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Research
Xinsheng Yao
Zunyi Medical University
Corresponding Author
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The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mouse central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of some V, D, and J genes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5'D insertion, and 5'D trimming with age. The BCR H-CDR3 repertoire diversity of mouse bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.
Keywords
Analysis, heterogeneity of the BCR H-CDR3, repertoire, bone marrow and spleen, mice
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CURRENT STATUS: Under Review
Version 2
Posted 18 Nov, 2020
No community comments so far
Editorial decision: Minor Revision
On 25 Jan, 2021
Review #2 received
Received 24 Jan, 2021
Reviewer #2 agreed
On 28 Dec, 2020
Reviewers invited
Invitations sent on 10 Nov, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Review #1 received
Received 10 Nov, 2020
Editor assigned
On 09 Nov, 2020
Submission checks complete
On 09 Nov, 2020
Editor invited
On 09 Nov, 2020
No comments provided
Editorial decision: Major Revision
On 16 Sep, 2020
Review #1 received
Received 18 Jun, 2020
Review #2 received
Received 18 Jun, 2020
Reviewers invited
Invitations sent on 05 Jun, 2020
Reviewer #1 agreed
On 05 Jun, 2020
Reviewer #2 agreed
On 05 Jun, 2020
Editor assigned
On 02 Jun, 2020
Editor invited
On 01 Jun, 2020
Submission checks complete
On 22 May, 2020
First submitted
On 16 May, 2020
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This preprint is under consideration at Immunity & Ageing. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Xinsheng Yao
Zunyi Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 18 Nov, 2020
No community comments so far
Editorial decision: Minor Revision
On 25 Jan, 2021
Review #2 received
Received 24 Jan, 2021
Reviewer #2 agreed
On 28 Dec, 2020
Reviewers invited
Invitations sent on 10 Nov, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Review #1 received
Received 10 Nov, 2020
Editor assigned
On 09 Nov, 2020
Submission checks complete
On 09 Nov, 2020
Editor invited
On 09 Nov, 2020
No comments provided
Editorial decision: Major Revision
On 16 Sep, 2020
Review #1 received
Received 18 Jun, 2020
Review #2 received
Received 18 Jun, 2020
Reviewers invited
Invitations sent on 05 Jun, 2020
Reviewer #1 agreed
On 05 Jun, 2020
Reviewer #2 agreed
On 05 Jun, 2020
Editor assigned
On 02 Jun, 2020
Editor invited
On 01 Jun, 2020
Submission checks complete
On 22 May, 2020
First submitted
On 16 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mouse central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of some V, D, and J genes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5'D insertion, and 5'D trimming with age. The BCR H-CDR3 repertoire diversity of mouse bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.
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