See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Guo-Hua Liu
Hunan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8434-899X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Fragmented mitochondrial (mt) genomes and extensive mt gene rearrangements have been frequently reported from parasitic lice (Insecta: Phthiraptera). However, relatively little is available about the mt genomes from the family Philopteridae that is the most species-rich family within the suborder Ischnocera.
Methods: Herein, we use next-generation sequencing to decode the mt genome sequences of Falcolipeurus suturalis and compared it with the mt genome sequences of F. quadripustulatus. Phylogenetic relationship of the concatenated amino acid sequence data for 13 protein-coding genes of the two Falcolipeurus lice and selected members of the family Philopteridae was evaluated using Bayesian inference (BI).
Results: The complete mt genome of F. suturalis is a circular double-stranded DNA molecule of 16,659 bp, and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, as well as three putative non-coding regions. The gene order in F. suturalis mt genome was rearranged compared with that of F. quadripustulatus, and they were radical different from other louse species and the ancestral insect. Phylogenetic analyses revealed that the clear genetic distinctiveness between F. suturalis and F. quadripustulatus (Bayesian posterior probabilities=1.0), and the genus Falcolipeurus is more closely related to the genus Ibidoecus than to other genera (Bayesian posterior probabilities=1.0).
Conclusions: These novel datasets will help to better understand the gene rearrangements and phylogenetic position of Falcolipeurus and provide useful genetic markers for systematics and phylogenetic studies of bird lice.
Keywords
Bird lice, Mitochondrial genome, Gene rearrangement, Phylogenetic analyses
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Fig. S1: PCR amplicons from the mitochondrial genome of Falcolipeurus suturalis. Amplicons generated with the F. suturalis primers. M: DL8000 DNA marker, 1: Validation_01, 2: Validation_02, 3: Validation_03, 4: Validation_04, 5: Validation_05, 6: Negative control.
- TableS1.docx
Table S1 Primers used for assembly validation.
- GraphicalAbstract.tif
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 24 Feb, 2021
No community comments so far
Review #3 received
Received 07 Mar, 2021
Editorial decision: Major Revision
On 07 Mar, 2021
Review #2 received
Received 28 Feb, 2021
Review #1 received
Received 25 Feb, 2021
Reviewer #3 agreed
On 23 Feb, 2021
Reviewer #2 agreed
On 22 Feb, 2021
Reviews received
Received 21 Feb, 2021
Reviewer #1 agreed
On 21 Feb, 2021
Reviewers invited
Invitations sent on 21 Feb, 2021
Editor assigned
On 16 Feb, 2021
Editor invited
On 16 Feb, 2021
Submission checks complete
On 16 Feb, 2021
First submitted
On 16 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Guo-Hua Liu
Hunan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8434-899X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 24 Feb, 2021
No community comments so far
Review #3 received
Received 07 Mar, 2021
Editorial decision: Major Revision
On 07 Mar, 2021
Review #2 received
Received 28 Feb, 2021
Review #1 received
Received 25 Feb, 2021
Reviewer #3 agreed
On 23 Feb, 2021
Reviewer #2 agreed
On 22 Feb, 2021
Reviews received
Received 21 Feb, 2021
Reviewer #1 agreed
On 21 Feb, 2021
Reviewers invited
Invitations sent on 21 Feb, 2021
Editor assigned
On 16 Feb, 2021
Editor invited
On 16 Feb, 2021
Submission checks complete
On 16 Feb, 2021
First submitted
On 16 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background: Fragmented mitochondrial (mt) genomes and extensive mt gene rearrangements have been frequently reported from parasitic lice (Insecta: Phthiraptera). However, relatively little is available about the mt genomes from the family Philopteridae that is the most species-rich family within the suborder Ischnocera.
Methods: Herein, we use next-generation sequencing to decode the mt genome sequences of Falcolipeurus suturalis and compared it with the mt genome sequences of F. quadripustulatus. Phylogenetic relationship of the concatenated amino acid sequence data for 13 protein-coding genes of the two Falcolipeurus lice and selected members of the family Philopteridae was evaluated using Bayesian inference (BI).
Results: The complete mt genome of F. suturalis is a circular double-stranded DNA molecule of 16,659 bp, and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, as well as three putative non-coding regions. The gene order in F. suturalis mt genome was rearranged compared with that of F. quadripustulatus, and they were radical different from other louse species and the ancestral insect. Phylogenetic analyses revealed that the clear genetic distinctiveness between F. suturalis and F. quadripustulatus (Bayesian posterior probabilities=1.0), and the genus Falcolipeurus is more closely related to the genus Ibidoecus than to other genera (Bayesian posterior probabilities=1.0).
Conclusions: These novel datasets will help to better understand the gene rearrangements and phylogenetic position of Falcolipeurus and provide useful genetic markers for systematics and phylogenetic studies of bird lice.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Fig. S1: PCR amplicons from the mitochondrial genome of Falcolipeurus suturalis. Amplicons generated with the F. suturalis primers. M: DL8000 DNA marker, 1: Validation_01, 2: Validation_02, 3: Validation_03, 4: Validation_04, 5: Validation_05, 6: Negative control.
- TableS1.docx
Table S1 Primers used for assembly validation.
- GraphicalAbstract.tif
Loading...