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Research Article
Sylwia Jafra
Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk
Corresponding Author
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Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.
Keywords
gyrB, rpoD, mrdA, cluster 17, P482, Pseudomonas donghuensis
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No competing interests reported.
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On 12 Mar, 2021
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On 10 Mar, 2021
Reviewer #12 agreed
On 10 Mar, 2021
Reviewer #13 agreed
On 10 Mar, 2021
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On 10 Mar, 2021
Reviewer #2 agreed
On 10 Mar, 2021
Reviewer #10 agreed
On 10 Mar, 2021
Reviewer #7 agreed
On 10 Mar, 2021
Reviewer #4 agreed
On 10 Mar, 2021
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Sylwia Jafra
Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, University of Gdansk
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 09 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 24 Mar, 2021
Review #2 received
Received 18 Mar, 2021
Review #3 received
Received 18 Mar, 2021
Review #4 received
Received 18 Mar, 2021
Review #1 received
Received 18 Mar, 2021
Reviewer #5 agreed
On 12 Mar, 2021
Reviewer #1 agreed
On 10 Mar, 2021
Reviewer #9 agreed
On 10 Mar, 2021
Reviewer #11 agreed
On 10 Mar, 2021
Reviewer #12 agreed
On 10 Mar, 2021
Reviewer #13 agreed
On 10 Mar, 2021
Reviewer #3 agreed
On 10 Mar, 2021
Reviewer #2 agreed
On 10 Mar, 2021
Reviewer #10 agreed
On 10 Mar, 2021
Reviewer #7 agreed
On 10 Mar, 2021
Reviewer #4 agreed
On 10 Mar, 2021
Reviewers invited
Invitations sent on 09 Mar, 2021
Editor assigned
On 08 Mar, 2021
Editor invited
On 05 Mar, 2021
Submission checks complete
On 04 Mar, 2021
First submitted
On 03 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.
Figure 1
Figure 2
Figure 3
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