1 Laing, R. A., Sanstrom, M. M., Berrospi, A. R. & Leibowitz, H. M. Changes in the corneal endothelium as a function of age. Exp Eye Res 22, 587-594 (1976).
2 Edelhauser, H. F. The resiliency of the corneal endothelium to refractive and intraocular surgery. Cornea 19, 263-273, doi:10.1097/00003226-200005000-00002 (2000).
3 Tuft, S. J. & Coster, D. J. The corneal endothelium. Eye (Lond) 4 ( Pt 3), 389-424, doi:10.1038/eye.1990.53 (1990).
4 Tan, D. T., Dart, J. K., Holland, E. J. & Kinoshita, S. Corneal transplantation. Lancet 379, 1749-1761, doi:10.1016/S0140-6736(12)60437-1 (2012).
5 Kinoshita, S. et al. Injection of Cultured Cells with a ROCK Inhibitor for Bullous Keratopathy. N Engl J Med 378, 995-1003, doi:10.1056/NEJMoa1712770 (2018).
6 Bourne, W. M. Cellular changes in transplanted human corneas. Cornea 20, 560-569, doi:10.1097/00003226-200108000-00002 (2001).
7 Fuest, M., Yam, G. H., Peh, G. S. & Mehta, J. S. Advances in corneal cell therapy. Regen Med 11, 601-615, doi:10.2217/rme-2016-0054 (2016).
8 Gauthier, A. S. et al. Very early endothelial cell loss after penetrating keratoplasty with organ-cultured corneas. Br J Ophthalmol 101, 1113-1118, doi:10.1136/bjophthalmol-2016-309615 (2017).
9 Monnereau, C. et al. Multicenter study of descemet membrane endothelial keratoplasty: first case series of 18 surgeons. JAMA Ophthalmol 132, 1192-1198, doi:10.1001/jamaophthalmol.2014.1710 (2014).
10 Bhogal, M., Balda, M. S., Matter, K. & Allan, B. D. Global cell-by-cell evaluation of endothelial viability after two methods of graft preparation in Descemet membrane endothelial keratoplasty. Br J Ophthalmol 100, 572-578, doi:10.1136/bjophthalmol-2015-307534 (2016).
11 Bhogal, M., Matter, K., Balda, M. S. & Allan, B. D. Organ culture storage of pre-prepared corneal donor material for Descemet's membrane endothelial keratoplasty. Br J Ophthalmol 100, 1576-1583, doi:10.1136/bjophthalmol-2016-308855 (2016).
12 Bhogal, M. et al. Real-time assessment of corneal endothelial cell damage following graft preparation and donor insertion for DMEK. PLoS One 12, e0184824, doi:10.1371/journal.pone.0184824 (2017).
13 Feng, M. T., Price, M. O., Miller, J. M. & Price, F. W., Jr. Air reinjection and endothelial cell density in Descemet membrane endothelial keratoplasty: five-year follow-up. J Cataract Refract Surg 40, 1116-1121, doi:10.1016/j.jcrs.2014.04.023 (2014).
14 Landry, H. et al. Corneal endothelial toxicity of air and SF6. Invest Ophthalmol Vis Sci 52, 2279-2286, doi:10.1167/iovs.10-6187 (2011).
15 Mitry, D. et al. Descemet stripping automated endothelial keratoplasty after failed penetrating keratoplasty: survival, rejection risk, and visual outcome. JAMA Ophthalmol 132, 742-749, doi:10.1001/jamaophthalmol.2014.352 (2014).
16 Maurino, V. & Aiello, F. Glaucoma risks in advanced corneal surgery. Prog Brain Res 221, 271-295, doi:10.1016/bs.pbr.2015.06.009 (2015).
17 Bhogal, M., Lwin, C. N., Seah, X. Y., Peh, G. & Mehta, J. S. Allogeneic Descemet's Membrane Transplantation Enhances Corneal Endothelial Monolayer Formation and Restores Functional Integrity Following Descemet's Stripping. Invest Ophthalmol Vis Sci 58, 4249-4260, doi:10.1167/iovs.17-22106 (2017).
18 Liu, Y. C., Lwin, N. C., Chan, N. S. & Mehta, J. S. Use of anterior segment optical coherence tomography to predict corneal graft rejection in small animal models. Invest Ophthalmol Vis Sci 55, 6736-6741, doi:10.1167/iovs.14-14475 (2014).
19 Rio-Cristobal, A. & Martin, R. Corneal assessment technologies: current status. Surv Ophthalmol 59, 599-614, doi:10.1016/j.survophthal.2014.05.001 (2014).
20 Ang, M. et al. Evaluation of a Micro-Optical Coherence Tomography for the Corneal Endothelium in an Animal Model. Sci Rep 6, 29769, doi:10.1038/srep29769 (2016).
21 McCarey, B. E., Edelhauser, H. F. & Lynn, M. J. Review of corneal endothelial specular microscopy for FDA clinical trials of refractive procedures, surgical devices, and new intraocular drugs and solutions. Cornea 27, 1-16, doi:10.1097/ICO.0b013e31815892da (2008).
22 Raecker, M. E., McLaren, J. W., Kittleson, K. M. & Patel, S. V. Endothelial image quality after descemet stripping with endothelial keratoplasty: a comparison of three microscopy techniques. Eye Contact Lens 37, 6-10, doi:10.1097/ICL.0b013e318203dc19 (2011).
23 Tanaka, H. et al. Panoramic view of human corneal endothelial cell layer observed by a prototype slit-scanning wide-field contact specular microscope. Br J Ophthalmol 101, 655-659, doi:10.1136/bjophthalmol-2016-308893 (2017).
24 Gasser, L., Daniel, M., Reinhard, T. & Bohringer, D. Long-term tracking of the central corneal endothelial mosaic. PLoS One 9, e88603, doi:10.1371/journal.pone.0088603 (2014).
25 Ong, A. P. et al. Post-surgical versus post-intravitreal injection endophthalmitis: changing patterns in causative flora. Clin Exp Ophthalmol 47, 57-62, doi:10.1111/ceo.13345 (2019).
26 Bahn, C. F. et al. Penetrating keratoplasty in the cat. A clinically applicable model. Ophthalmology 89, 687-699, doi:10.1016/s0161-6420(82)34750-8 (1982).
27 Haydari, M. N. et al. A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy. Invest Ophthalmol Vis Sci 53, 6343-6354, doi:10.1167/iovs.12-9708 (2012).
28 Genicio, N., Gallo Paramo, J. & Shortt, A. J. Quantum dot labeling and tracking of cultured limbal epithelial cell transplants in vitro. Invest Ophthalmol Vis Sci 56, 3051-3059, doi:10.1167/iovs.14-15973 (2015).
29 Hoppenreijs, V. P., Pels, E., Vrensen, G. F. & Treffers, W. F. Corneal endothelium and growth factors. Surv Ophthalmol 41, 155-164, doi:10.1016/s0039-6257(96)80005-1 (1996).
30 Nakano, Y. et al. Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo. Invest Ophthalmol Vis Sci 49, 93-104, doi:10.1167/iovs.07-0255 (2008).
31 Meekins, L. C. et al. Corneal Endothelial Cell Migration and Proliferation Enhanced by Rho Kinase (ROCK) Inhibitors in In Vitro and In Vivo Models. Invest Ophthalmol Vis Sci 57, 6731-6738, doi:10.1167/iovs.16-20414 (2016).
32 Koizumi, N., Okumura, N. & Kinoshita, S. Development of new therapeutic modalities for corneal endothelial disease focused on the proliferation of corneal endothelial cells using animal models. Experimental Eye Research 95, 60-67, doi:10.1016/j.exer.2011.10.014 (2012).
33 Qian, Y. & Dana, M. R. Molecular mechanisms of immunity in corneal allotransplantation and xenotransplantation. Expert Rev Mol Med 3, 1-21, doi:10.1017/S1462399401003246 (2001).
34 Ross, J. R., Howell, D. N. & Sanfilippo, F. P. Characteristics of corneal xenograft rejection in a discordant species combination. Invest Ophthalmol Vis Sci 34, 2469-2476 (1993).
35 Koudouna, E. et al. Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model. Invest Ophthalmol Vis Sci 58, 242-251, doi:10.1167/iovs.16-20019 (2017).
36 Hitani, K. et al. Transplantation of a sheet of human corneal endothelial cell in a rabbit model. Mol Vis 14, 1-9 (2008).
37 Koizumi, N. et al. Cultivated corneal endothelial cell sheet transplantation in a primate model. Invest Ophthalmol Vis Sci 48, 4519-4526, doi:10.1167/iovs.07-0567 (2007).
38 Flynn, T. H. et al. Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection. Br J Ophthalmol 94, 368-371, doi:10.1136/bjo.2009.160671 (2010).
39 Chauhan, S. K., Jurkunas, U., Funaki, T., Dastjerdi, M. & Dana, R. Quantification of allospecific and nonspecific corneal endothelial cell damage after corneal transplantation. Eye (Lond) 29, 136-144, doi:10.1038/eye.2014.248 (2015).
40 Merian, J., Gravier, J., Navarro, F. & Texier, I. Fluorescent nanoprobes dedicated to in vivo imaging: from preclinical validations to clinical translation. Molecules 17, 5564-5591, doi:10.3390/molecules17055564 (2012).
41 Vahrmeijer, A. L., Hutteman, M., van der Vorst, J. R., van de Velde, C. J. & Frangioni, J. V. Image-guided cancer surgery using near-infrared fluorescence. Nat Rev Clin Oncol 10, 507-518, doi:10.1038/nrclinonc.2013.123 (2013).
42 Cordeiro, M. F. et al. Real-time imaging of single neuronal cell apoptosis in patients with glaucoma. Brain 140, 1757-1767, doi:10.1093/brain/awx088 (2017).
43 Pels, E., Beele, H. & Claerhout, I. Eye bank issues: II. Preservation techniques: warm versus cold storage. Int Ophthalmol 28, 155-163, doi:10.1007/s10792-007-9086-1 (2008).
44 Vinegoni, C. et al. Real-time high dynamic range laser scanning microscopy. Nat Commun 7, 11077, doi:10.1038/ncomms11077 (2016).
45 Hertsenberg, A. J. et al. Corneal stromal stem cells reduce corneal scarring by mediating neutrophil infiltration after wounding. PLoS One 12, e0171712, doi:10.1371/journal.pone.0171712 (2017).
46 Percie du Sert, N. et al. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLoS Biol 18, e3000410, doi:10.1371/journal.pbio.3000410 (2020).
47 Peh, G. S. L. et al. Propagation of Human Corneal Endothelial Cells: A Novel Dual Media Approach. Cell Transplantation 24, 287-304, doi:10.3727/096368913x675719 (2015).
48 Bhogal, M., Maurino, V. & Allan, B. D. Use of a single peripheral triangular mark to ensure correct graft orientation in Descemet membrane endothelial keratoplasty. J Cataract Refract Surg 41, 2022-2024, doi:10.1016/j.jcrs.2015.08.005 (2015).
49 Bhogal, M. S. & Allan, B. D. Graft profile and thickness as a function of cut transition speed in Descemet-stripping automated endothelial keratoplasty. J Cataract Refract Surg 38, 690-695, doi:10.1016/j.jcrs.2011.09.046 (2012).