Analysis of the heterogeneity of the BCR H-CDR3 repertoire in the bone marrow and spleen of 3-, 12-, and 20-month old mice
The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mouse central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of some V, D, and J genes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5'D insertion, and 5'D trimming with age. The BCR H-CDR3 repertoire diversity of mouse bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.
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Posted 18 Nov, 2020
On 25 Jan, 2021
Received 24 Jan, 2021
On 28 Dec, 2020
Invitations sent on 10 Nov, 2020
On 10 Nov, 2020
Received 10 Nov, 2020
On 09 Nov, 2020
On 09 Nov, 2020
On 09 Nov, 2020
On 16 Sep, 2020
Received 18 Jun, 2020
Received 18 Jun, 2020
Invitations sent on 05 Jun, 2020
On 05 Jun, 2020
On 05 Jun, 2020
On 02 Jun, 2020
On 01 Jun, 2020
On 22 May, 2020
On 16 May, 2020
Analysis of the heterogeneity of the BCR H-CDR3 repertoire in the bone marrow and spleen of 3-, 12-, and 20-month old mice
Posted 18 Nov, 2020
On 25 Jan, 2021
Received 24 Jan, 2021
On 28 Dec, 2020
Invitations sent on 10 Nov, 2020
On 10 Nov, 2020
Received 10 Nov, 2020
On 09 Nov, 2020
On 09 Nov, 2020
On 09 Nov, 2020
On 16 Sep, 2020
Received 18 Jun, 2020
Received 18 Jun, 2020
Invitations sent on 05 Jun, 2020
On 05 Jun, 2020
On 05 Jun, 2020
On 02 Jun, 2020
On 01 Jun, 2020
On 22 May, 2020
On 16 May, 2020
The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mouse central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of some V, D, and J genes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5'D insertion, and 5'D trimming with age. The BCR H-CDR3 repertoire diversity of mouse bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6