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Article
Andrew Steelman
University of Illinois at Urbana-Champaign
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
The notion that myelin remains static during adulthood has been challenged in recent years. Myelin is not only crucial for proper cognitive function and behavior, but it is vulnerable to alterations from external factors outside the window of development. Here, in the adult mouse CNS, RNA analysis revealed global downregulation and subsequent recovery of oligodendrocyte-specific transcripts in response to peripheral influenza viral infection. Furthermore, shot-gun lipidomic analysis revealed that infection alters the lipid profile in the prefrontal cortex as well as in purified brain myelin. Finally, treatment with the colony stimulating factor receptor (CSFR)1 antagonist GW2580 during infection suppressed glial activation and partially restored oligodendrocyte-specific myelin transcripts to baseline levels. Together, these findings reveal a yet unforeseen consequence of peripheral infection on oligodendrocyte homeostasis in the adult mouse.
Keywords
myelin plasticity, remodeling, lipidome, oligodendrocyte, influenza, systemic infection
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This is a list of supplementary files associated with this preprint. Click to download.
- 2SupplementaryMaterialCOMBINEDFINAL.pdf
Supplementary Information
- SupplementaryMovie1.gif
Supplementary Movie 1 Animated representative 3D rendering of PLP-stained mPFC tissue of saline-inoculated mouse at day 8 p.i. Last frame of video depicts Imaris model overlaid on raw 3D data.
- SupplementaryMovie2.gif
Supplementary Movie 2 Animated representative 3D rendering of PLP-stained mPFC tissue of flu-inoculated mouse at day 8 p.i. Last frame of video depicts Imaris model overlaid on raw 3D data.
- SupplementaryMovie3.gif
Supplementary Movie 3 Animated representative 3D rendering of PLP-stained mPFC tissue of cuprizone-intoxicated mouse at 5 weeks post-cuprizone. Last frame of video depicts Imaris model overlaid on raw 3D data.
- SupplementaryData1.xlsx
Supplementary Data 1 Data output of myelin-related measures of PLP-stained mPFC tissue produced by Imaris Filament Tracer module for saline- and flu-inoculated mice at day 8 p.i. and cuprizone-intoxicated mice at 5 weeks post-cuprizone.
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This is a preprint. It has not completed peer review.
Article
Andrew Steelman
University of Illinois at Urbana-Champaign
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The notion that myelin remains static during adulthood has been challenged in recent years. Myelin is not only crucial for proper cognitive function and behavior, but it is vulnerable to alterations from external factors outside the window of development. Here, in the adult mouse CNS, RNA analysis revealed global downregulation and subsequent recovery of oligodendrocyte-specific transcripts in response to peripheral influenza viral infection. Furthermore, shot-gun lipidomic analysis revealed that infection alters the lipid profile in the prefrontal cortex as well as in purified brain myelin. Finally, treatment with the colony stimulating factor receptor (CSFR)1 antagonist GW2580 during infection suppressed glial activation and partially restored oligodendrocyte-specific myelin transcripts to baseline levels. Together, these findings reveal a yet unforeseen consequence of peripheral infection on oligodendrocyte homeostasis in the adult mouse.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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