Allometric normalization of handgrip strength in older adults: which body size parameter is the most appropriate?

doi:10.21203/rs.3.rs-2338198/v1

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Allometric normalization of handgrip strength in older adults: which body size parameter is the most appropriate?

https://doi.org/10.21203/rs.3.rs-2338198/v1

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published 08 Feb, 2023

Read the published version in BMC Sports Science, Medicine and Rehabilitation →

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Background

Although absolute handgrip strength has been associated with health-related outcomes in older adults, little evidence has been provided regarding its adjustment by a variety of body size dimensions. Therefore, the main purpose of the study was to establish the most appropriate normalization of handgrip strength by different body size parameters in a large sample of noninstitutionalized older adults.

Methods

In this cross-sectional study, we enrolled 643 men and women aged > 60, who were part of the rehabilitation center facility program. Handgrip strength was objectively measured using a Jamar Plus* + Digital Hand Dynamometer. Body size parameters included body weight and height, body mass index, waist circumference, waist-to-height ratio, fat mass and fat-free mass. The most appropriate parameter associated with handgrip strength was identified using allometry.

Results

Findings showed that the most appropriate body size parameter for handgrip strength normalization was height (allometric exponent: 0.85), compared to fat-free mass (0.26) and body mass (0.12). Other body size variables were not significantly associated with handgrip strength and were omitted from further analyses. The correlations between normalized handgrip strength were significant when handgrip strength was normalized by body mass and fat-free mass, while no significant correlations were found, when handgrip strength was normalized by body height.

Conclusions

Based on the study results, body height seems to be the best body size parameter for handgrip strength normalization in older adults, omitting the influence of body size on strength performance. If handgrip strength is measured, body height may help normalize strength for large-scale research.

Allometric modelling

Elderly population

Dynamometer

Body size

Performance

Aging process is associated with a reduction of muscle strength [1]. Muscle strength is considered a key element for physical performance and independence in older adults [2]. Evidence shows that lower level of muscle strength leads to higher incidence of cardiovascular [3], metabolic [4], and cerebrovascular diseases [5], falls [6], early hospitalization [7] and premature mortality [8].

There are many alternatives for assessing muscle strength in older adults which include tools and methods that measure voluntary movements related to strength [9]. Handgrip strength is a simple and easily utilized surrogate of the maximum voluntary contraction force of the hand for the assessment of muscle function [9,10]. Low handgrip strength has been associated with several poor health-related outcomes such as functional deficits, cognitive declines, chronic morbidity and premature mortality [11]. Thus, its applicability has become important in clinical and epidemiological settings.

The measure of handgrip strength is often presented as an absolute (non-normalized) value. This would imply that larger individuals would have an advantage over smaller ones, if the results are not correctly normalized [12]. To overcome this problem, previous evidence in older adults has proposed that the ratio of the absolute handgrip strength should be normalized by different body size parameters such as body weight [11,13–17], body height [13,14,17], body mass index [13,15], fat-free mass [14] and waist circumference [17]. This approach is usually called ‘allometric scaling’ and represents the method used for taking into account the body size effect [18,19]. As one would expect, the most common variable used in allometric adjustments is body mass [16]. However, little is known about different body size parameters being compared to assess the most appropriate variable to be used in the normalization model of handgrip strength in older adults [14]. The most recent study by Nevill et al. [17] showed that neither body mass nor body mass index were appropriate to normalize handgrip strength, yet height was shown to be the best body dimension associated with handgrip strength. Also, only a small number of previous studies have used sex [14,20] and age [14,21] in the allometric models, although their influence has been well described in the literature.

Therefore, the main purpose of the study was to establish the most appropriate body size dimension by comparing the effects of body weight, body height, body mass index, waist circumference, waist-to-height ratio, fat mass and fat-free mass to normalize handgrip strength in a large sample of noninstitutionalized older adults. Based on previous evidence [14,17], we hypothesized that body height would be more appropriate than other variables for being used on the isolation of body size influence.

Study participants

Our sample size was based on measurements taken from 2020 to 2022 for 1,000 participants. The inclusion criteria for participation in the study included a self-reported information on: (i) being without chronic diseases, which included chronic heart disease, rheumatic arthritis, chronic kidney disease, stroke, cancer and chronic obstructive pulmonary disease, (ii) the absence of a serious physical or mental illness, and (iii) having all the study variables tested. Of 1,000 participants, 840 met the inclusion criteria. After re-analysis, 643 men and women had all the study variable measurements and were included in the final analysis. Before data collection started, all participants were informed about the aim, hypotheses and methodology of the study. The participants were ensured confidentiality and informed that their participation was voluntary, and that they had the right to withdraw at any time. All participants have read and signed the informed consent forms. We followed the methods of the principles of the Declaration of Helsinki [22], and the Ethical Committee of The Home of War Veterans approved the study (Ethical code number: 2022/4).

Handgrip strength measurement

To assess handgrip strength, we used a Jamar Plus* + Digital Hand Dynamometer (Sammons Preston Inc., Bolingbrook, Illinois, USA). The device was calibrated by the manufacturer, with a precision of 0.1 kg. The protocol for measuring handgrip strength was followed by the American Society of Hand Therapists [23]. In brief, the participant was placed in a seated position with shoulder rotated and adducted in a neutral position, forearm in neutral position, elbow flexed at 90° and wrist between 0 and 30° of dorsiflexion. Each participant conducted the measurement three times with the non-dominant hand [23]. Out of three measurements, the best one was recorded and used in further analyses.

Anthropometric measurement

Body height and weight were objectively measured using Seca portable stadiometer and digital scale with a precision of 0.1 cm and 0.1 kg. Body height was measured in bare or stocking feet standing upright against a stadiometer and body weight with wearing light clothes with no shoes. Body mass index was calculated (weight [kg]/height [m]²). Waist circumference was measured using anthropometric tape placed horizontally midway between the lower rib margin and the iliac crest at the end of normal expiration, while the participant was standing still [24]. Waist-to-height ratio was calculated by dividing waist circumference with body height (in cm). To assess fat mass and fat-free mass, we used bioelectrical impedance analysis (Omron BF500 Body Composition Monitor, Omron Medizintechnik, Vernon Hills, IL, USA). The device uses eight electrodes and pre-programmed equations to determine fat mass. The participant was required to stand on metal footpads barefoot and grasp a pair of electrodes fixed on a handle with arms extended in front of the chest [25]. Sex and age were self-reported.

Statistical analysis

The statistical analyses were conducted using Statistical Packages for Social Sciences version 23 (SPSS Inc., Chicago, IL, USA). Descriptive statistics were calculated for all variables. Continuous variables were presented as means and standard deviations (SD). Sex differences were examined with Student t-test for independent samples. The magnitude of the differences between the sexes in each variable was calculated using Cohen’s D effect size (ES). According to Hopkins et al. [26], ES was classified as trivial (< 0.2), small (0.2–0.6), moderate (0.6–1.2), large (1.2–2.0), very large (> 2.0) and extremely large (> 4.0). An allometric scaling was done by including the natural logarithm (ln) of each body size dimension as the independent variable against the natural logarithm of handgrip strength as the dependent variable into the log-regression model. Sex (1 for men and 2 for women) and age (a continuous variable) served as covariates in each model. In final analysis, ln body size*sex*age interaction terms were entered and if the interaction terms were not statistically significant, they would be excluded. When the regression equation for each body size and handgrip strength was determined, we tested the data for multicollinearity using the variance inflation factors, normality of residuals using the normal probability plot and histogram of residuals and heteroscedasticity using the standardized residuals versus predicted plot. The variance inflation factors in our model ranged from 1.03 to 2.12 indicating no multicollinearity and the other assumptions were also met. Next, a Pearson correlation analysis was performed between the body size variables and normalized handgrip strength. If the adjustments were significant (p < 0.05), the model would be considered inadequate, because the model was incapable of isolating the body size effect on the handgrip strength. Two-sided p-values were used, and significance was set at α < 0.05.

The Kolmogorov-Smirnov test showed that all study variables were normally distributed. Basic descriptive statistics of the study participants according to sex are presented in Table 1. Men were taller, heavier and had higher body mass index and fat-free mass values, compared to women (p < 0.05). Women exhibited lower waist circumference, but higher fat mass values, compared to men (p < 0.05). The largest effects in absolute handgrip strength were observed, where men generated ≈ 35.0% higher values.

Table 1

Basic descriptive statistics of the study participants presented as mean (SD)
Study variables	Men (N = 260)	Women (N = 383)	ES	P for sex
Age (years)	67.4 (5.5)	66.9 (5.2)	0.09	0.160
Height (cm)	172.9 (5.0)	161.1 (6.0)	2.14	< 0.001
Weight (kg)	84.0 (10.3)	70.0 (12.1)	1.25	< 0.001
Body mass index (kg/m²)	27.7 (3.3)	26.9 (4.2)	0.21	0.027
Waist circumference (cm)	100.1 (9.3)	90.5 (11.6)	0.91	< 0.001
Waist-to-height ratio	0.58 (0.1)	0.56 (0.1)	0.20	0.033
Fat mass (%)	31.2 (7.0)	38.2 (6.6)	1.03	< 0.001
Fat-free mass (%)	70.9 (4.6)	61.8 (6.6)	1.60	< 0.001
Handgrip strength (kg)	46.9 (7.6)	30.5 (5.3)	2.50	< 0.001
P < 0.05

Table 2 shows regression coefficients for the log-transformed independent variables. The separate interaction terms representing the associations between ln handgrip strength with ln body mass index (p = 0.099), ln waist circumference (p = 0.367), ln waist-to-height ratio (p = 0.067) and ln fat mass (p = 0.078) were not statistically significant, so we omitted them from further analyses. The final three regression models were based on body mass, body height and fat-free mass additionally adjusted for sex and age. Allometric adjustments for each model were generated, where β coefficient served as an exponent. The obtained exponents were 0.12 for body mass, 0.85 for body height and 0.26 for fat-free mass. The following equation using the exponents should be applied: “adjusted handgrip strength = absolute handgrip strength (kg)/body size variable^exponent”.

Table 2

Allometric scaling of handgrip strength and body size variables, adjusting for sex and age (N = 643)
Study variables	β (SE)	95% CI	p-value	VIF	R	R²
Model 1
Constant	1.92 (0.12)	1.69–2.16	< 0.001		0.79	0.63
Ln body mass	0.12 (0.05)	0.01–0.22	0.029	1.38
Sex	-0.19 (0.01)	-0.20 - -0.17	< 0.001	1.41
Age	-0.004 (0.001)	-0.006 - -0.003	< 0.001	1.03
Model 2
Constant	1.65 (0.08)	1.50–1.81	< 0.001		0.82	0.67
Ln body height	0.85 (0.10)	0.64–1.05	< 0.001	2.08
Sex	-0.14 (0.01)	-0.16 - -0.12	< 0.001	2.12
Age	-0.004 (0.001)	-0.005 - -0.003	< 0.001	1.04
Model 3
Constant	1.66 (0.17)	1.33–1.99	< 0.001		0.80	0.64
Ln fat free mass	0.26 (0.08)	0.10–0.43	0.002	1.51
Sex	-0.18 (0.009)	-0.20 - -0.16	< 0.001	1.54
Age	-0.004 (0.001)	-0.006 - -0.003	< 0.001	1.03
P < 0.05

The correlations between adjusted handgrip strength and body size variables are presented in Table 3. When the handgrip strength was normalized by body mass, small but significant correlations with height and fat-free mass were observed (p < 0.05). Similar effects were shown for handgrip strength normalized by fat-free mass and body weight and height (p < 0.05). However, the correlations between normalized handgrip strength by body height and the body size variables were not statistically significant, implicating that this model might be adequate for mitigating the body size effect on the handgrip strength.

Table 3

Correlations between adjusted handgrip strength with body size variables, adjusted for sex and age
Study variables	Pearson’s r	p-value	Cohen’s D
Handgrip strength/body mass^0.12
Body mass (kg)	-0.002	0.965	trivial
Body height (cm)	0.32	< 0.001	small
Fat-free mass (%)	0.24	< 0.001	small
Handgrip strength/body height^0.85
Body mass (kg)	-0.04	0.468	trivial
Body height (cm)	-0.004	0.939	trivial
Fat-free mass (%)	0.01	0.847	trivial
Handgrip strength/fat-free mass^0.26
Body mass (kg)	0.22	< 0.001	small
Body height (cm)	0.26	< 0.001	small
Fat-free mass (%)	0.006	0.896	trivial
P < 0.05

The main purpose of the study was to examine and compare the performance of different body size dimensions (body weight, body height, body mass index, waist circumference, waist-to-height ratio and fat-free mass) in the normalization of handgrip strength and adjusted for sex and age in a large sample of older adults. Our main findings are: (i) body height is the most appropriate body size parameters for being used in allometric scaling of handgrip strength, followed by fat-free mass and body weight; (ii) the interaction terms between body mass index, waist circumference, waist-to-height ratio and fat mass with handgrip strength are not significant; and (iii) body mass and fat-free mass show residual associations with allometrically normalized handgrip strength, pointing out that these variable are incapable to completely exclude the body size effect on the handgrip strength.

Evidence recommends that handgrip strength should be normalized for body size dimensions [9,12]. The most common body size dimensions include weight [11,13–17], body height [13,14,17], body mass index [13,15], fat-free mass [14] and waist circumference [17]. Our finding of body mass exponent being 0.12 is smaller, compared to other studies conducted in older adults [12,14,16,27]. For instance, a study by Pua [16] showed larger exponents for body mass and handgrip force (0.63), ankle dorsiflexion force (0.82), ankle dorsiflexion torque (0.91) and Timed “Up & Go” Test (0.07). A study by Maranhao Neto et al. [14] found that body mass exponent for handgrip strength was 0.31, while Foley et al. [27] reported 0.40 in the body mass exponent for handgrip strength allometric normalization. Regarding fat-free mass, our results showed the exponent of 0.26, which is lower than those obtained in previous studies [14,28]. Indeed, Folland et al. [28] presented the findings, where fat-free mass exhibited higher exponents for force (0.76) and torque (1.12) and determined on average 54.0% of the variation in knee extensor torque. When the regression model was adjusted for sex, age and the level of physical activity, Maranhao Neto et al. [14] found 0.46 in the fat-free mass exponent. Our results obtained in the body height exponent (0.85) were like previous findings of 0.92 [28] and 1.1 [29]. However, higher body height exponent of 1.84 was demonstrated for Brazilian older adults [14]. The discrepancy between the exponents for different body size parameters may be due to different sample size, wide age range, adjustment for specific variables and testing measurements of handgrip strength, not being able to generalize the findings and its representativeness. Although we followed the recommendations from the American Society of Hand Therapists for testing handgrip strength [23], previous protocols have used the handgrip strength measured with the elbow in extension [14]. Nevertheless, this is the first study comparing a variety of body size parameters in relation to absolute handgrip strength and adjusting for sex and age in a relatively large sample of older adults.

Greater muscle strength is an important component of preserving from cardiometabolic, locomotor and mental diseases [11]. While the applicability of testing handgrip strength and its normalization has been well-documented in the literature [9,10], studies often fail to adjust for body size parameters with the misleading results. This study has shown that by using body height as the most appropriate body size dimension for allometric scaling, it is possible to provide an unbiased body size adjustment in elderly population.

This study is not without limitations. First, by using a cross-sectional design, we cannot determine the causality of the association between body size dimensions and handgrip strength. Second, to secure a homogenous sample on which allometric scaling was performed, this study was limited to a sample of free-living community-dwelling older adults without locomotor and mental diseases and who did not fall in the past year. Third, although we included fat mass and fat-free mass as body size dimensions, we used bioelectrical impedance analysis and more objective methods, like DEXA or computer tomography might have given different exponents. Fourth, the results of maximal testing are co-dependent with the maximal effort of participants, and because of the nature of the study, this cannot be fully guaranteed.

This study demonstrates that among various body size parameters, body height seems to be the most appropriate allometric variable which completely remove the effect of body size on muscle strength performance in older adults. By using a relatively large sample size of older adults aged 60–80 years, we were able to generate the body exponential value of 0.86 for normalization of handgrip strength. Thus, the following equations should be considered in clinical and epidemiological settings in similar populations: “corrected handgrip strength = absolute handgrip strength (kg)/body height (m^0.85)”. The newly developed allometric equation should be used in older adults when testing handgrip strength.

Ethics approval and consent to participate: The study was approved by the Ethical Committee of The Home of War Veterans (Ethical code number: 2022/4). The informed consent voluntarily was signed by the participants.

Consent for publication: The informed consent voluntarily was signed by the participants.

Competing interests: The authors declare that they have no competing interests.

Availability of supporting data: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Funding: The paper was self-funded.

Contributions: ZK and LŠ conceived the study. ZK, PS and LŠ requested ethical approval, conducted the participant recruitment and performed the physiologic testing and data analysis. LŠ supported the assessment of cases regarding data inclusion, threshold determination as well as data preparation. LŠ wrote the first draft of the article. All authors (MK, PS, ZK, LŠ, AH and PK) revised it critically for important intellectual content, final approval of the version to be published, and accountability for all aspects of the work. All authors read and approved the final manuscript.

Acknowledgements: The authors would like to thank all the participants for their enthusiastic participation in the study.

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No competing interests reported.

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Journal Publication

published 08 Feb, 2023

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You are reading this latest preprint version

Allometric normalization of handgrip strength in older adults: which body size parameter is the most appropriate?

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Journal Publication

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Abstract

Background

Methods

Results

Conclusions

Introduction

Materials And Methods

Study participants

Handgrip strength measurement

Anthropometric measurement

Statistical analysis

Results

Discussion

Conclusion

Declarations

References

Additional Declarations

Status:

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