This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Congde Huang
Sichuan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6379-7497
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background and Aims
Human activities-mediated input of nitrogen (N) and phosphorus (P) to ecosystem may significantly affect soil hydrolyzing enzyme activities (Hy-EAs). However, the mechanisms underlying the responses of soil Hy-EAs to change in N and P availability remains unclear.
Methods
Here, a two-year field N and P addition experiment was conducted in a subtropical evergreen broad-leaved forest to elucidate the effects of N addition, P addition, and NP co-additions on soil Hy-EAs and biochemistry properties.
Results
The invertase, cellulase, and acid phosphatase activities were increased in N treatment but reduced in P treatment. The urease activity was reduced in N treatment but did not alter in P treatment. NP treatment significantly increased the invertase and cellulase activities. Furthermore, the cellulase activity was positively correlated with soil organic carbon concentration. The acid phosphatase activity was negatively correlated with microbial biomass carbon (MBC), total P, and available P concentrations. Whereas the urease activity was not strongly dependent on total N concentrations, but positively correlated with soil pH and MBC. These Hy-EAs were significantly correlated with C-to-P and N-to-P ratios, while no significantly correlation with C-to-N ratio.
Conclusions
Overall, our results indicated that N and P addition significantly affected the soil C-, N-, and P-hydrolyzing enzyme activities. With ongoing imbalanced N and P input in our studied subtropical evergreen broad-leaved forest, N addition may exacerbate the limitation of soil C and P availability, while the exogenous P addition may improve the soil C and P availability.
Keywords
N deposition, P addition, Soil hydrolyzing enzymes activities, Subtropical forest, Nutrient availability
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Congde Huang
Sichuan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6379-7497
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background and Aims
Human activities-mediated input of nitrogen (N) and phosphorus (P) to ecosystem may significantly affect soil hydrolyzing enzyme activities (Hy-EAs). However, the mechanisms underlying the responses of soil Hy-EAs to change in N and P availability remains unclear.
Methods
Here, a two-year field N and P addition experiment was conducted in a subtropical evergreen broad-leaved forest to elucidate the effects of N addition, P addition, and NP co-additions on soil Hy-EAs and biochemistry properties.
Results
The invertase, cellulase, and acid phosphatase activities were increased in N treatment but reduced in P treatment. The urease activity was reduced in N treatment but did not alter in P treatment. NP treatment significantly increased the invertase and cellulase activities. Furthermore, the cellulase activity was positively correlated with soil organic carbon concentration. The acid phosphatase activity was negatively correlated with microbial biomass carbon (MBC), total P, and available P concentrations. Whereas the urease activity was not strongly dependent on total N concentrations, but positively correlated with soil pH and MBC. These Hy-EAs were significantly correlated with C-to-P and N-to-P ratios, while no significantly correlation with C-to-N ratio.
Conclusions
Overall, our results indicated that N and P addition significantly affected the soil C-, N-, and P-hydrolyzing enzyme activities. With ongoing imbalanced N and P input in our studied subtropical evergreen broad-leaved forest, N addition may exacerbate the limitation of soil C and P availability, while the exogenous P addition may improve the soil C and P availability.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
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