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Accurate Determination of Genotypic Variance of Cell Wall Characteristics of a Populus trichocarpa Pedigree Using High-Throughput Pyrolysis-Molecular Beam Mass Spectrometry
Anne Elizabeth Harman-Ware
National Renewable Energy Laboratory
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7927-9424
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Background Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer composition. Instrumental and microspatial environmental variability were observed in the spectral features and corrected to reveal underlying genetic variance of biomass composition.
Results Lignin-derived ions (including m/z 124, 154, 168, 194, 210 and others) were highly impacted by microspatial environmental variation which demonstrates phenotypic plasticity of lignin composition in Populus trichocarpa biomass. Broad-sense heritability of lignin composition after correcting for microspatial and instrumental variation was determined to be H2 = 0.56 based on py-MBMS based on ions known to derive from lignin. Heritability of lignin monomeric syringyl/guaiacyl ratio (S/G) was H2 = 0.81. Broad-sense heritability was also high (up to H2 = 0.79) for ions derived from other components of the biomass including phenolics (e.g., salicylates) and C5 sugars (e.g., xylose). Lignin and phenolic ion abundances were primarily driven by maternal effects, and paternal effects were either similar or stronger for the most heritable carbohydrate-derived ions.
Conclusions We have shown that many biopolymer-derived ions from py-MBMS show substantial phenotypic plasticity in response to microenvironmental variation in plantations. Nevertheless, broad-sense heritability for biomass composition can be quite high after correcting for spatial environmental variation. This work outlines the importance in accounting for instrumental and microspatial environmental variation in biomass composition data for applications in heritability measurements and genomic selection for breeding poplar for renewable fuels and materials.
Keywords
biomass composition, poplar, heritability, pyrolysis-molecular beam mass spectrometry, phenotypic plasticity
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- 7x7MBMSheritSupplementaryMaterialfinal.pdf
Supplementary Material
- SuppTable3MZannotations.xlsx
Supplementary Table 3
- SuppTable4final.xlsx
Supplementary Table 4
- SuppTable5final.xlsx
Supplementary Table 5
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CURRENT STATUS: Under Review
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Posted 13 Jan, 2021
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Received 10 Jan, 2021
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On 03 Jan, 2021
Reviewers invited
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On 26 Dec, 2020
Editor invited
On 26 Dec, 2020
No comments provided
Review #2 received
Received 19 Nov, 2020
Editorial decision: Major revision
On 19 Nov, 2020
Reviewer #2 agreed
On 10 Oct, 2020
Review #1 received
Received 13 Jul, 2020
Reviewer #1 agreed
On 26 Jun, 2020
Reviewers invited
Invitations sent on 22 Jun, 2020
Editor assigned
On 17 Apr, 2020
First submitted
On 16 Apr, 2020
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On 16 Apr, 2020
Editor invited
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This preprint is under consideration at Biotechnology for Biofuels. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Accurate Determination of Genotypic Variance of Cell Wall Characteristics of a Populus trichocarpa Pedigree Using High-Throughput Pyrolysis-Molecular Beam Mass Spectrometry
Anne Elizabeth Harman-Ware
National Renewable Energy Laboratory
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7927-9424
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 13 Jan, 2021
No community comments so far
Review #1 received
Received 10 Jan, 2021
Reviewer #1 agreed
On 03 Jan, 2021
Reviewers invited
Invitations sent on 27 Dec, 2020
Editor assigned
On 26 Dec, 2020
Submission checks complete
On 26 Dec, 2020
Editor invited
On 26 Dec, 2020
No comments provided
Review #2 received
Received 19 Nov, 2020
Editorial decision: Major revision
On 19 Nov, 2020
Reviewer #2 agreed
On 10 Oct, 2020
Review #1 received
Received 13 Jul, 2020
Reviewer #1 agreed
On 26 Jun, 2020
Reviewers invited
Invitations sent on 22 Jun, 2020
Editor assigned
On 17 Apr, 2020
First submitted
On 16 Apr, 2020
Submission checks complete
On 16 Apr, 2020
Editor invited
On 16 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer composition. Instrumental and microspatial environmental variability were observed in the spectral features and corrected to reveal underlying genetic variance of biomass composition.
Results Lignin-derived ions (including m/z 124, 154, 168, 194, 210 and others) were highly impacted by microspatial environmental variation which demonstrates phenotypic plasticity of lignin composition in Populus trichocarpa biomass. Broad-sense heritability of lignin composition after correcting for microspatial and instrumental variation was determined to be H2 = 0.56 based on py-MBMS based on ions known to derive from lignin. Heritability of lignin monomeric syringyl/guaiacyl ratio (S/G) was H2 = 0.81. Broad-sense heritability was also high (up to H2 = 0.79) for ions derived from other components of the biomass including phenolics (e.g., salicylates) and C5 sugars (e.g., xylose). Lignin and phenolic ion abundances were primarily driven by maternal effects, and paternal effects were either similar or stronger for the most heritable carbohydrate-derived ions.
Conclusions We have shown that many biopolymer-derived ions from py-MBMS show substantial phenotypic plasticity in response to microenvironmental variation in plantations. Nevertheless, broad-sense heritability for biomass composition can be quite high after correcting for spatial environmental variation. This work outlines the importance in accounting for instrumental and microspatial environmental variation in biomass composition data for applications in heritability measurements and genomic selection for breeding poplar for renewable fuels and materials.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6