The first bovine parechovirus (Bo_ParV) was reported in 2021 and only two nearly complete genomes of Bo_ParV were available. In this study, we detected Bo_ParVs in 10 out of 158 bovine fecal samples using real-time RT-PCR and Bo_ParVs were isolated from three of these samples using MA104 cells. Analysis of the P1 region revealed that Bo_ParVs shared high pairwise amino acid sequence identities (≥95.7%), suggesting antigenic similarity among Bo_ParVs, whereas nucleotide sequence identities were diverse (≥84.8%). A recombination break point was identified in the 2B region, which may influence the evolution of this virus.