The SARS-CoV-2 pandemic has challenged humankind’s ability to quickly determine the cascade of health effects caused by a novel infection. Even with the unprecedented speed at which vaccines were developed and introduced into society, identifying therapeutic interventions and drug targets for patients infected with the virus remains important as new strains of the virus may evolve, or future coronaviruses may emerge, that are resistant to current vaccines. The application of transcriptomic RNA sequencing of infected samples may shed new light on the pathways involved in viral mechanisms and host responses. We describe the application of “dual RNA-seq” analysis to consider both the host and pathogen transcriptomes simultaneously, to investigate for the first time the co-regulation of human and SARS-CoV-2 genes. Together with differential expression analysis, we describe the tissue specificity of SARS-CoV-2 expression, an inferred lipopolysaccharide response, and co-regulation of CXCL’s, SPRR’s, S100’s with SARS-CoV-2 expression. Lipopolysaccharide response pathways in particular offer promise for future therapeutic research and the prospect of subgrouping patients based on chemokine expression that may help explain the vastly different reactions patients have to infection. Taken together these findings illuminate previously unappreciated SARS-CoV-2 expression signatures, identify new therapeutic considerations, and contribute a pipeline for studying multi-transcriptome systems.
Figure 1
Figure 2
Figure 3
Figure 4
This preprint is available for download as a PDF.
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Posted 19 Mar, 2021
Received 13 Apr, 2021
Received 13 Apr, 2021
Received 13 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
Invitations sent on 30 Mar, 2021
On 30 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 04 Mar, 2021
Posted 19 Mar, 2021
Received 13 Apr, 2021
Received 13 Apr, 2021
Received 13 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
On 02 Apr, 2021
Invitations sent on 30 Mar, 2021
On 30 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 04 Mar, 2021
The SARS-CoV-2 pandemic has challenged humankind’s ability to quickly determine the cascade of health effects caused by a novel infection. Even with the unprecedented speed at which vaccines were developed and introduced into society, identifying therapeutic interventions and drug targets for patients infected with the virus remains important as new strains of the virus may evolve, or future coronaviruses may emerge, that are resistant to current vaccines. The application of transcriptomic RNA sequencing of infected samples may shed new light on the pathways involved in viral mechanisms and host responses. We describe the application of “dual RNA-seq” analysis to consider both the host and pathogen transcriptomes simultaneously, to investigate for the first time the co-regulation of human and SARS-CoV-2 genes. Together with differential expression analysis, we describe the tissue specificity of SARS-CoV-2 expression, an inferred lipopolysaccharide response, and co-regulation of CXCL’s, SPRR’s, S100’s with SARS-CoV-2 expression. Lipopolysaccharide response pathways in particular offer promise for future therapeutic research and the prospect of subgrouping patients based on chemokine expression that may help explain the vastly different reactions patients have to infection. Taken together these findings illuminate previously unappreciated SARS-CoV-2 expression signatures, identify new therapeutic considerations, and contribute a pipeline for studying multi-transcriptome systems.
Figure 1
Figure 2
Figure 3
Figure 4
This preprint is available for download as a PDF.
Loading...