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Research
Guimei He
East China Normal University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Diallyl trisulfide is a component of garlic. It has been shown to have anti-fungus, anti-cancer, and anti-virus activities. We hypothesized that diallyl trisulfide can enhance host defense mechanisms against the infection by influenza virus A subtype H9N2, which is the most prevalent subtype of influenza viruses in poultry in many countries in Asia.
Methods
In this study, the effects of diallyl trisulfide on influenza virus will be investigated in vivo and in vitro respectively. The expression levels of IL-6, TNF-α, RIG-I, IRF-3, IFN-β, and H9N2 avian influenza virus M gene were detected using Quantitative real-time RT-PCR.
Results
In vitro, we found that treatment of A549 cells before or after H9N2 virus infection resulted in reduced viral loads, increased expression of antiviral genes (RIG-I, IRF-3 and interferon-β), and decreased expression of inflammatory cytokines (TNF-α and IL-6). These effects were also observed in H9N2 virus infected mice.
Conclusions
These results suggest that diallyl trisulfide inhibits H9N2 virus replication and down regulate the expression of inflammatory cytokines. Also this research indicates that diallyl trisulfide might be a kind of effective drug against influenza virus infection.
Keywords
Diallyl trisulfide, H9N2, inflammation
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Review #1 received
Received 13 Apr, 2021
Reviewer #2 agreed
On 31 Mar, 2021
Reviewer #1 agreed
On 31 Mar, 2021
Review #? received
Received 31 Mar, 2021
Reviewers invited
Invitations sent on 30 Mar, 2021
Editor assigned
On 09 Mar, 2021
Submission checks complete
On 09 Mar, 2021
Editor invited
On 09 Mar, 2021
First submitted
On 02 Mar, 2021
Metrics
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PDF Downloads: ...
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Subject Areas
Virology
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This preprint is under consideration at Virology Journal. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Guimei He
East China Normal University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Review #1 received
Received 13 Apr, 2021
Reviewer #2 agreed
On 31 Mar, 2021
Reviewer #1 agreed
On 31 Mar, 2021
Review #? received
Received 31 Mar, 2021
Reviewers invited
Invitations sent on 30 Mar, 2021
Editor assigned
On 09 Mar, 2021
Submission checks complete
On 09 Mar, 2021
Editor invited
On 09 Mar, 2021
First submitted
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Diallyl trisulfide is a component of garlic. It has been shown to have anti-fungus, anti-cancer, and anti-virus activities. We hypothesized that diallyl trisulfide can enhance host defense mechanisms against the infection by influenza virus A subtype H9N2, which is the most prevalent subtype of influenza viruses in poultry in many countries in Asia.
Methods
In this study, the effects of diallyl trisulfide on influenza virus will be investigated in vivo and in vitro respectively. The expression levels of IL-6, TNF-α, RIG-I, IRF-3, IFN-β, and H9N2 avian influenza virus M gene were detected using Quantitative real-time RT-PCR.
Results
In vitro, we found that treatment of A549 cells before or after H9N2 virus infection resulted in reduced viral loads, increased expression of antiviral genes (RIG-I, IRF-3 and interferon-β), and decreased expression of inflammatory cytokines (TNF-α and IL-6). These effects were also observed in H9N2 virus infected mice.
Conclusions
These results suggest that diallyl trisulfide inhibits H9N2 virus replication and down regulate the expression of inflammatory cytokines. Also this research indicates that diallyl trisulfide might be a kind of effective drug against influenza virus infection.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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