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Zhijun Wang
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Background: Continuous monocropping can affect the physicochemical and biological characteristics of cultivated soil. Sophora flavescens is a valuable herbal medicine and sensitive to continuous monocropping. Currently, diversity patterns of soil microbial communities in soil continuous monocropping with S. flavescens have not been extensively elucidated.
Results: In this study, comparative 16S rDNA and internal transcribed spacer (ITS) MiSeq sequencing analyses were used to examine the taxonomic community structure and microbial diversity in nonrhizosphere soil (CK) and rhizosphere soils (SCC, TCC, and FCC) sampled from fields that had undergone two, three, and five years of continuous monocropping, respectively. Among the microbial communities, a decreased abundance of Acidobacteria and increased abundances of Proteobacteria and Bacteroidetes were found with the increase in monocropping years of S. flavescens. As the continuous monocropping time increased, the diversity of the bacterial community decreased, but that of fungi increased. Redundancy analysis also showed that among the properties of the rhizosphere soil, the available phosphorus, organic matter, total nitrogen, and sucrase had the greatest impacts on the diversity of the rhizosphere microbial community. Moreover, a biomarker for S. flavescens soil was also identified using the most differentially abundant bacteria and fungi in soil samples.
Conclusions: Our study indicates that long-term monocropping exerted great impacts on microbial community distributions and soil physicochemical properties. The relationship between microbial community and physicochemical properties of rhizosphere soil would help clarify the side effects of continuous S. flavescens monocropping. Our study may aid in uncovering the theoretical basis underlying obstacles to continuous monocropping and provide better guidance for crop production.
Keywords
Sophora flavescens; continuous monocropping; rhizosphere soil; microbial community; soil physicochemical property
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Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- tableS1.xlsx
Table S1. The identified core OTUs of the bacterial and fungal communities.
- FigS1.pdf
Fig. S1. Top 10 bacterial (A) and fungal (B) phyla identified among the four soil samples. Each stripe denotes the mean of three replicates.
- FigS2.pdf
Fig. S2. UPGMA tree and principal component analysis of bacterial and fungal communities in soils.
- FigS3.tif
Fig. S3. LDA distribution histogram based on LEfSe analysis of classification information for bacterial and fungal communities in soils. Species with differences in LDA scores greater than 3, namely, biomarkers with significant differences, were identified [29]. The length of each bar represents the contribution of species with significant differences in abundance.
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CURRENT STATUS: Published
View the published article at BMC Microbiology.
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Posted 11 Aug, 2020
No community comments so far
Editorial decision: Accept
On 21 Aug, 2020
Editor assigned
On 06 Aug, 2020
Submission checks complete
On 05 Aug, 2020
Editor invited
On 05 Aug, 2020
No comments provided
Reviewers invited
Invitations sent on 18 Jul, 2020
Editor assigned
On 14 Jul, 2020
Submission checks complete
On 13 Jul, 2020
Editor invited
On 13 Jul, 2020
No comments provided
Editorial decision: Major revision
On 11 Jun, 2020
Review #3 received
Received 04 Jun, 2020
Review #2 received
Received 04 Jun, 2020
Review #1 received
Received 02 Jun, 2020
Reviewer #2 agreed
On 24 May, 2020
Reviewer #3 agreed
On 24 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Editor assigned
On 11 May, 2020
Submission checks complete
On 10 May, 2020
Editor invited
On 10 May, 2020
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HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
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See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Zhijun Wang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 3
Posted 11 Aug, 2020
No community comments so far
Editorial decision: Accept
On 21 Aug, 2020
Editor assigned
On 06 Aug, 2020
Submission checks complete
On 05 Aug, 2020
Editor invited
On 05 Aug, 2020
No comments provided
Reviewers invited
Invitations sent on 18 Jul, 2020
Editor assigned
On 14 Jul, 2020
Submission checks complete
On 13 Jul, 2020
Editor invited
On 13 Jul, 2020
No comments provided
Editorial decision: Major revision
On 11 Jun, 2020
Review #3 received
Received 04 Jun, 2020
Review #2 received
Received 04 Jun, 2020
Review #1 received
Received 02 Jun, 2020
Reviewer #2 agreed
On 24 May, 2020
Reviewer #3 agreed
On 24 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Editor assigned
On 11 May, 2020
Submission checks complete
On 10 May, 2020
Editor invited
On 10 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background: Continuous monocropping can affect the physicochemical and biological characteristics of cultivated soil. Sophora flavescens is a valuable herbal medicine and sensitive to continuous monocropping. Currently, diversity patterns of soil microbial communities in soil continuous monocropping with S. flavescens have not been extensively elucidated.
Results: In this study, comparative 16S rDNA and internal transcribed spacer (ITS) MiSeq sequencing analyses were used to examine the taxonomic community structure and microbial diversity in nonrhizosphere soil (CK) and rhizosphere soils (SCC, TCC, and FCC) sampled from fields that had undergone two, three, and five years of continuous monocropping, respectively. Among the microbial communities, a decreased abundance of Acidobacteria and increased abundances of Proteobacteria and Bacteroidetes were found with the increase in monocropping years of S. flavescens. As the continuous monocropping time increased, the diversity of the bacterial community decreased, but that of fungi increased. Redundancy analysis also showed that among the properties of the rhizosphere soil, the available phosphorus, organic matter, total nitrogen, and sucrase had the greatest impacts on the diversity of the rhizosphere microbial community. Moreover, a biomarker for S. flavescens soil was also identified using the most differentially abundant bacteria and fungi in soil samples.
Conclusions: Our study indicates that long-term monocropping exerted great impacts on microbial community distributions and soil physicochemical properties. The relationship between microbial community and physicochemical properties of rhizosphere soil would help clarify the side effects of continuous S. flavescens monocropping. Our study may aid in uncovering the theoretical basis underlying obstacles to continuous monocropping and provide better guidance for crop production.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- tableS1.xlsx
Table S1. The identified core OTUs of the bacterial and fungal communities.
- FigS1.pdf
Fig. S1. Top 10 bacterial (A) and fungal (B) phyla identified among the four soil samples. Each stripe denotes the mean of three replicates.
- FigS2.pdf
Fig. S2. UPGMA tree and principal component analysis of bacterial and fungal communities in soils.
- FigS3.tif
Fig. S3. LDA distribution histogram based on LEfSe analysis of classification information for bacterial and fungal communities in soils. Species with differences in LDA scores greater than 3, namely, biomarkers with significant differences, were identified [29]. The length of each bar represents the contribution of species with significant differences in abundance.
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