Background: The major histocompatibility complex class I polypeptide-related sequence A gene (MICA) is one of the ligands of NKG2D activating receptor. MICA stimulates NKG2D that further triggers activation of natural killer cells which leads to killing of infected target cells. Tumor cells utilize escape strategies to subvert the biological function of NKG2D by shedding overexpressing MICA. In this study, we determine the levels of MICA colorectal cancers (CRCs). Additionally, we establish correlations between MICA expression and clinical characteristics. Publicly available data and bioinformatics tools are used for validation purposes.
Methods: We determined the MICA RNA expression levels and correlation with clinicopathological parameters in CRC using UALCAN web-portal. We performed immunohistochemical analysis on tissue microarrays having 192 samples, acquired from 96 CRC patients to validate the expression of MICA in CRC and adjacent uninvolved tissue and investigated its prognostic significance by Kaplan-Meir and proportional hazards methods.
Results: Bioinformatics and immunohistochemical analyses showed that MICA expression was significantly upregulated in CRCs as compared to uninvolved and the overexpression of MICA was independent of pathologic stage, histotype, nodal metastasis status, p53-status, as well as patient’s race, age and gender. Moreover, PROGgeneV2 survival analysis of two cohorts showed poor prognosis of CRC patients exhibiting high MICA expression.
Conclusions: Overall, our findings demonstrate high expression of MICA, suggest poor prognosis of CRC patients exhibiting high MICA expression. These results can be further explored due to its potential to provide clues to the mechanistic contributing role of the tumor microenvironment to the progression of progression of CRC.
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No competing interests reported.
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Posted 22 Mar, 2021
Invitations sent on 28 Mar, 2021
On 28 Mar, 2021
On 17 Mar, 2021
On 16 Mar, 2021
On 09 Mar, 2021
Posted 22 Mar, 2021
Invitations sent on 28 Mar, 2021
On 28 Mar, 2021
On 17 Mar, 2021
On 16 Mar, 2021
On 09 Mar, 2021
Background: The major histocompatibility complex class I polypeptide-related sequence A gene (MICA) is one of the ligands of NKG2D activating receptor. MICA stimulates NKG2D that further triggers activation of natural killer cells which leads to killing of infected target cells. Tumor cells utilize escape strategies to subvert the biological function of NKG2D by shedding overexpressing MICA. In this study, we determine the levels of MICA colorectal cancers (CRCs). Additionally, we establish correlations between MICA expression and clinical characteristics. Publicly available data and bioinformatics tools are used for validation purposes.
Methods: We determined the MICA RNA expression levels and correlation with clinicopathological parameters in CRC using UALCAN web-portal. We performed immunohistochemical analysis on tissue microarrays having 192 samples, acquired from 96 CRC patients to validate the expression of MICA in CRC and adjacent uninvolved tissue and investigated its prognostic significance by Kaplan-Meir and proportional hazards methods.
Results: Bioinformatics and immunohistochemical analyses showed that MICA expression was significantly upregulated in CRCs as compared to uninvolved and the overexpression of MICA was independent of pathologic stage, histotype, nodal metastasis status, p53-status, as well as patient’s race, age and gender. Moreover, PROGgeneV2 survival analysis of two cohorts showed poor prognosis of CRC patients exhibiting high MICA expression.
Conclusions: Overall, our findings demonstrate high expression of MICA, suggest poor prognosis of CRC patients exhibiting high MICA expression. These results can be further explored due to its potential to provide clues to the mechanistic contributing role of the tumor microenvironment to the progression of progression of CRC.
Figure 1
Figure 2
Figure 3
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