery

https://doi.org/10.21203/rs.3.rs-2323098/v1

This work is licensed under a CC BY 4.0 License

Journal Publication

published 14 Jul, 2023

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Background

Blood-brain barrier (BBB) is a crucial but dynamic structure that functions as a gatekeeper for the central nervous system (CNS). Managing sufficient substances across the BBB is a major challenge, especially in the development of therapeutics for CNS disorders.

Methods

To achieve an efficient, fast and safe strategy for BBB opening, an acoustofluidic transwell (AFT) was developed for reversible disruption of the BBB. The proposed AFT was consisted of a transwell insert where the BBB model was established, and a surface acoustic wave (SAW) transducer realized using open-source electronics based on printed circuit board techniques.

Results

In the AFT device, the SAW produced acousto-mechanical stimulations to the BBB model resulting in decreased transendothelial electrical resistance in a dose dependent manner, indicating the disruption of the BBB. Moreover, SAW stimulation enhanced transendothelial permeability to sodium fluorescein and FITC-dextran with various molecular weight in the AFT device. Further study indicated BBB opening was attributed to the apparent stretching of intercellular spaces. An in vivo study using a zebrafish model demonstrated SAW exposure promoted penetration of sodium fluorescein to the CNS.

Conclusions

In summary, AFT effectively disrupts the BBB under the SAW stimulation, which is promising as a new drug delivery methodology for neurodegenerative diseases.

acoustofluidic transwell

blood-brain barrier

surface acoustic wave

human brain microvascular endothelial cells

transendothelial electrical resistance

The blood-brain barrier (BBB) is a multicellular vascular structure that separates the blood from the central nervous system (CNS) and regulates the delivery of important nutrients to the brain. The BBB presents at all levels of the vascular trees and acts as a vast and dynamic interface maintaining the cerebrovascular integrity to allow neurons to function properly [1]. As the core anatomical element of the BBB, brain microvascular endothelial cells (BMECs) have continuous intercellular tight junctions (TJs) and adhesions junctions (AJs) [2] (Fig. 1A). These TJs firmly seal the gaps between BMECs, thus resulting in a high transendothelial electrical resistance (TEER) of approximately 1.8 kΩ/cm² [3], which limits paracellular permeability and regulates the diffusion of lipids and proteins. Occludin and Zonula occludens-1 (ZO-1) are typical TJ proteins, playing a critical role in maintenance of BBB functions, and their high-level expressions are correlated with high TEER and low permeability [4].

The BBB provides a tight control on the passage of nutrients from the vasculature into the extracellular fluid of the CNS, as well as shielding the brain from neurotoxic agents [5]. On the other hand, such substantial barrier presents one of the biggest challenges for drug delivery to the CNS due to low BBB penetration [6]. CNS diseases such as Alzheimer disease (AD), Parkinson disease (PD), glioblastoma, and rabies pose a huge threat to human health [7–9]. Because of TJs seal the BBB, macromolecular drugs in the plasma, such as neutralizing antibody against rabies virus and some glioblastoma, cannot target the virus or the tumor cells in the brain behind the BBB, unless the antibody is physically transported into the brain [10, 11]. Although many new drugs were developed in preclinical studies for treatments of Alzheimer disease, most of them clinically failed because of the low brain penetration, and only a few drugs with high brain penetration showed therapeutic efficacy, which clearly indicates that improving the drug delivery is the key for therapy of neurodegenerative disease [12]. However, drug delivery through the BBB requires reversible disruption of TJs to enable temporary paracellular transport routes between BMECs [13]. Therefore, it is crucial to recover the barrier function following entry of therapeutic agents into the brain to avoid the potential influx of unwanted molecules and immune cells leading to the neurotoxicity. BBB disruption has previously been observed in the symptoms-stage of AD [14], implying that the uncontrolled opening of the BBB accelerates the disease course.

Safe blood-brain barrier opening (BBBO) is critically important in drug delivery, which requires controllably reversible unzipping of the TJs. Current BBBO approaches can be generally categorized into chemical and mechanical ones. Among the mechanical ones, ultrasound can generate mechanical pressure wave with frequencies normally above a few kHz, up to a few MHz. This modality has been broadly used in anatomical imaging and hemodynamic measurement [15, 16]. Focused ultrasound generates and utilises microbubbles to disrupt the TJs via cavitation, leading to transient BBBO [4]. For example, ultrasound working at 220 kHz had been demonstrated to aid the BBBO and temporarily open BBB in patients with Parkinson disease and dementia, resulting in mild cognitive improvement [17]. Bevacizumab delivery assisted by focused ultrasound working at similar frequencies was also reported to significantly retard glioma progression with an increased median survival of 135% [18]. Despite the enhanced BBB permeability was reported, the microbubble cavitation induced BBBO is well-known to be unpredictable and even cause apparent damages to brain tissue [19]. One potential improvement when using the ultrasound to induce BBBO includes increasing the ultrasound frequency up to MHz level, so that the acoustic cavitation is minimal.

Surface acoustic waves (SAWs), with frequencies ranging from a few MHz to a few GHz, have been demonstrated in producing nanoscale surface vibrations with their wavelengths comparable to sizes of biological cells [20]. SAWs propagate along the surface of solid materials with most of their acoustic energy trapped near the surface. This characteristic provides great advantages of integrating micro-systems such as microfluidics on the surface of a SAW device, allowing an effective manipulation of bioparticles in an acoustofluidic system [21]. For example, SAW stimulation accelerated neural differentiation process of human embryonic stem cells with a great potential in shortening the time for cell therapy manufacturing [22]. Acoustic excitation working at MHz levels has also been demonstrated to effectively internalize molecules into cells while maintaining the integrity of both the therapeutic agents and the host cells [23]. The mechanism of cell transfection might be associated with temporal reorganization of the lipid structures in the cell membrane that allowed therapeutic molecules to be delivered [24], and acoustic streaming is supposed to facilitate this process [23]. Another SAW based device working at MHz ranges has been successfully demonstrated to increase the motility of human sperm by 15% [25]. SAW has also been applied to assemble patient-derived cell clusters to model tumour organoids, which can be implemented for personalised cancer adjuvant therapy [26].

To date, transwell systems are one of the simplest and also most commonly seen in vitro BBB models. In this system, a single layer of BMECs is seeded on top of the upper semipermeable membrane, separated from the abluminal (apical) (Fig. 1B). For technical reasons to combine SAW device with in BBB, current rarely researches applying SAW for blood brain barrier opening. In this study, we activated the BBB model on the transwell system by applying a SAW with a frequency about 20 MHz. The proposed acoustofluidic transwell (AFT) acquired the advantage of our open-source acoustofluidic systems based on printed circuit board architecture [27–29], which has merits of rapid prototyping on-demand and versatile in combination other micro-systems. The AFT was operated under various SAW agitation dosages. The preliminary tests clearly demonstrated that the AFT is able to control the opening of the BBB with reversible disruption of TJs. The applicable SAW dosages were demonstrated to create temporary paracellular transport of substances with different molecular weights. The expression of occludin and ZO-1 showed no significant changes, but intercellular space was enlarged after SAW administration. After the treatment of SAWs, the hBMECs were recovered with a restored TEER. Finally, we validated the BBBO using zebrafishes to demonstrate the permeation of sodium fluorescein into the brain tissue. Our results confirmed that the AFT is particularly useful in regulating the BBBO for drugs delivery across the blood-brain barrier.

Working mechanism

The AFT provides fundamental functionalities of the BBB and facilitates delivery tests through the endothelial cells under the assistance of the SAWs, which were generated using a printed circuit board based acoustofluidic bioreactor (PCB-AFB) [22] and a transwell insert as shown in Fig. 1A. Human brain microvascular endothelial cells (hBMECs) were seeded as a single cell layer on the upper side of the porous membrane on the transwell insert as shown in the protocol described in the following session (Fig. 1B). During the treatment, the transwell insert was plugged into the socket on the PCB-AFB. The internal configuration of the AFT is displayed in Fig. 1A, which is detailed in the device fabrication session.

The whole process is illustrated in Fig. 1B. After seeding and culturing the hBMECs (Step 1 and 2), the transwell insert is plugged and submerged in the medium in the socket (Step 3), where the SAWs produced from both sides interact with the hBMECs through the porous membrane. The TJs between endothelial cells are affected, resulting in an enlarged gap between cells. Such ‘opening’ effect can be reflected by measuring the transendothelial electrical resistance (TEER) (Step 4). The TEER is a widely accepted quantitative technique to assess the integrity of TJ dynamics in cell culture models of endothelial monolayers. The enlarged gaps allow pharmaceutical agents to penetrate through from the ‘blood’ side (luminal compartment) to the ‘brain’ side (abluminal compartment) (Step 5). The AFT works as a tool to control the BBBO without the need of any opening agents. The validation of the AFT is performed using a zebrafish model, where the zebrafish is administrated with an appropriate SAW dose to test the penetration of the BBB (Step 6).

Fabrication Of The Acoustofluidic Transwell (Aft)

The AFT is a highly accessible acoustofluidic device fabricated by a number of off-the-shelf components. Figure 1A shows all the components assembled the AFT and the inner architecture of the device. A rapid prototyping of the device is available on demand without the need of cleanroom manufacturing.

A heatsink (① in Fig. 1A, Farnell UK, order code: 1850032) was used as the base support for the AFT and responsible for heat dissipation. The heat exchange between the heatsink and the environment was accelerated by using a DC axial fan (⑭, Farnell UK, order code: 3794029) mounted under the heatsink (①). Two peltier coolers (⑪, Farnell UK, order code: 1639724) were mounted on the heatsink with their cold sides coupled to a customised aluminum plate (②) with a thickness of 1 mm. Heatsink grease (Farnell UK, order code: 1663190) was applied on both sides of the peltier coolers to improve the thermal coupling. Due to poor thermal conductivity of LiNbO₃, a thermal conductive sheet with the thickness of 1 mm made by silicone (Farnell UK, order code: 1893451) was mounted on the top surface of the aluminum plate to couple any heat from a LiNbO₃ wafer (③, 128° Y-cut X-propagation, 3 inch). The interdigital transducer (IDT) function was generated by pressing and finger electrodes patterned on a thin film printed circuit board (PCB) onto a LiNbO₃ wafer (④). The PCB design file was submitted to a PCB manufacturer (circuitfly.com) for fabrication. The PCB substrate was a flexible polyester film with a thickness of 70 µm, on which 40 pairs of finger electrodes with a period of 200 µm and an aperture size of 2 cm were patterned by depositing two metal layers, nickel/gold (2 µm/30 nm thick). To firmly couple the PCB to the LiNbO₃ wafer, silicone pads (⑤) and aluminum pressors (⑥) were stacked onto the PCB using additional mechanical structure (not shown in Fig. 1A) to enforce the contact between the finger electrodes and the wafer.

Amplified radio frequency signals were fed into both the IDT and converted into SAWs propagating on the surface of the LiNbO₃ wafer. A compartment of PLA plastic was 3D printed for accommodating the transwell insert and storing medium for the ‘brain’ side (⑦). The volume of the ‘brain’ side was 12 mL for fully submerging the transwell insert, which contacted with the LiNbO₃ wafer for coupling acoustic waves to the hBMECs (⑧) on the membrane. The TJs on the hBMEC were disrupted by the acoustic energy, whose opening can be quantified by measuring the electrical resistance across the hBMEC monolayer separating the ‘blood’ side (⑨) to the ‘brain’ side (⑦). The TEER meter (⑬) read the resistance between its two electrodes (⑩), which indicate the integrity and permeability of the hBMEC monolayer.

Device Characterization

The width and spacing of the finger electrodes on the PCB are key parameters determining the working frequency of the AFT. After manufacturing, the integrity and accuracy of the finger electrodes were checked under a measuring microscope (Soptop, CX40M). The AFT consisting of two IDTs was treated as a two-port network whose S-parameters were measured using a vector network analyzer (VNA, E5061B, Keysight, US). As shown in Fig. 1C, the reflection coefficient and the insertion loss are monitored to indicate the assembly quality and alignment of the two IDTs, respectively. Measuring the reflection coefficient is essential to inform the process of impedance matching for the IDTs [30]. The dip frequency read from the reflection coefficient S₁₁ / S₂₂ was registered as the working frequency of the AFT. A temperature control was used to maintain the temperature in the transwell insert under different SAW doses. The choice of these SAW doses was demonstrated by measuring the microparticles streaming velocity using an optical the microscope.

Device Modelling

To evaluate the acoustofluidic field in the AFT, a 2D model of the device was built in COMSOL Multiphysics 5.5. (COMSOL Inc., USA). Ten pairs of IDTs were modelled on the LiNbO₃ wafer. The transwell and support beams were modelled as linear elastic materials made of PLA plastic, and the bottom of the transwell was selected as porous polycarbonate. The acoustic pressures generated by leaky SAWs were obained under the Thermoviscous Acoustics module in the frequency domain. Governed by incompressible Navier-Stokes equations, the laminar flow module was used to calculate the drag and acoustophoretic forces as well as liquid displacement effects due to the previously calculated acoustic pressure. Forces obtained in the liquid were coupled with a time domain study and particle tracing module, to observe cell barrier deformation.

Cell Culture And Transwell Bbb Model

hBMECs were maintained in RPMI 1640 medium supplemented with 10% (vol/vol) fetal bovine serum (FBS, Gibco, Grand Island, NY), penicillin/streptomycin, endothelial cell growth supplement (L-glutamine, sodium pyruvate, amino acid, vitamin) as previously described with modification [31]. The in vitro BBB model was adapted from established one according to reported scheme [32, 33]. Briefly, a 6.5 mm transwell (Costar, Corning, USA) was coated with collagen Ⅳ, then the hBMECs were seeded in the transwell inserts with the population of 8⋅10⁴ cells. After the cells reach 100% confluent (24 h and 48 h), the transwell inserts were either left untreated or exposed to SAWs with various doses.

Teer And Transendothelial Permeability

The TEER was measured with an EndOhm electrodes (World Precision Instruments, WPI, Sarasota) combined with Millicell ERS-2 resistance meter (Millipore, Billerica, MA) according to the manufacturer's instructions. For transendothelial permeability assay, 100 µg/ml NaF, 1 mg/ml FITC-dextran-10 kD (Sigma-Aldrich, St. Louis, MO), or 1 mg/ml FITC-dextran-10 kD (Sigma-Aldrich, St. Louis, MO) were added to the up chamber of transwell insert after SAW stimulations. After 5 min incubation, the fluorescence density of upper chamber and lower chamber were measured at excitation at 488 nm and emission at 530 nm.

Cell Viability Detection

The confluent hBMECs were exposed to SAWs with various powers, then the CCK8 reagent (Biosharp Life Science, China) was added into each well. After 1 h incubation at a 37℃ incubator, the absorbance was detected with a microplate reader at a wavelength of 450 nm.

Western Blotting And Immunofluorescence

Western blotting and Immunofluorescence

The hBMECs being exposed to the SAWs were harvested with RIPA buffer containing protease inhibitor. A BCA kit (Beyotime, China) was used to measure and adjust the protein concentrations. The cell lysis was mixed with loading buffer and incubated at boiling water for 10 min. Samples were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. The proteins in gel were transferred to polyvinylidene difluoride membranes (Millipore). After blocking with 5% skim milk, the membrane was hybridized with anti-occludin, ZO-1, or β-actin (Proteintech, China) antibody. The signal was amplified by conjugating to HRP-labeled second antibody, and results was observed with ECL chromogenic solution (Beyotime, China).

For immunofluorescence, the hBMECs were fixed with 4% paraformaldehyde. Cells were then blocked with 2% BSA, and anti-occludin or anti-ZO-1 polyclonal antibody (Proteintech, China) was applied to mark the targeted proteins, followed by conjugation using Alexa Fluor 488 labeled second antibody. The images were captured on a laser confocal microscope (Leica SP8, Germany).

Ten-day-old zebrafish (Danio rerio, 10 days post fertilization, transparent body) were kept in 1 mg/ml NaF water of AFT system, and exposed to SAWs under an input power of 37.8 dBm (about 6 W), while the control was sustained in 1 mg/ml NaF water. After exposed to the SAW agitation for 5 min, the zebrafish was washed in fresh water and the fluorescence in the brain was imaged using a fluorescence microscope (Olympus IXplore, Japan). After experiment, all zebrafish were euthanized by a full dose of tricaine methanesulfonate (Sigma) and safely disposed.

Statistical analysis

Data of the study are showed as mean ± standard error of mean (SEM) unless otherwise stated. The significance was tested with two tailed t test, one-way or two-way analysis of variance (ANOVA) followed by Tukey’s post hoc tests with GraphPad Prism software (v7.0; GraphPad, La Jolla, CA). The results are the representative of at least two independent experiment which was detailed in the figure legends.

Numerical simulation and characterization of the AFT

The acoustofluidic modelling of the AFT displays the SAWs to propagate through the porous membrane on the transwell insert and arrive the hBMECs. As the simulation result shown in Fig. 2, the SAW leaks into the medium underneath the porous membrane, which creates a first-order acoustic pressure field inside the abluminal compartment. The acoustic waves encounter with the porous membrane and convert into the membrane vibrations, exerting mechanical stimulation to the attached hBMECs. The TJ proteins between the hBMECs are considerably disrupted by the mechanical vibrations. The acoustic energy is then passed through the cells and finally absorbed in the luminal compartment inside the transwell insert, which produces intensive streaming to enhance the collisions between the pharmaceutical agents and the hBMEC.

The finger electrodes on the PCB are the key element involving coupling radio frequency (RF) signals to the piezoelectric substrate for generating mechanical vibration. The measured IDT width and spacing were 31.0±1.1 µm and 67.6±0.9 µm, respectively, as shown in Fig. 3A, resulting in the SAW wavelength of 197.1±2.4 µm. The working frequency was firstly determined by reading the S₁₁ and S₂₂ spectrum as shown in Fig. 3B, where the reflection of the IDTs was reduced from − 2.5 dB to -53.5 dB, and − 2.4 dB to -49.4 dB, respectively, indicating the power transmission was greatly improved by introducing the matching network. The working frequency was set as that produced the minimum reflection, i.e., 19.68 MHz.

The thermal effect in the luminal compartment should be well managed to minimise the impact on the attached hBMECs. The temperature rise inside the compartment was within 0.5°C under the SAW doses applied during the SAW treatment. As shown in Fig. 3C, measured streaming velocities polystyrene microparticles (size 10 µm) was fully controllable by adjusting the AFT input power.

Enhancement Of Bbb Permeability In The Aft

The insert was coated with type Ⅳ collagen protein, which mimicked the basement membrane of the BBB. Then hBMECs were seeded onto transwell insert to form the hBMEC monolayer (Fig. 4A). Usually, the in vitro BBB was formed after 24 h of cell culture, which is characterized by high TEER, normally noted above 30 ohm·cm² in a 24-well transwell plate. After 24 h of cell confluence, the TEER was kept raising because of the cell proliferations, and at about 48 h, a tight BBB was established. The in vitro BBB was treated on AFT with various SAW powers. BBB disruption was immediately noted after SAW stimulations. The TEER of the hBMECs was found to decrease with SAW agitation with power of 20 dBm, 25 dBm, or 30 dBm, which began at the end of SAW stimulation (for 5 min) and then continued up to 4 h (Fig. 4B). Moreover, after overnight incubation, the TEER values of all groups recovered with notable increase at 24 h. The TEER showed a slow reduction after 72 h, which might be due to highly crowded cells consuming the medium nutrition. To explore the relationship between the SAW duration and BBB disruption, the dose of 30 dBm was selected for the following study. The SAW duration was set for 1 min, 3 min, or 5 min, respectively. Similar results were observed that the TEER decreased after SAW agitation for 5 min and TEER value sustained up to 4 h (Fig. 4C). After 24 h, the TEER of hBMECs was resumed completely, reflecting the recovery of the barrier function. To further investigate the susceptibility of BBB to SAW agitation, different status BBB were exposed to SAWs. We observed that a low TEER of BBB was sensitive to SAWs and BBB opening occurred at power of 30 dBm (Fig. 4D). However, but for high TEER of BBB, a higher power of SAW such as 37.8 dBm was required for opening of BBB (Fig. 4D). These results clearly proved that SAW treatment changed the TEER of BBB, but for a tight BBB, a high power SAW was essential for altering the BBB permeability and the BBB was recovered far more rapid.

Saw Opened Bbb Without Affecting Cell Viability Of Hbmecs

To determine whether SAW exposure would alter the cell viability of hBMECs, a CCK8 kit was applied. The hBMECs were seeded in transwell inserts for 48 h to form the firm BBB, then the inserts were treated with SAW agitations at 35 dBm or 37.8 dBm, for 5 min at a frequency of 19.68 MHz. Results showed that the TEER of hBMECs was dramatically decreased (Fig. 5A), while the cell viability of hBMECs was about 93.1% and 85.1% after 35 dBm or 37.8 dBm SAW treatments (Fig. 5B). Observed using optical microscope, cell morphology of hBMECs turned to be rounder under 35 dBm or 37.8 dBm SAW exposure, but did not show dramatic differences, if compared with that of control group (Fig. 5C). These results suggested SAW stimulation barely affected hBEMCs cell viability, but slightly changed the cell morphology.

Saw Stimulation Increased The Permeability Of Chemical Drugs Cross The Bbb

Fluorescein sodium (NaF) and fluorescein-labeled dextran are generally used as indicators to test the BBB permeability [32, 34]. In this study, NaF (376 Da), FITC-dextran-10 kD, and FITC-dextran-70 kD were selected, which represented the small molecule and macromolecular chemical drugs, respectively. To evaluate the transendothelial permeability of BBB to drugs with different molecular weights, the transwell inserts were exposed to SAW (with a frequency of 19.68 MHz) at a power of 37.8 dBm for 5 min, and then NaF or FITC-dextran was added to the upper chamber of inserts as illustrated in Fig. 4A. It was observed that all of NaF, FITC-dextran-10 kD, and FITC-dextran-70 kD in the lower chamber showed an increase of permeation after SAW treatment in AFT device (Fig. 6A). The ratio of fluorescence intensity in lower chamber to upper chamber exhibited about 1.5-, 2.6-, and 54.9-folds increase respectively in NaF, FITC-dextran-10 kD, and FITC-dextran-70 kD group compared with the control group (Fig. 6A). BBB disruption was usually associated with the down regulation of TJ proteins [35]. The expression of occludin and ZO-1 was determined by western blotting. There were no significant changes observed on ZO-1 and occludin expression after SAW treatment (Fig. 6B). These data clearly implied that SAW agitation enhanced BBB permeability, and the BBB disruption was independent of the expression regulation of TJ proteins.

Saw Damaged Bbb Integrity By Enlarging The Intercellular Space Of Hbmecs

To further explore the mechanisms that related to SAW induced BBB breakdown, the immunostaining was performed to determine the expression and distribution of TJ proteins on hBMECs. The hBMECs were seeded on slides for 48 h, then the slides were exposed to SAW at 19.68 MHz, 37.8 dBm for 5 min and subjected to immunostaining promptly. Occludin and ZO-1 were continuously expressed in the cell membrane encircling the cells and delineating the cellular borders of hBMECs, while small fraction of the occludin was also expressed in cytoplasm (Fig. 7Aa, 7Ba, Fig. S1). As reported, once the BBB was formed in vitro, TJ sealed the intercellular space (red dotted line marking area) maintaining a lower paracellular permeability (Fig. 7Ab, 7Bb, and Fig. S1). Once exposed to SAW on the AFT device, the interval between adjacent cells (e.g., white dotted line marking area) were unzipped and showed enlarged intercellular space (Fig. 7Ae, 7Be, and Fig. S1), corresponding to the permeability increase as shown in Fig. 6A. These results demonstrated that the SAW did not affect the expression of TJ proteins but disrupted the TJs connection among hBMECs, resulting in an increased paracellular permeability.

Saw Exposure Promoted Naf Permeation Into Zebrafish Brain

For further investigation whether SAW facilitate in vivo drugs delivery, a zebrafish model was applied. The 10-day-old (i.e., 10 days post fertilization) zebrafish were applied because they are clearly visible for the observation of NaF penetration into the brain of fish with transparent body shapes under the fluorescence microscope. The brain areas of zebrafish are generally consisted of four parts. From the anterior to posterior, the zebrafish brain could be dissected to telencephalon, optic tectum, cerebellum and brainstem (Fig. S2) as previously reported [36]. The zebrafish were kept in 1 mg/ml NaF solution with or without SAW stimulation (19.68 MHz, 37.8 dBm) for 5 min, then the zebrafish were observed under the fluorescence microscope immediately. At a low magnification, the full zebrafish were seen, and a strong signal of fluorescence was located in the digestive tract (Fig. 8A, 8B). After zoomed in, the fluorescence signal was visible in zebrafish brain (Fig. 8C, 8D, 8E, 8F). Particularly, with SAW stimulation, the NaF were penetrated into the zebrafish’s brain (white arrow) and were punctate distributed (Fig. 8F). Unexpectedly, we also discovered an increase permeation of NaF to the eyes of zebrafish after SAW exposure (Fig. 8B). Considering that the eyes contain blood–retinal barrier, which is similar with blood-brain barrier, we assumed that enhancement of drug penetration using SAWs was not only limited to the BBB since the whole body of zebrafish exposed to the SAWs. These results clearly showed that SAW exposure led to an increase of barrier permeability in zebrafish, consequently, the NaF penetrated into the zebrafish brain.

The characteristics of BBB pose a tremendous challenge for the delivery of compounds to the brain parenchyma, leading to a low uptake of biotherapeutics and unsatisfactory treatment outcomes. Among multiple strategies, actively opening the BBB using the ultrasound was demonstrated to improve the efficiency of drug delivery, however, cavitation induced brain damage during treatment was usually unavoidable [37]. In this study, we developed the AFT device that was integrated with the transwell model. We found that the preliminary BBB (24 h) was susceptible to SAW exposure, in which a lower SAW power and less time of SAW stimulation was sufficient to enhance BBB permeability. Tight BBB (48 h) was stable under low power of SAWs, however, with the increase of SAW power in the AFT device, the tight BBB showed an increased permeability. The reason for different sensitivities to SAW exposure might be attributed to the TJ proteins between hBMECs on early BBB were relatively loose and became secure after extra time of hBMECs cultivation. By selecting the appropriate power of SAWs, we confirmed it is a promising method for CNS drugs delivery by reversibly opening the BBB.

We observed the integrity of BBB was disrupted with various SAW power and durations in the AFT device. After a short period of culture, the integrity of BBB was recovered by the cell growth, which showed a reversible and controllable process. In fact, the TJ proteins of BBB were dynamic [38], which might recover to normal after moderate destruction. The BBB in organism is relatively stable and insusceptible. Some cytokines and virus such as interferon and West Nile virus even reinforced the barrier function of BBB [39], while the inflammatory factors and peroxynitrite could damage BBB [34, 40]. The tight BBB did not show the permeability changes until exposure to a higher SAW power on AFT device, indicating that the SAW power should be adjusted according to the TEER of BBB. Indeed, the TEER of firm BBB was found to reduce after 35 or 37.8 dBm SAW exposure, but the cell viability still maintained at 85.1% even a 37.8 dBm SAW exposure. However, SAW exposure did show morphology changes of the roundness of hBMECs in the AFT device, leading to an increase intercellular space among cells, which might be responsible for the change of BBB permeability.

BBB disruption was usually related to the degradation of TJ proteins. For example, Interleukin 6 disrupted the integrity of BBB via activating ubiquitin–protein ligase E3 and promoting proteasomal mediated ZO-1 degradation [34, 41]. Another study suggested that the degradation of TJ proteins was possibly correlated with autophagy in an experimental autoimmune encephalomyelitis model [42]. Unexpectedly, we found the expression level of occludin and ZO-1 was almost no visible alterations after SAW exposure in the AFT device, indicating that SAW induced BBB breakdown was not via degradation the TJ proteins. In fact, it is only required a short time for the SAW stimulation on hBMECs (e.g., up to 5 min), it was probably not through the degradation of TJ proteins by proteasome or autophagy route. The mechanism associated BBB disruption after SAW stimulation might be related to the change of cell arrangement. Indeed, we observed the enlargement of intercellular space of the adjacent cells after SAW exposure compared to those of the control group, and we did not see apparent changes of expression of TJ proteins. It is more like unzipping the adjacent cell membranes, which reflected an instantaneous and effective pulling force on the cells. After another 24 h culture, the intercellular space between adjacent cells was disappeared (data not shown), suggested a recovery of BBB. SAW induced BBB disruption might because that the SAW mode we were using was Rayleigh wave mode, which has both a longitudinal and a vertically component [43]. The complex forces of SAW agitations were able to pull and open the adjacent cells. Other ways that disrupted BBB were possibly electromagnetic fields, thermal effects, and acoustic streaming [44]. Local excessive heating generated by SAWs might change the state of the cells [45], and cell shapes were believed to affect under periodic acoustic streaming by SAW excitation [46]. More studies are needed to clarify the specific mechanism of SAW induced BBB breakdown.

However, there are also inadequacy results in the current study. The limit of acoustic instrument and the thermogenesis of piezoelectric LiNbO₃ restricted the high power of SAW device. A semiconductor temperature reducer was used in our study when the BBB exposed to a high power of SAW. The TEER detection and transendothelial cell permeability measurement were only performed at the given time of the experiment and not a real time monitoring process. In this way, the slight inflection of the BBB integrity could not be easily observed. The in vivo trial showed a relatively weak fluorescence signals in zebrafish brain, which might because the AFT device was not fully adapted to the alive and kicking fish. The energy of SAW wave in AFT device has been attenuated after crossing the medium, skin and brain parenchyma to microvessel wall of zebrafish. We observed the penetration of NaF to the eyes of zebrafish, suggesting that SAW of AFT device could also disrupt blood–retinal barrier. The eyes showed a much higher fluorescence intensity than that in the brain. This might because the eyes were not fully covered by the skeleton, and the skin around eyes was so thin, that the SAW was easily accessible to the barrier sites.

In general, this research applied the SAW device to stimulate the BBB which was not reported before. The SAW platform can be applied as a useful tool that reversibly opens the BBB without damages of the long-term barrier function of BBB, which should be developed as an alternative pathway for CNS drug delivery.

AFT: acoustofluidic transwell; BBB: blood-brain barrier; BBBO: blood-brain barrier opening; CNS: central nervous system; hBMECs: human brain microvascular endothelial cells; SAW: surface acoustic wave; TJs: tight junctions; TEER: transendothelial electrical resistance.

Acknowledgements

We thank Dr. Zhen Xu ( Institute of Hydrobiology, Chinese Academy of Sciences) for kindly providing the zebrafish.

Funding

The authors are grateful for the financial support provided by the National Program on Key Research Project of China (SQ2022YFD1800030), the National Natural Sciences Foundation of China (Grant No. 31872455) (M.C), as well as the UK Engineering and Physical Sciences Research Council (EPSRC) under grant EP/P018998/1, and International Exchange Grant (IEC/NSFC/201078) (Y.F) through Royal Society UK and the NSFC.

Authors’ contribution

K.W and M.C wrote the manuscript with the support of X.Y. X.Y and M.C designed the experiments. C.S made the surface acoustic wave device. K.W performed the experiment with the help of C.S, and H.Z. P.D and Z.W aided in the simulation analysis. L.T, W.P, J.W, and M.C kindly provided the proposal of surface acoustic wave device design. Y.Z and X.L aided in the establishment of blood brain barrier model. X.Y, M.C and Y.F revised the manuscript. All the authors read and approved the final manuscript.

Ethics statement

The zebrafish study was performed according to Institutional Animal Care and Use Ethics Committee of Huazhong Agricultural University (Wuhan, Hubei, China). All procedures involving animal experiments were following the protocol of the Guide for the Care and Use of Laboratory Animals of Huazhong Agricultural University (permission number: Fish-2022-05-24).

Consent for publication

Not applicable.

Availability of data and materials

The datasets used during the current study are available from the corresponding author on reasonable request.

Competing of interests

The authors declare no conflicts of interest.

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Open Source Board Based Acoustofluidic Transwells for Reversible Disruption of the Blood-Brain Barrier for Therapeutic Delivery

Status:

Journal Publication

Version 1

Abstract

Background

Methods

Results

Conclusions

Figures

Introduction

Materials And Methods

Working mechanism

Fabrication Of The Acoustofluidic Transwell (Aft)

Device Characterization

Device Modelling

Cell Culture And Transwell Bbb Model

Teer And Transendothelial Permeability

Cell Viability Detection

Western Blotting And Immunofluorescence

Statistical analysis

Results

Numerical simulation and characterization of the AFT

Enhancement Of Bbb Permeability In The Aft

Saw Opened Bbb Without Affecting Cell Viability Of Hbmecs

Saw Stimulation Increased The Permeability Of Chemical Drugs Cross The Bbb

Saw Damaged Bbb Integrity By Enlarging The Intercellular Space Of Hbmecs

Saw Exposure Promoted Naf Permeation Into Zebrafish Brain

Discussion

Conclusions

Abbreviations

Declarations

References

Supplementary Files

Status:

Journal Publication

Version 1