This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Sundru Manjulata Devi
Bioinformatics Section, SVR BIOSCIENCE RESEARCH SERVICES, Salboni, West Bengal, India
Corresponding Author
Kumar Sharma
Division of Nephrology, Department of Medicine, University of Texas Health, Long School of Medicine, San Antonio, Texas, USA
Corresponding Author
License:
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It is widely known fact about the global pandemic caused by Severe Acute Respiratory Syndrome Coronavirus -2 (SARS-CoV-2) to humans, which imposed immediate lockdown of effected territories in the prevailing provinces. However, few provinces were able to control infection severity with lower death rates. Interestingly three types of genomic features were noticed through comparative genomics in the available genome sequences SARS-CoV-2, due to the insertion/deletions of orf3a, orf6, orf7a and orf7b. Whole genome phylogeny (n=75 genomes) revealed a large diversity within the SARS-CoV-2, and distributed in 6 clusters namely China, Diamond princess, Asian, European, USA and Beijing. This study asserts diversity in the genome with high mutation rate and migration of carriers over the world. Here, we describe the polymorphic loci of Spike glycoprotein and its putative mechanism for pathogenicity, which unveiled the presence of GPI anchor amidation, PPI hotspot, O-linked glycosylation, catalytic site, Iron binding site, signal cleavage, disulphide linkage, sulfation, transmembrane region, and C-terminal signal sites. Mutational changes at spike glycoprotein of South Korea, India, Greece, Spain, Australia, Sweden and Yunnan samples possibly suggest the prevalence of mutated strains with either low or high virulence. The regions at the spike glycoprotein also have high binding capacity to angiotensin converting enzyme 2 (ACE2) suggesting a key link for explaining damage to multiple organs including lungs, kidney and heart. Factors influencing the mutations at the spike glycoprotein region will need to be investigated to understand and neutralize the upsurge of the alarming Pandemic and to control the global spread of the disease.
Keywords
SARS-CoV-2, comparative genomics, diversity, mutational analysis, pathogenesis
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Sundru Manjulata Devi
Bioinformatics Section, SVR BIOSCIENCE RESEARCH SERVICES, Salboni, West Bengal, India
Corresponding Author
Kumar Sharma
Division of Nephrology, Department of Medicine, University of Texas Health, Long School of Medicine, San Antonio, Texas, USA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 20 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It is widely known fact about the global pandemic caused by Severe Acute Respiratory Syndrome Coronavirus -2 (SARS-CoV-2) to humans, which imposed immediate lockdown of effected territories in the prevailing provinces. However, few provinces were able to control infection severity with lower death rates. Interestingly three types of genomic features were noticed through comparative genomics in the available genome sequences SARS-CoV-2, due to the insertion/deletions of orf3a, orf6, orf7a and orf7b. Whole genome phylogeny (n=75 genomes) revealed a large diversity within the SARS-CoV-2, and distributed in 6 clusters namely China, Diamond princess, Asian, European, USA and Beijing. This study asserts diversity in the genome with high mutation rate and migration of carriers over the world. Here, we describe the polymorphic loci of Spike glycoprotein and its putative mechanism for pathogenicity, which unveiled the presence of GPI anchor amidation, PPI hotspot, O-linked glycosylation, catalytic site, Iron binding site, signal cleavage, disulphide linkage, sulfation, transmembrane region, and C-terminal signal sites. Mutational changes at spike glycoprotein of South Korea, India, Greece, Spain, Australia, Sweden and Yunnan samples possibly suggest the prevalence of mutated strains with either low or high virulence. The regions at the spike glycoprotein also have high binding capacity to angiotensin converting enzyme 2 (ACE2) suggesting a key link for explaining damage to multiple organs including lungs, kidney and heart. Factors influencing the mutations at the spike glycoprotein region will need to be investigated to understand and neutralize the upsurge of the alarming Pandemic and to control the global spread of the disease.
Figure 1
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