Cisplatin (Pt(NH4)2Cl2) and other platinum-based drugs are widely used medications that treat several different cancers, including, but not limited to, ovarian, testicular, and neck. This study presents an investigation into the correlation between the distribution of cellular platinum concentration and other elements in human glioblastoma U87 cells using the μ-PIXE technique. Cells were treated with cisplatin at concentrations of 10, 20, and 100 μM for 24 hours. The μ-PIXE technique enabled the quantification of the elements in the cells and allowed the generation of two-dimensional elemental maps with micrometric spatial resolution. By measuring the chlorine and platinum signals, we were able to identify single cells in the samples and detect those with higher platinum concentrations respectively. Moreover, we observed that the 100 μM sample had a high iron signal correlating with the platinum signal, which was not present at lower treatment concentrations. This result may indicate the induction of ferroptosis, i.e. a type of iron-dependent cell death completely independent from apoptosis.