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Research article
Molecular marker assisted gene stacking for disease resistance and quality genes in the dwarf mutant of an elite common wheat cultivar Xiaoyan22
weijun zheng
Northwest A&F University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2416-8110
License:
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Background: Development of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs. Gene stacking is an efficient approach to achieve this target. In this study, we pyramided yellow rust resistance gene (Yr26), powdery mildew resistance gene (ML91260) and high-molecular-weight glutenin subunits Dx5+Dy10 into the dwarf mutant of an elite wheat cultivar, Xiaoyan22.
Results: Six pyramided wheat lines were obtained by molecular marker-assisted selection (MAS) and field evaluation of disease resistance. The desirable agronomic traits of pyramided lines, their identity with the original cultivar Xiaoyan22 except for plant height, tiller number and disease resistance, was achieved in this study. Meanwhile, the yield of pyramided lines is higher than Xiaoyan22 in the field test. In addition, analysis of flour quality indicated that the dough stability time of pyramided lines was longer than that of Xiaoyan22.
Conclusions: Six pyramided wheat lines with two disease resistance and high quality were achieved in this study. It is feasible to improve multiple agronomic traits simultaneously by rational application of MAS.
Keywords
Wheat, Marker-assisted selection, Powdery mildew resistance, Yellow rust resistance, Grain quality
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CURRENT STATUS: Under Review
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Posted 14 Apr, 2020
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Editor assigned
On 12 Apr, 2020
Editor invited
On 11 Apr, 2020
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On 08 Apr, 2020
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Editorial decision: Minor revision
On 06 Apr, 2020
Review #2 received
Received 04 Apr, 2020
Reviewer #2 agreed
On 16 Mar, 2020
Review #1 received
Received 11 Jan, 2020
Reviewer #1 agreed
On 18 Dec, 2019
Reviewers invited
Invitations sent on 17 Dec, 2019
Editor assigned
On 16 Dec, 2019
Submission checks complete
On 15 Dec, 2019
Editor invited
On 15 Dec, 2019
No comments provided
Review #2 received
Received 02 Dec, 2019
Editorial decision: Minor revision
On 02 Dec, 2019
Review #1 received
Received 29 Nov, 2019
Reviewer #2 agreed
On 14 Nov, 2019
Reviewer #1 agreed
On 08 Nov, 2019
Reviewers invited
Invitations sent on 05 Aug, 2019
Submission checks complete
On 23 Jul, 2019
First submitted
On 30 Jun, 2019
Editor assigned
On 30 Jun, 2019
Editor invited
On 29 Jun, 2019
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This preprint is under consideration at BMC Genomic Data. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Molecular marker assisted gene stacking for disease resistance and quality genes in the dwarf mutant of an elite common wheat cultivar Xiaoyan22
weijun zheng
Northwest A&F University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2416-8110
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 14 Apr, 2020
No community comments so far
Editor assigned
On 12 Apr, 2020
Editor invited
On 11 Apr, 2020
Submission checks complete
On 08 Apr, 2020
No comments provided
Editorial decision: Minor revision
On 06 Apr, 2020
Review #2 received
Received 04 Apr, 2020
Reviewer #2 agreed
On 16 Mar, 2020
Review #1 received
Received 11 Jan, 2020
Reviewer #1 agreed
On 18 Dec, 2019
Reviewers invited
Invitations sent on 17 Dec, 2019
Editor assigned
On 16 Dec, 2019
Submission checks complete
On 15 Dec, 2019
Editor invited
On 15 Dec, 2019
No comments provided
Review #2 received
Received 02 Dec, 2019
Editorial decision: Minor revision
On 02 Dec, 2019
Review #1 received
Received 29 Nov, 2019
Reviewer #2 agreed
On 14 Nov, 2019
Reviewer #1 agreed
On 08 Nov, 2019
Reviewers invited
Invitations sent on 05 Aug, 2019
Submission checks complete
On 23 Jul, 2019
First submitted
On 30 Jun, 2019
Editor assigned
On 30 Jun, 2019
Editor invited
On 29 Jun, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Development of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs. Gene stacking is an efficient approach to achieve this target. In this study, we pyramided yellow rust resistance gene (Yr26), powdery mildew resistance gene (ML91260) and high-molecular-weight glutenin subunits Dx5+Dy10 into the dwarf mutant of an elite wheat cultivar, Xiaoyan22.
Results: Six pyramided wheat lines were obtained by molecular marker-assisted selection (MAS) and field evaluation of disease resistance. The desirable agronomic traits of pyramided lines, their identity with the original cultivar Xiaoyan22 except for plant height, tiller number and disease resistance, was achieved in this study. Meanwhile, the yield of pyramided lines is higher than Xiaoyan22 in the field test. In addition, analysis of flour quality indicated that the dough stability time of pyramided lines was longer than that of Xiaoyan22.
Conclusions: Six pyramided wheat lines with two disease resistance and high quality were achieved in this study. It is feasible to improve multiple agronomic traits simultaneously by rational application of MAS.
Figure 1
Figure 2
Figure 3