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Original article
Deying Ma
Corresponding Author
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The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).
Keywords
Illumina sequencing, bacterial community, cellulase, ɑ-galactosidase, L. plantarum, alfalfa silage
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CURRENT STATUS: Published
View the published article at AMB Express.
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Posted 18 Dec, 2020
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Editor assigned
On 03 Dec, 2020
Editorial decision: Accept
On 03 Dec, 2020
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On 03 Dec, 2020
Editor invited
On 03 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 24 Nov, 2020
Review #1 received
Received 15 Nov, 2020
Reviewer #1 agreed
On 12 Nov, 2020
Reviewers invited
Invitations sent on 10 Nov, 2020
Editor assigned
On 10 Oct, 2020
Submission checks complete
On 09 Oct, 2020
Editor invited
On 09 Oct, 2020
No comments provided
Editorial decision: Major Revision
On 09 Sep, 2020
Review #2 received
Received 13 Jul, 2020
Review #1 received
Received 13 Jul, 2020
Reviewer #2 agreed
On 06 Jul, 2020
Reviewer #1 agreed
On 01 Jul, 2020
Reviewers invited
Invitations sent on 27 Jun, 2020
Editor assigned
On 30 May, 2020
Editor invited
On 29 May, 2020
Submission checks complete
On 23 May, 2020
First submitted
On 22 May, 2020
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Subject Areas
Applied & Industrial Microbiology
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See the published version of this preprint at AMB Express.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Deying Ma
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at AMB Express.
Version 3
Posted 18 Dec, 2020
No community comments so far
Editor assigned
On 03 Dec, 2020
Editorial decision: Accept
On 03 Dec, 2020
Submission checks complete
On 03 Dec, 2020
Editor invited
On 03 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 24 Nov, 2020
Review #1 received
Received 15 Nov, 2020
Reviewer #1 agreed
On 12 Nov, 2020
Reviewers invited
Invitations sent on 10 Nov, 2020
Editor assigned
On 10 Oct, 2020
Submission checks complete
On 09 Oct, 2020
Editor invited
On 09 Oct, 2020
No comments provided
Editorial decision: Major Revision
On 09 Sep, 2020
Review #2 received
Received 13 Jul, 2020
Review #1 received
Received 13 Jul, 2020
Reviewer #2 agreed
On 06 Jul, 2020
Reviewer #1 agreed
On 01 Jul, 2020
Reviewers invited
Invitations sent on 27 Jun, 2020
Editor assigned
On 30 May, 2020
Editor invited
On 29 May, 2020
Submission checks complete
On 23 May, 2020
First submitted
On 22 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at AMB Express.
The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).
Figure 1
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Figure 1
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
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