Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.
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No competing interests reported.
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Posted 09 Mar, 2021
On 24 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
On 12 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
Invitations sent on 09 Mar, 2021
On 08 Mar, 2021
On 05 Mar, 2021
On 04 Mar, 2021
On 03 Mar, 2021
Posted 09 Mar, 2021
On 24 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
Received 18 Mar, 2021
On 12 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
Invitations sent on 09 Mar, 2021
On 08 Mar, 2021
On 05 Mar, 2021
On 04 Mar, 2021
On 03 Mar, 2021
Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.
Figure 1
Figure 2
Figure 3
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