Cell extrusion is a universal mode of cell removal from tissues, and it plays an important role in regulating cell numbers and eliminating unwanted cells, such as apoptotic, unfit, or cancerous. During this process, cells delaminate from the cell layer, however, the underlying mechanisms remain to be elucidated. Here, we report a conserved execution mechanism of cell extrusion. We found extracellular vesicle (EV) formation in extruding cells at a site opposite to the extrusion direction. Particularly, we found that a lipid-scramblase‒mediated local exposure of phosphatidylserine is responsible for EV formation and is crucial for executing cell extrusion, while inhibition of this process disrupted prompt cell delamination and tissue homeostasis. Furthermore, we revealed that the EV formation is governed by the mechanism in microvesicle formation, while the EVs have some hallmarks of apoptotic body. Finally, we illustrated the role of EV formation as promoting the neighboring cells’ invasion resulting in the execution of cell extrusion by experimental and mathematical modeling analysis. Taken together, this study provides the insights that membrane dynamics plays a crucial role behind the cell exit from the tissue by connecting the actions of extruding cell and the neighboring cells.