Proteomic investigation of protein adsorption to cerebral microdialysis membranes in surgically treated intracerebral hemorrhage patients - a pilot study
Background: Cerebral microdialysis (CMD) is a minimally invasive technique for sampling the interstitial fluid in human brain tissue. CMD allows monitoring the metabolic state of tissue, as well as sampling macromolecules such as proteins and peptides. Recovery of proteins or peptides can be hampered by their adsorption to the CMD membrane as has been previously shown in-vitro, however, protein adsorption to CMD membranes has not been characterized following implantation in human brain tissue.
Methods: In this paper, we describe the pattern of proteins adsorbed to CMD membranes compared to that of the microdialysate and of cerebrospinal fluid (CSF). We retrieved CMD membranes from three surgically treated intracerebral hemorrhage (ICH) patients, and analyzed protein adsorption to the membranes using two-dimensional gel electrophoresis (2-DE) in combination with nano-liquid mass spectrometry. We compared the proteome profile of three compartments; the CMD membrane, the microdialysate and ventricular CSF collected at time of CMD removal.
Results: We found unique protein patterns in the molecular weight range of 10-35 kDa for each of the three compartments.
Conclusion: This study highlights the importance of analyzing the membranes in addition to the microdialysate when using CMD to sample proteins for biomarker investigation.
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Posted 22 Jun, 2020
On 25 Jul, 2020
Received 22 Jun, 2020
On 22 Jun, 2020
On 21 Jun, 2020
On 18 Jun, 2020
Invitations sent on 18 Jun, 2020
On 17 Jun, 2020
On 17 Jun, 2020
Received 21 May, 2020
On 21 May, 2020
Received 19 May, 2020
On 10 May, 2020
Invitations sent on 07 May, 2020
On 07 May, 2020
On 29 Apr, 2020
On 28 Apr, 2020
On 28 Apr, 2020
On 28 Apr, 2020
Proteomic investigation of protein adsorption to cerebral microdialysis membranes in surgically treated intracerebral hemorrhage patients - a pilot study
Posted 22 Jun, 2020
On 25 Jul, 2020
Received 22 Jun, 2020
On 22 Jun, 2020
On 21 Jun, 2020
On 18 Jun, 2020
Invitations sent on 18 Jun, 2020
On 17 Jun, 2020
On 17 Jun, 2020
Received 21 May, 2020
On 21 May, 2020
Received 19 May, 2020
On 10 May, 2020
Invitations sent on 07 May, 2020
On 07 May, 2020
On 29 Apr, 2020
On 28 Apr, 2020
On 28 Apr, 2020
On 28 Apr, 2020
Background: Cerebral microdialysis (CMD) is a minimally invasive technique for sampling the interstitial fluid in human brain tissue. CMD allows monitoring the metabolic state of tissue, as well as sampling macromolecules such as proteins and peptides. Recovery of proteins or peptides can be hampered by their adsorption to the CMD membrane as has been previously shown in-vitro, however, protein adsorption to CMD membranes has not been characterized following implantation in human brain tissue.
Methods: In this paper, we describe the pattern of proteins adsorbed to CMD membranes compared to that of the microdialysate and of cerebrospinal fluid (CSF). We retrieved CMD membranes from three surgically treated intracerebral hemorrhage (ICH) patients, and analyzed protein adsorption to the membranes using two-dimensional gel electrophoresis (2-DE) in combination with nano-liquid mass spectrometry. We compared the proteome profile of three compartments; the CMD membrane, the microdialysate and ventricular CSF collected at time of CMD removal.
Results: We found unique protein patterns in the molecular weight range of 10-35 kDa for each of the three compartments.
Conclusion: This study highlights the importance of analyzing the membranes in addition to the microdialysate when using CMD to sample proteins for biomarker investigation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5