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Research article
Sri Widia A. Jusman
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8169-6825
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This work is licensed under a CC BY 4.0 License. Read Full License
Background The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria—especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein helps to regulate mitochondrial biogenesis. House geckos ( Hemidactylus platyurus ) are reptiles that have the ability to regenerate the tissue in their tails. House geckos were selected as the animal models for this study in order to analyze the association of Cygb with oxygen supply and the association of PGC-1α with energy production for tissue regeneration. Results The growth of house gecko tails showed a slow growth at the wound healing phase, then followed by a fast growth after wound healing phase of the regeneration process. While Cygb mRNA expression reached its peak at the wound healing phase and slowly decreased until the end of the observation. PGC-1α mRNA was expressed and reached its peak earlier than Cygb. Conclusions The expressions of both the Cygb and PGC-1α genes were relatively high compared to the control group. We therefore suggest that Cygb and PGC-1α play an important role during the tissue regeneration process. Keywords: cytoglobin, PGC-1α, mitochondrial biogenesis, house gecko, tissue regeneration
Keywords
cytoglobin, PGC-1α, mitochondrial biogenesis, house gecko, tissue regeneration
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CURRENT STATUS: Published
View the published article at BMC Developmental Biology.
No community comments so far
Editorial decision: Accept
On 15 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
Version 2
Posted 09 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Apr, 2020
Review #1 received
Received 08 Apr, 2020
Review #2 received
Received 29 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
No comments provided
Editorial decision: Major revision
On 07 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #2 agreed
On 02 Dec, 2019
Review #1 received
Received 10 Oct, 2019
Reviewer #1 agreed
On 27 Sep, 2019
Reviewers invited
Invitations sent on 24 Aug, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 25 Jul, 2019
Submission checks complete
On 24 Jul, 2019
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Subject Areas
Developmental Biology
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A new preprint design is coming!
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See the published version of this preprint at BMC Developmental Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Sri Widia A. Jusman
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8169-6825
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Developmental Biology.
No community comments so far
Editorial decision: Accept
On 15 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
Version 2
Posted 09 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Apr, 2020
Review #1 received
Received 08 Apr, 2020
Review #2 received
Received 29 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Reviewers invited
Invitations sent on 10 Mar, 2020
Editor assigned
On 04 Mar, 2020
Submission checks complete
On 03 Mar, 2020
Editor invited
On 03 Mar, 2020
No comments provided
Editorial decision: Major revision
On 07 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #2 agreed
On 02 Dec, 2019
Review #1 received
Received 10 Oct, 2019
Reviewer #1 agreed
On 27 Sep, 2019
Reviewers invited
Invitations sent on 24 Aug, 2019
Editor invited
On 30 Jul, 2019
Editor assigned
On 25 Jul, 2019
Submission checks complete
On 24 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Developmental Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Developmental Biology.
Background The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria—especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein helps to regulate mitochondrial biogenesis. House geckos ( Hemidactylus platyurus ) are reptiles that have the ability to regenerate the tissue in their tails. House geckos were selected as the animal models for this study in order to analyze the association of Cygb with oxygen supply and the association of PGC-1α with energy production for tissue regeneration. Results The growth of house gecko tails showed a slow growth at the wound healing phase, then followed by a fast growth after wound healing phase of the regeneration process. While Cygb mRNA expression reached its peak at the wound healing phase and slowly decreased until the end of the observation. PGC-1α mRNA was expressed and reached its peak earlier than Cygb. Conclusions The expressions of both the Cygb and PGC-1α genes were relatively high compared to the control group. We therefore suggest that Cygb and PGC-1α play an important role during the tissue regeneration process. Keywords: cytoglobin, PGC-1α, mitochondrial biogenesis, house gecko, tissue regeneration
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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