This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress.
Xiang-Cai Meng
Heilongjiang University of Chinese Medicine
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Plant have to face the more environmental stress than animal which can dodge unfavourable circumstances by moving about, it is inevitable that the reactive oxygen species(ROS), as a product of stress, are massive generated. Excessive ROS with a powerful oxidizability under stress could do harm to the protein, including enzymes. So it is impossible to eliminated too much ROS only by antioxidant enzymes. Plant have evolved a secondary metabolism as peculiar additional pathway, but the biosynthesis of secondary metabolites would be extremely costly due to the consumption of much material and energy during suitable condition, secondary metabolism are augmented only after the stress befall, meaning the secondary metabolites vary according to the ecological environment.
Results: Using UHPLC -ESI-Q-TOF-MS/MS analysis, a total of 24 differential compounts in root of Scutellaria baicalensis were identified between the drought and suitable condition. Based on Results of t-test analysis（P＜0.05）between various groups, ions whose VIP value ≧2, the most significant differential chemical markers of the drought condition were citric acid, shikimic acid, baicalin, wogonoside, baicalein, wogonin, 3,5,7,2',6'-pentahydroxyflavanone, 5,2',6'-trihydroxy-7,8 -dimethoxyflavone, chrysin, eriodictyol, 5,8-dihydroxy-6,7-dimethoxy flavone, highlighting that most of them were free flavonoids with many phenolic hydroxyl group of flavonoids, with a characteristic of higher avtivities.
Conclusions: The diversity of secondary metabolites plays a crucial role, S. baicalensis modified the ability to eliminate ROS and matained the equilibrium of ROS through the biosynthesis and convertion between these flavonoid which contain many compounts, like an intricate buffer solution.
Keywords
Scutellaria baicalensis, metabolomics, reactive oxygen species, antioxidant enzymes, secondary metabolite
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 2
Posted 23 May, 2020
No community comments so far
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Research article
Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress.
Xiang-Cai Meng
Heilongjiang University of Chinese Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 2
Posted 23 May, 2020
No community comments so far
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Plant have to face the more environmental stress than animal which can dodge unfavourable circumstances by moving about, it is inevitable that the reactive oxygen species(ROS), as a product of stress, are massive generated. Excessive ROS with a powerful oxidizability under stress could do harm to the protein, including enzymes. So it is impossible to eliminated too much ROS only by antioxidant enzymes. Plant have evolved a secondary metabolism as peculiar additional pathway, but the biosynthesis of secondary metabolites would be extremely costly due to the consumption of much material and energy during suitable condition, secondary metabolism are augmented only after the stress befall, meaning the secondary metabolites vary according to the ecological environment.
Results: Using UHPLC -ESI-Q-TOF-MS/MS analysis, a total of 24 differential compounts in root of Scutellaria baicalensis were identified between the drought and suitable condition. Based on Results of t-test analysis（P＜0.05）between various groups, ions whose VIP value ≧2, the most significant differential chemical markers of the drought condition were citric acid, shikimic acid, baicalin, wogonoside, baicalein, wogonin, 3,5,7,2',6'-pentahydroxyflavanone, 5,2',6'-trihydroxy-7,8 -dimethoxyflavone, chrysin, eriodictyol, 5,8-dihydroxy-6,7-dimethoxy flavone, highlighting that most of them were free flavonoids with many phenolic hydroxyl group of flavonoids, with a characteristic of higher avtivities.
Conclusions: The diversity of secondary metabolites plays a crucial role, S. baicalensis modified the ability to eliminate ROS and matained the equilibrium of ROS through the biosynthesis and convertion between these flavonoid which contain many compounts, like an intricate buffer solution.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7