Clinicopathological features and recurrence patterns of combined hepatocellular-cholangiocarcinoma

doi:10.21203/rs.3.rs-32042/v2

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Clinicopathological features and recurrence patterns of combined hepatocellular-cholangiocarcinoma

https://doi.org/10.21203/rs.3.rs-32042/v2

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published 04 Dec, 2020

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Background: Combined hepatocellular-cholangiocarcinoma (cHCC-CCA) is a primary liver carcinoma with both hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) components. We examined the clinicopathological characteristics and recurrence patterns of cHCC-CCA. Because of the rarity of cHCC-CCA, its etiology, clinicopathological features, and prognosis in comparison with other primary liver carcinoma remain unknown. Its recurrence pattern and sites in particular also need to be elucidated.

Methods: All patients who underwent hepatectomy for primary liver malignancies between 2005 and 2015 were retrospectively included in this study.

Results: Eight hundred and ninety-four hepatectomies were performed. Nineteen cases of cHCC-CCA (2.1%) in 16 patients were enrolled. Three patients underwent re-hepatectomy. The background of hepatitis viruses and tumor marker patterns of cHCC-CCA were similar to those of HCC and dissimilar to those of intrahepatic CCA (iCCA). Biliary invasion was common in cHCC-CCA and iCCA. The 5-year overall survival values of the cHCC-CCA, HCC, and iCCA patients were 44.7%, 56.6%, and 38.5%, respectively. The 5-year recurrence-free survival values of the cHCC-CCA, HCC, and iCCA patients were 12.2%, 28.7%, and 32.9%, respectively. The liver was the most common recurrence site. Unlike HCC, however, the lymph node was the second-most common recurrence site in both cHCC-CCA and iCCA. Pathological samples of the recurrent lesions were obtained in six patients, and four had cHCC-CCA recurrence pathologically.

Conclusion: cHCC-CCA had a mixture of characteristics of HCC and iCCA. Many cases of cHCC-CCA remained cHCC-CCA pathologically even after recurrence.

General Surgery

Oncology

cHCC-CC

CHC

recurrence

Combined hepatocellular-cholangiocarcinoma (cHCC-CCA) is a relatively rare primary liver carcinoma that has both a hepatocellular carcinoma (HCC) component and cholangiocarcinoma (CCA) component in the same tumor nodule [1]. The classification of cHCC-CCA has been modified since Allen and Lisa first described it in 1949 [2], and the World Health Organization (WHO) recently updated its taxonomy [1, 3, 4]. Because of the rarity of cHCC-CCA, its etiology and clinicopathological features in comparison with other primary liver carcinoma remain unknown [5 – 11]. Although the prognosis of cHCC-CCA is often reported to be worse than that of HCC, the prognostic outcomes vary in comparison with that of iCCA [5, 8]. Its recurrence pattern and sites, the pathology of recurrent lesions and the optimal treatment for recurrence in particular need to be elucidated [12].

In the present study, we examined the clinicopathological characteristics and recurrence patterns of cHCC-CCA patients who underwent hepatectomy in a single institution. We also compared cHCC-CCA with other the primary liver carcinomas, namely HCC and intrahepatic CCA (iCCA), to clarify the biological features of cHCC-CCA.

Study design

This study was a retrospective observational study in a single institution. The study protocol was approved by the Ethics Committee of the Graduate School of Medicine, Kyoto University (R1737), and performed in accordance with the 1964 Helsinki declaration and its later amendments. Written informed consent was obtained from all study participants. All patients who received hepatectomy for primary liver malignancies between January 2005 and December 2015 were included in this study. Patients who underwent liver transplantation were excluded [13].

Peri-operative management

All patients were evaluated preoperatively using chest X-ray and contrast-enhanced computed tomography (CT) of the chest and abdomen. Additional studies including magnetic resonance imaging (MRI) and positron emission tomography were performed as needed. Hepatic resection was performed as previously described [14 – 16]. In the case of HCC, anatomical resection is basically selected. However, non-anatomical resection may be selected in cases of patients with a poor liver function. Extrahepatic bile duct resection is usually avoided. Lymph node dissection is not performed. In cases of iCCA, anatomical resection is also selected in principle. If a tumor is suspected of having invaded the hilar bile duct, extrahepatic bile duct resection is performed. Lymph node dissection is basically performed; however, it may be omitted for peripheral iCCA [17]. All patients were followed up after surgery by serum tumor markers (alpha-fetoprotein; AFP, des-gamma-carboxy prothrombin; DCP, carcinoembryonic antigen; CEA, and carbohydrate antigen 19-9; CA19-9) and contrast-enhanced CT or MRI every three to six months. Recurrence was confirmed by imaging examinations, tumor markers, and pathological examinations [14].

The pathological examination

A pathological examination was performed for all resected tumors and the background livers. The tumor size, tumor number, vascular invasion, serosal invasion, surgical margin invasion, and tumor differentiation were determined pathologically. The pathologic diagnosis was based on Hematoxylin-Eosin staining according to the WHO 2019 criteria [1], and immunohistochemical examinations for hepatocytic and cholangiocytic markers were added as needed to confirm the diagnosis.

This study defined major vascular invasion as tumor invasion to primary or secondary branches of the portal veins and/or biliary tract, and/or invasion to the main trunks of the hepatic veins or the inferior vena cava [14].

Statistical analyses

Continuous data among three groups were analyzed using Tukey’s test. Categorical data among three groups were analyzed using the chi-square test. Survival curves were estimated by the Kaplan-Meier method and compared by the log-rank test with Bonferroni’s correction. A P value of <0.05 was considered statistically significant. The JMP software package was used for all statistical analyses (JMP, Cary, NC).

Clinicopathological features

From 2005 to 2015, total of 894 cases underwent hepatectomy for primary liver tumors. Sixteen patients with cHCC-CCAs accounted for 19 cases that received hepatectomy (2.1%); 3 of the 16 cHCC-CCA patients underwent repeated hepatectomy (15.8%). Seven hundred and forty-two cases were HCCs, 121 cases were iCCAs, and 12 cases were other primary liver tumors, including malignant lymphoma, hepatic carcinosarcoma, and hepatic neuroendocrine tumor. One hundred and thirty-three of the 742 HCC patients (17.9%) and 11 of the 121 iCCA patients (9.0%) underwent repeated hepatectomy. During the same period of the study, three patients underwent living-donor liver transplantation for cHCC-CCA and were excluded from this study.

The clinicopathological features of cHCC-CCA, HCC, and iCCA are summarized in Table 1. HCC was more common in men than in women, and more patients had a worse liver function with HCC than those with iCCA. Although more than 90% patients with iCCA were negative for hepatitis B surface antigen (HBsAg), 15.8% of patients with cHCC-CCA and 19.8% of patients with HCC were positive for HBsAg. More than 80% patients with iCCA were negative for hepatitis C virus antibody (HCV-Ab), whereas more than 40% of patients with cHCC-CCA and HCC were positive for HCV-Ab. The preoperative CA19-9 levels in the iCCA patients were significantly higher than those in the cHCC-CCA or HCC patients. Patterns of viral markers and tumor markers in the cHCC-CCA patients more closely resembled those of HCC patients than those of iCCA patients. All patients with cHCC-CCA had a single cHCC-CCA tumor nodule, and 4 of the 16 cHCC-CCA patients had synchronous HCC tumor nodules. There were no significant differences in the rates of major portal vein or hepatic vein invasion among the three groups. However, the frequencies of major biliary tract invasion in cHCC-CCA and iCCA (36.8% and 18.2%, respectively) were higher than that in HCC (5.0%). At the site of the invasion into the major biliary tract of the seven cHCC-CCA patients, three had cHCC-CCA tumors, two had HCC tumors, one had an iCCA tumor, and one had an indeterminate pathology. The surgical procedures for cHCC-CCA, including the presence or absence of resection of extra-hepatic bile duct and/or lymph node dissection, were similar to those for HCC, as the preoperative diagnosis of the cHCC-CCA patients was often HCC.

Prognosis

The overall survival (OS) was compared among cHCC-CCA, HCC, and iCCA patients (Figure 1; left panel). The 5-year OS values of the cHCC-CCA, HCC, and iCCA patients were 44.7%, 56.6%, and 38.5%, respectively. The median survival times (MSTs) of the cHCC-CCA, HCC, and iCCA patients were 50.5, 72.2, and 41.7 months, respectively. The patients with iCCA had a worse prognosis than those with HCC with statistical significance (p=0.0029). The recurrence-free survival (RFS) was also compared among the three groups (Figure 1; right panel). The 5-year RFS values of the cHCC-CCA, HCC, and iCCA patients were 12.2%, 28.7%, and 32.9%, respectively. The median RFS values of the cHCC-CCA, HCC, and iCCA patients were 12.8, 21.3, and 20.3 months, respectively. There were no significant differences among the three groups.

Treatment of recurrent cHCC-CCA

Tumor recurrences and/or metastases were confirmed using imaging examinations, such as CT or MRI, and occasionally pathological examinations. The liver was the most common site of tumor recurrence among the three tumor groups (Table 2). Although the lymph nodes were the second-most common site of recurrence in both cHCC-CCA and iCCA, lymph node metastasis was less frequent in HCC. Two of the three cHCC-CCA patients with lymph node recurrences had metastases in the hepatoduodenal ligament. The treatment modalities for recurrent cHCC-CCA are summarized in Table 3. The majority of the recurrent cHCC-CCA patients were treated according to HCC recurrence.

Pathological patterns of cHCC-CCA recurrence

Pathological examinations were performed in 6 patients, and 10 samples were obtained for recurrent and/or metastatic tumors. The pathological patterns and sites of cHCC-CCA recurrence in the six patients are summarized in Table 4. Four of the patients (B, C, D, and F) had recurrent tumors of cHCC-CCA. The main component of the primary tumor in patient A was CCA, and its lymph node recurrence was CCA. However, the main component of the primary tumor in patient E was HCC, and its subsequently repeated liver recurrences were HCC.

Combined HCC-CCA is a rare primary liver carcinoma, and 2.1% of the patients who underwent surgeries for primary liver carcinoma had cHCC-CCA in our hospital, which is similar to the incidences described in previous reports [5 – 12, 18, 19]. Due to the small number of cHCC-CCA cases, it was difficult to show a statistically significant difference in a comparison among three groups. However, many patients with cHCC-CCA were male, tended to be affected by hepatitis B virus and/or hepatitis C virus, and showed high AFP and/or DCP values and normal levels of CEA and CA19-9, which resembled the clinical features of HCC but not those of iCCA. However, the cHCC-CCA patients had a higher frequency of major biliary tract invasion than the HCC patients did, showing an affinity for the biliary tract. This characteristic was similar to that of iCCA. Although there was no statistical significance, the 5-year OS and RFS rates of cHCC-CCA fell between those of HCC and iCCA. Taken together, these clinical and prognostic features suggest that cHCC-CCA has mixed characteristics of HCC and iCCA.

Pathological samples for recurrent tumors were obtained from six cHCC-CCA patients. The pathological examinations revealed that four of the six patients had cHCC-CCA in their recurrent tumors. These findings suggested that cHCC-CCA belongs to a different disease category from HCC or iCCA. However, the majority of the recurrent cHCC-CCA patients were diagnosed without pathological examinations. Therefore, a more detailed pathological evaluation on cHCC-CCA recurrence should be performed in the future.

The liver was the most common site of recurrence in cHCC-CCA, as in HCC and iCCA. However, it was similar to iCCA in that there were many lung and/or lymph node metastases in cHCC-CCA. The majority of the non-resected cases were treated using the HCC treatment protocol. This was partially because the pattern of tumor markers and the imaging findings of cHCC-CCA recurrence resemble those of HCC. The 5-year OS of cHCC-CCA was higher than that of iCCA, although the 5-year RFS of cHCC-CCA was worse than that of iCCA. This was probably because cHCC-CCA was similar to HCC and had a better response to treatment for recurrence. Due to the small number of the cHCC-CCA cases, the optimal treatment for cHCC-CCA recurrence remains unclear [20 – 22]. However, to our knowledge, this report is the first to describe the characteristics of cHCC-CCA recurrence in detail [12, 23].

It is difficult to diagnose cHCC-CCA preoperatively because there are no imaging characteristics specific to cHCC-CCA [24, 25]. In the present study, all patients with cHCC-CCA were treated as having HCC; they received hepatectomy without lymph node dissection, and a pathological examination of the surgical specimens subsequently revealed cHCC-CCA. Given that the lymph nodes were the second-most common recurrence site in cHCC-CCA, hepatectomy with lymph node dissection might be necessary if a diagnosis of cHCC-CCA is made preoperatively [23, 26].

cHCC-CCA had intermediate characteristics between HCC and iCCA in many aspects. Many cases of cHCC-CCA remained cHCC-CCA pathologically even after recurrence.

AFP: Alpha-fetoprotein

CCA: Cholangiocarcinoma

cHCC-CCA: Combined hepatocellular-cholangiocarcinoma

DCP: des-gamma carboxyprothrombin

HCC: Hepatocellular carcinoma

iCCA: Intrahepatic-cholangiocarcinoma

Acknowledgements

The authors would like to thank Japan Medical Communication (https://www.japan-mc.co.jp/about/) for English language editing.

Funding

This work was partially supported by the Grants-in-Aid for Scientific Research (19K09145).

Author information

Affiliations

Department of Surgery, Graduate School of Medicine, Kyoto University

54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Takamichi Ishii, Takashi Ito, Satoshi Ogiso, Ken Fukumitsu, Satoru Seo, Kojiro Taura, Shinji Uemoto

Department of Diagnostic Pathology, Kyoto University Hospital

54 Kawahara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Shinji Sumiyoshi

Contributions

T. Ishii was involved in the project development, data collection, data analysis, and manuscript writing. T. Ito was involved in data collection and analysis. S. Sumiyoshi was involved in pathological analysis. S.O., K.F., and S. Seo was involved in date collection. K.T. and S.U. was involved in data management. All authors have read and approved the final version of the manuscript.

Corresponding author

Correspondence to Takamichi Ishii.

Ethics declarations

Ethics approval and consent to participate

The study protocol was approved by the Ethics Committee of the Graduate School of Medicine, Kyoto University (R1721-1 and R1737), and performed in accordance with the 1964 Helsinki declaration and its later amendments.

Consent for publication

The patients provided consent for publication.

Competing interests

The authors declare that they have no competing interests.

Sempoux C, Kakar S, Kondo F, Schirmacher P. Combined hepatocellular-cholangiocarcinoma and undifferentiated primary liver carcinoma. In Digestive System Tumours. WHO Classification of Tumours, vol. 1, 5th ed. France: International Agency for Research on Cancer Press, 2019, pp 260 – 262.
Allen RA, Lisa JR Combined liver cell and bile duct carcinoma. Am J Pathol 1949:25:647-655.
Brunt E, Aishima S, Clavien PA, Fowler K, Goodman Z, et al. cHCC-CCA: Consensus terminology for primary liver carcinomas with both hepatocytic and cholangiocytic differentiation. Hepatology 2018:68:113-126.
Nagtegaal ID, Odze RD, Klimstra D, Paradis V, Rugge M, et al. The 2019 WHO classification of tumours of the digestive system. Histopathology 2020:76:182-188.
Ariizumi S, Kotera Y, Katagiri S, Nakano M, Yamamoto M Combined hepatocellular-cholangiocarcinoma had poor outcomes after hepatectomy regardless of Allen and Lisa class or the predominance of intrahepatic cholangiocarcinoma cells within the tumor. Ann Surg Oncol 2012:19:1628-1636.
Itoh S, Ikegami T, Yoshizumi T, Wang H, Takeishi K, et al. Long-term outcome of living-donor liver transplantation for combined hepatocellular-cholangiocarcinoma. Anticancer Res 2015:35:2475-2476.
Stavraka C, Rush H, Ross P Combined hepatocellular cholangiocarcinoma (cHCC-CC): an update of genetics, molecular biology, and therapeutic interventions. J Hepatocell Carcinoma 2019:6:11-21.
Wakizaka K, Yokoo H, Kamiyama T, Ohira M, Kato K, et al. Clinical and pathological features of combined hepatocellular-cholangiocarcinoma compared with other liver cancers. J Gastroenterol Hepatol 2019:34:1074-1080.
Wang AQ, Zheng YC, Du J, Zhu CP, Huang HC, et al. Combined hepatocellular cholangiocarcinoma: Controversies to be addressed. World J Gastroenterol 2016:22:4459-4465.
Wang J, Li E, Yang H, Wu J, Lu HC, et al. Combined hepatocellular-cholangiocarcinoma: a population level analysis of incidence and mortality trends. World J Surg Oncol 2019:17:43.
Yin X, Zhang BH, Qiu SJ, Ren ZG, Zhou J, et al. Combined hepatocellular carcinoma and cholangiocarcinoma: clinical features, treatment modalities, and prognosis. Ann Surg Oncol 2012:19:2869-2876.
De Vito C, Sarker D, Ross P, Heaton N, Quaglia A Histological heterogeneity in primary and metastatic classic combined hepatocellular-cholangiocarcinoma: a case series. Virchows Arch 2017:471:619-629.
Ito T, Ishii T, Sumiyoshi S, Ogiso S, Fukumitsu K, et al. Living donor liver transplantation for combined hepatocellular-cholangiocarcinoma: A case series of four patients. Int J Surg Case Rep 2020:74:46-52.
Ishii T, Hatano E, Yasuchika K, Taura K, Seo S, et al. High risk of lung metastasis after resection of hepatocellular carcinoma more than 7 cm in diameter. Surg Today 2014:44:1900-1905.
Ishii T, Fukumitsu K, Ogawa E, Okamoto T, Uemoto S Living donor liver transplantation in situs inversus totalis with a patient-specific three-dimensional printed liver model. Pediatr Transplant 2020:e13675.
Ishii T, Hatano E, Furuyama H, Manaka D, Terajima H, et al. Preventive Measures for Postoperative Bile Leakage After Central Hepatectomy: A Multicenter, Prospective, Observational Study of 101 Patients. World J Surg 2016:40:1720-1728.
Yoh T, Hatano E, Seo S, Terajima H, Uchida T, et al. Preoperative criterion identifying a low-risk group for lymph node metastasis in intrahepatic cholangiocarcinoma. J Hepatobiliary Pancreat Sci 2018:25:299-307.
Wu CH, Yong CC, Liew EH, Tsang LL, Lazo M, et al. Combined Hepatocellular Carcinoma and Cholangiocarcinoma: Diagnosis and Prognosis After Resection or Transplantation. Transplant Proc 2016:48:1100-1104.
Yu XH, Xu LB, Zeng H, Zhang R, Wang J, et al. Clinicopathological analysis of 14 patients with combined hepatocellular carcinoma and cholangiocarcinoma. Hepatobiliary Pancreat Dis Int 2011:10:620-625.
Futsukaichi Y, Tajiri K, Kobayashi S, Nagata K, Yasumura S, et al. Combined hepatocellular-cholangiocarcinoma successfully treated with sorafenib: case report and review of the literature. Clin J Gastroenterol 2019:12:128-134.
Kobayashi S, Terashima T, Shiba S, Yoshida Y, Yamada I, et al. Multicenter retrospective analysis of systemic chemotherapy for unresectable combined hepatocellular and cholangiocarcinoma. Cancer Sci 2018:109:2549-2557.
Trikalinos NA, Zhou A, Doyle MBM, Fowler KJ, Morton A, et al. Systemic Therapy for Combined Hepatocellular-Cholangiocarcinoma: A Single-Institution Experience. J Natl Compr Canc Netw 2018:16:1193-1199.
Tian MX, Luo LP, Liu WR, Deng W, Yin JC, et al. Development and validation of a prognostic score predicting recurrence in resected combined hepatocellular cholangiocarcinoma. Cancer Manag Res 2019:11:5187-5195.
Wang Y, Yang Q, Li S, Luo R, Mao S, et al. Imaging features of combined hepatocellular and cholangiocarcinoma compared with those of hepatocellular carcinoma and intrahepatic cholangiocellular carcinoma in a Chinese population. Clin Radiol 2019:74:407.e401-407.e410.
Ye J, Xie X, Lin Y, Liu B, Wang W, et al. Imaging features of combined hepatocellular-cholangiocarcinoma on contrast-enhanced ultrasound: correlation with clinicopathological findings. Clin Radiol 2018:73:237-243.
Kim KH, Lee SG, Park EH, Hwang S, Ahn CS, et al. Surgical treatments and prognoses of patients with combined hepatocellular carcinoma and cholangiocarcinoma. Ann Surg Oncol 2009:16:623-629.

Due to technical limitations, Tables 1-4 are provided in the Supplementary Files section.

Legends:

Table 1. The clinicopathological characteristics of the cHCC-CCA, HCC, and iCCA cases

HBsAg: hepatitis B surface antigen, HCV-Ab: hepatitis C virus antibody, AFP: alpha-fetoprotein, DCP: des-gamma-carboxy prothrombin, CEA: carcinoembryonic antigen: CA19-9: carbohydrate antigen 19-9, AR: anatomical resection, NAR: non-anatomical resection, NS: not significant. Numbers are described as the mean ± standard deviation. Values in parentheses are percentages.

Table 2. The recurrence sites of cHCC, HCC, and iCCA.

The values in parentheses are the percentages of all cHCC, HCC, and iCCA cases. In patients with multiple organ recurrence, each organ was counted.

Table 3. Therapeutic modalities for cHCC-CCA recurrence

TACE: transarterial chemoembolization, TAE: transarterial embolization, RFA: radiofrequency ablation, PEIT: percutaneous ethanol injection therapy, HAIC: hepatic arterial infusion chemotherapy, TKI: tyrosine-kinase inhibitor.

Table 4. The pathological patterns of cHCC-CCA recurrence in six patients

The letters in parentheses indicate the recurrent organs.

Ly: lymph node, L: liver, B: bile duct.

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Clinicopathological features and recurrence patterns of combined hepatocellular-cholangiocarcinoma

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