See the published version of this preprint at Proteome Science.
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Research
Lovisa Tobieson
Universitetssjukhuset i Linkoping Neurokirurgiska kliniken
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2284-846X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Cerebral microdialysis (CMD) is a minimally invasive technique for sampling the interstitial fluid in human brain tissue. CMD allows monitoring the metabolic state of tissue, as well as sampling macromolecules such as proteins and peptides. Recovery of proteins or peptides can be hampered by their adsorption to the CMD membrane as has been previously shown in-vitro, however, protein adsorption to CMD membranes has not been characterized following implantation in human brain tissue.
Methods: In this paper, we describe the pattern of proteins adsorbed to CMD membranes compared to that of the microdialysate and of cerebrospinal fluid (CSF). We retrieved CMD membranes from three surgically treated intracerebral hemorrhage (ICH) patients, and analyzed protein adsorption to the membranes using two-dimensional gel electrophoresis (2-DE) in combination with nano-liquid mass spectrometry. We compared the proteome profile of three compartments; the CMD membrane, the microdialysate and ventricular CSF collected at time of CMD removal.
Results: We found unique protein patterns in the molecular weight range of 10-35 kDa for each of the three compartments.
Conclusion: This study highlights the importance of analyzing the membranes in addition to the microdialysate when using CMD to sample proteins for biomarker investigation.
Keywords
Cerebral microdialysis, intracerebral hemorrhage, proteomics, biomarker, protein adsorption, relative recovery
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View the published article at Proteome Science.
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Posted 22 Jun, 2020
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Review #1 received
Received 22 Jun, 2020
Editorial decision: Accept
On 22 Jun, 2020
Reviewer #1 agreed
On 21 Jun, 2020
Editor assigned
On 18 Jun, 2020
Reviewers invited
Invitations sent on 18 Jun, 2020
Submission checks complete
On 17 Jun, 2020
Editor invited
On 17 Jun, 2020
No comments provided
Review #2 received
Received 21 May, 2020
Editorial decision: Major revision
On 21 May, 2020
Review #1 received
Received 19 May, 2020
Reviewer #2 agreed
On 10 May, 2020
Reviewers invited
Invitations sent on 07 May, 2020
Reviewer #1 agreed
On 07 May, 2020
Editor assigned
On 29 Apr, 2020
First submitted
On 28 Apr, 2020
Submission checks complete
On 28 Apr, 2020
Editor invited
On 28 Apr, 2020
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See the published version of this preprint at Proteome Science.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Lovisa Tobieson
Universitetssjukhuset i Linkoping Neurokirurgiska kliniken
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2284-846X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Proteome Science.
Version 2
Posted 22 Jun, 2020
No community comments so far
Review #1 received
Received 22 Jun, 2020
Editorial decision: Accept
On 22 Jun, 2020
Reviewer #1 agreed
On 21 Jun, 2020
Editor assigned
On 18 Jun, 2020
Reviewers invited
Invitations sent on 18 Jun, 2020
Submission checks complete
On 17 Jun, 2020
Editor invited
On 17 Jun, 2020
No comments provided
Review #2 received
Received 21 May, 2020
Editorial decision: Major revision
On 21 May, 2020
Review #1 received
Received 19 May, 2020
Reviewer #2 agreed
On 10 May, 2020
Reviewers invited
Invitations sent on 07 May, 2020
Reviewer #1 agreed
On 07 May, 2020
Editor assigned
On 29 Apr, 2020
First submitted
On 28 Apr, 2020
Submission checks complete
On 28 Apr, 2020
Editor invited
On 28 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Proteome Science.
Background: Cerebral microdialysis (CMD) is a minimally invasive technique for sampling the interstitial fluid in human brain tissue. CMD allows monitoring the metabolic state of tissue, as well as sampling macromolecules such as proteins and peptides. Recovery of proteins or peptides can be hampered by their adsorption to the CMD membrane as has been previously shown in-vitro, however, protein adsorption to CMD membranes has not been characterized following implantation in human brain tissue.
Methods: In this paper, we describe the pattern of proteins adsorbed to CMD membranes compared to that of the microdialysate and of cerebrospinal fluid (CSF). We retrieved CMD membranes from three surgically treated intracerebral hemorrhage (ICH) patients, and analyzed protein adsorption to the membranes using two-dimensional gel electrophoresis (2-DE) in combination with nano-liquid mass spectrometry. We compared the proteome profile of three compartments; the CMD membrane, the microdialysate and ventricular CSF collected at time of CMD removal.
Results: We found unique protein patterns in the molecular weight range of 10-35 kDa for each of the three compartments.
Conclusion: This study highlights the importance of analyzing the membranes in addition to the microdialysate when using CMD to sample proteins for biomarker investigation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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