This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Hangxiao Zhang
Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Betula fujianensis (B. fujianensis), a new Betulaceae species reported in 2008, is endemic and endangered in the Fujian province of China. However, little is known about it, and no special protection measures have been taken. So it is urgent to take some activities for its well conservation.
Results: In this study, we systematically described its morphological characteristics, developed a cultivation method, and were the first to report its whole chloroplast genome sequence. We conducted a comparative analysis using its synonymous species (B. luminifera) and identified its distinct characteristic, especially in bark and leaves. It has serrated leaves covered with glandular punctation and short and rare pubescences, and its bark gradually changes with age from smooth to exfoliate. We also observed that its chlorophyll content substantially changes with age. Furthermore, we also sequenced B. luminifera’s chloroplast genome sequence, compared it with that of B. fujianensis, and found that gene rpl16 is absent in B. fujianensis. Phylogenetic analysis also showed that B. fujianensis is relatively close to B. luminifera.
Conclusions: These observations and chloroplast genome sequence results provide more information about B. fujianensis. The comparative analysis with B. luminifera will provide some clues to different environmental adaptation. The cultivation method make further scientific researches possible, contribute to its good conservation and promote its utilization in afforestation and industrial application in the future.
Keywords
Chloroplast genome, Betula, Cultivation, Betula fujianensis
Figure 1
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This preprint is available for download as a PDF.
This is a list of supplementary files associated with this preprint. Click to download.
- Figs1.pdf
Fig. S1 Chloroplast genome map of B. fujianensis and B. luminifera.
- TableS1SSRsofchloroplastgenomeofBetulaluminifera.xlsx
Table S1 SSRs of chloroplast genome of B. luminifera.
- TableS2SSRsofchloroplastgenomeofBetulafujianensis..xlsx
Table S2 SSRs of chloroplast genome of Betula fujianensis.
- TableS3ComparisonofSSRs.xlsx
Table S3 Comparison of SSRs in Betula fujianensis and B. luminifera.
- TableS4RepeatedsequencesinBetulaluminifera.xlsx
Table S4 Analysis of repeated sequences in B. luminifera.
- TableS5RepeatedsequencesinB.fujianensis.xlsx
Table S5 Analysis of repeated sequences in B. fujianensis.
- TableS7RNAeditingsites.xlsx
Table S7 RNA editing sites of Betula fujianensis and B. luminifera.
- TableS6SPADvalueandchlorophyllcontent.xls
Table S6 SPAD value and chlorophyll content of Betula fujianensis and B. luminifera.
- Table1CleanData.xlsx
- Table2listofgenespresentinthechloroplastgenomes.xlsx
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Hangxiao Zhang
Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Forestry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Betula fujianensis (B. fujianensis), a new Betulaceae species reported in 2008, is endemic and endangered in the Fujian province of China. However, little is known about it, and no special protection measures have been taken. So it is urgent to take some activities for its well conservation.
Results: In this study, we systematically described its morphological characteristics, developed a cultivation method, and were the first to report its whole chloroplast genome sequence. We conducted a comparative analysis using its synonymous species (B. luminifera) and identified its distinct characteristic, especially in bark and leaves. It has serrated leaves covered with glandular punctation and short and rare pubescences, and its bark gradually changes with age from smooth to exfoliate. We also observed that its chlorophyll content substantially changes with age. Furthermore, we also sequenced B. luminifera’s chloroplast genome sequence, compared it with that of B. fujianensis, and found that gene rpl16 is absent in B. fujianensis. Phylogenetic analysis also showed that B. fujianensis is relatively close to B. luminifera.
Conclusions: These observations and chloroplast genome sequence results provide more information about B. fujianensis. The comparative analysis with B. luminifera will provide some clues to different environmental adaptation. The cultivation method make further scientific researches possible, contribute to its good conservation and promote its utilization in afforestation and industrial application in the future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This preprint is available for download as a PDF.
This is a list of supplementary files associated with this preprint. Click to download.
- Figs1.pdf
Fig. S1 Chloroplast genome map of B. fujianensis and B. luminifera.
- TableS1SSRsofchloroplastgenomeofBetulaluminifera.xlsx
Table S1 SSRs of chloroplast genome of B. luminifera.
- TableS2SSRsofchloroplastgenomeofBetulafujianensis..xlsx
Table S2 SSRs of chloroplast genome of Betula fujianensis.
- TableS3ComparisonofSSRs.xlsx
Table S3 Comparison of SSRs in Betula fujianensis and B. luminifera.
- TableS4RepeatedsequencesinBetulaluminifera.xlsx
Table S4 Analysis of repeated sequences in B. luminifera.
- TableS5RepeatedsequencesinB.fujianensis.xlsx
Table S5 Analysis of repeated sequences in B. fujianensis.
- TableS7RNAeditingsites.xlsx
Table S7 RNA editing sites of Betula fujianensis and B. luminifera.
- TableS6SPADvalueandchlorophyllcontent.xls
Table S6 SPAD value and chlorophyll content of Betula fujianensis and B. luminifera.
- Table1CleanData.xlsx
- Table2listofgenespresentinthechloroplastgenomes.xlsx
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