SP1 induced Long non-coding RNA AGAP2-AS1
promotes cholangiocarcinoma proliferation via silencing of CDKN1A
Background: LncRNA can regulate gene at various levels such as apparent genetics, alternative splicing, and regulation of mRNA degradation. However, the molecular mechanism of LncRNA in cholangiocarcinoma is still unclear. This deserves further exploration.
Methods:We investigated the expression of AGAP2-AS1 in 32 CCA tissues and two CCA cell lines. We found a LncRNA AGAP2-AS1 which induced by SP1 has not been reported in CCA, and Knockdown and overexpression were used to investigate the biological role of AGAP2-AS1 in vitro. CHIP and RIP were performed to verify the putative targets of AGAP2-AS1.
Results:AGAP2-AS1 was significantly upregulated in CCA tumor tissues.SP1 induced AGAP2-AS1 plays an important role in tumorigenesis. AGAP2-AS1 knockdown significantly inhibited proliferation and caused apoptosis in CCA cells. In addition, we demonstrated that AGAP2-AS1 promotes the proliferation of CCA.
Conclusions: We conclude that the long non-coding RNA AGAP2-AS1 plays a role in promoting the proliferation of cholangiocarcinoma.
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Posted 17 Sep, 2020
Received 25 Sep, 2020
On 16 Sep, 2020
Invitations sent on 16 Sep, 2020
On 16 Sep, 2020
On 15 Sep, 2020
On 15 Sep, 2020
Received 19 Jun, 2020
On 19 Jun, 2020
On 15 Jun, 2020
Received 12 Jun, 2020
On 27 May, 2020
On 20 May, 2020
Invitations sent on 20 May, 2020
On 19 May, 2020
On 14 May, 2020
On 08 May, 2020
SP1 induced Long non-coding RNA AGAP2-AS1
promotes cholangiocarcinoma proliferation via silencing of CDKN1A
Posted 17 Sep, 2020
Received 25 Sep, 2020
On 16 Sep, 2020
Invitations sent on 16 Sep, 2020
On 16 Sep, 2020
On 15 Sep, 2020
On 15 Sep, 2020
Received 19 Jun, 2020
On 19 Jun, 2020
On 15 Jun, 2020
Received 12 Jun, 2020
On 27 May, 2020
On 20 May, 2020
Invitations sent on 20 May, 2020
On 19 May, 2020
On 14 May, 2020
On 08 May, 2020
Background: LncRNA can regulate gene at various levels such as apparent genetics, alternative splicing, and regulation of mRNA degradation. However, the molecular mechanism of LncRNA in cholangiocarcinoma is still unclear. This deserves further exploration.
Methods:We investigated the expression of AGAP2-AS1 in 32 CCA tissues and two CCA cell lines. We found a LncRNA AGAP2-AS1 which induced by SP1 has not been reported in CCA, and Knockdown and overexpression were used to investigate the biological role of AGAP2-AS1 in vitro. CHIP and RIP were performed to verify the putative targets of AGAP2-AS1.
Results:AGAP2-AS1 was significantly upregulated in CCA tumor tissues.SP1 induced AGAP2-AS1 plays an important role in tumorigenesis. AGAP2-AS1 knockdown significantly inhibited proliferation and caused apoptosis in CCA cells. In addition, we demonstrated that AGAP2-AS1 promotes the proliferation of CCA.
Conclusions: We conclude that the long non-coding RNA AGAP2-AS1 plays a role in promoting the proliferation of cholangiocarcinoma.
Figure 1
Figure 2
Figure 3
Figure 4