This preprint is under consideration at Journal of Molecular Medicine. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research Article
Maria Calderon-Dominguez*
Biomedical Research Institute of Cadiz
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1797-3450
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing, -involving genetics, imaging, or cardiovascular techniques-, makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case-control multicentric study.
Methods: We enrolled 130 subjects: healthy controls (n=20), idiopathic DCM (n=30), ischemic DCM (n=20) and familial DCM patients which included pathogen variants of i) LMNA gene (n=30) and ii) BCL2-associated athanogene 3 (BAG3) gene (n=30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools.
Results: Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM; and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs.
Conclusions: The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker.
Keywords
Circulating circular RNA, ischemic-dilated cardiomyopathy, Lamin A/C-dilated cardiomyopathy
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Posted 18 Mar, 2021
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Internal Medicine
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This preprint is under consideration at Journal of Molecular Medicine. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Maria Calderon-Dominguez*
Biomedical Research Institute of Cadiz
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1797-3450
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Mar, 2021
No community comments so far
Reviewers invited
Invitations sent on 11 Mar, 2021
Editor assigned
On 08 Mar, 2021
First submitted
On 04 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing, -involving genetics, imaging, or cardiovascular techniques-, makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case-control multicentric study.
Methods: We enrolled 130 subjects: healthy controls (n=20), idiopathic DCM (n=30), ischemic DCM (n=20) and familial DCM patients which included pathogen variants of i) LMNA gene (n=30) and ii) BCL2-associated athanogene 3 (BAG3) gene (n=30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools.
Results: Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM; and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs.
Conclusions: The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker.
Figure 1
Figure 2
Figure 3
Figure 4
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