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Research
Kun Xu
Northwest A&F University: Northwest Agriculture and Forestry University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5714-9090
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: MicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs with a length of about 22 nucleotides and play critical roles in the regulation of posttranscriptional gene expression. miR-487b-3p has been recently identified as a key regulator in skeletal muscle growth and development. However, the function of miR-487b-3p on goat skeletal muscle remains to be investigated.
Results: In this study, we found that miR-487b-3p was widespread in goat different tissues, with a significantly higher expression in muscle, especially in lamb. The results demonstrated that the expression of miR-487b-3p was gradually up-regulated during myoblast proliferation. Then, miR-487b-3p knockout primary goat myoblast cells clones were obtained by using CRISPR/Cas9 system and our RPG surrogate reporter-based screening. Further investigation uncovered that the knockout of miR-487b-3p significantly accelerated primary goat myoblast proliferation, which was accompanied by up-regulation of cell cycle-related genes, such as PCNA, Cyclin E and CDK2. What’s more, we found that miR-487b-3p targets directly the 3'UTR of insulin receptor substrate 1 (IRS1) gene and IRS1 knockdown by siRNA was able to down-regulate the expression of the cell cycle-related genes.
Conclusions: Collectively, these founding demonstrated miR-487b-3p as a potent inhibitor of cell proliferation which functions by targeting IRS1 gene in primary goat myoblast.
Keywords
miR-487b-3p, CRISPR/Cas9, Primary goat myoblast, Proliferation
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This is a preprint. It has not completed peer review.
Research
Kun Xu
Northwest A&F University: Northwest Agriculture and Forestry University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5714-9090
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Mar, 2021
No community comments so far
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: MicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs with a length of about 22 nucleotides and play critical roles in the regulation of posttranscriptional gene expression. miR-487b-3p has been recently identified as a key regulator in skeletal muscle growth and development. However, the function of miR-487b-3p on goat skeletal muscle remains to be investigated.
Results: In this study, we found that miR-487b-3p was widespread in goat different tissues, with a significantly higher expression in muscle, especially in lamb. The results demonstrated that the expression of miR-487b-3p was gradually up-regulated during myoblast proliferation. Then, miR-487b-3p knockout primary goat myoblast cells clones were obtained by using CRISPR/Cas9 system and our RPG surrogate reporter-based screening. Further investigation uncovered that the knockout of miR-487b-3p significantly accelerated primary goat myoblast proliferation, which was accompanied by up-regulation of cell cycle-related genes, such as PCNA, Cyclin E and CDK2. What’s more, we found that miR-487b-3p targets directly the 3'UTR of insulin receptor substrate 1 (IRS1) gene and IRS1 knockdown by siRNA was able to down-regulate the expression of the cell cycle-related genes.
Conclusions: Collectively, these founding demonstrated miR-487b-3p as a potent inhibitor of cell proliferation which functions by targeting IRS1 gene in primary goat myoblast.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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