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Research note
Jason P Chan
Marian University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8123-536X
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This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Many C. elegans aging studies use the compound, 5-fluro-2’-deoxyuridine (FUdR), to produce a synchronous population of worms in aging studies. However, it is not fully clear what effects FUdR have on the bacterial gene expression in the E. coli strain OP50, the primary laboratory C. elegans food source. This is particularly relevant as studies indicate that intestinal microbes can affect C. elegans physiology. Here, we examine how exposure to FUdR can affect gene expression changes in OP50 E. coli.
Results: RNAseq datasets were used to compare the expression patterns of E. coli genes in the strain OP50 seeded on either nematode growth media (NGM) plates or on FUdR (50µM) supplemented NGM plates. Analysis showed differential expression of genes involved in general transport, amino acid biosynthesis, transcription, iron transport, and antibiotic resistance. We specifically highlight metabolic enzymes in the L-histidine biosynthesis pathway may be regulated by FUdR exposed OP50. We conclude that OP50 exposed to FUdR results in many differentially expressed genes, including those in amino acid biosynthetic pathways.
Keywords
C. elegans, E. coli, FUdR, aging, bacterial transcriptomics
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 26 Feb, 2021
Review #1 received
Received 16 Feb, 2021
Reviewers invited
Invitations sent on 15 Feb, 2021
Reviewer #1 agreed
On 15 Feb, 2021
Editor assigned
On 10 Feb, 2021
Submission checks complete
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
First submitted
On 25 Dec, 2020
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Jason P Chan
Marian University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8123-536X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 26 Feb, 2021
Review #1 received
Received 16 Feb, 2021
Reviewers invited
Invitations sent on 15 Feb, 2021
Reviewer #1 agreed
On 15 Feb, 2021
Editor assigned
On 10 Feb, 2021
Submission checks complete
On 10 Feb, 2021
Editor invited
On 10 Feb, 2021
First submitted
On 25 Dec, 2020
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Many C. elegans aging studies use the compound, 5-fluro-2’-deoxyuridine (FUdR), to produce a synchronous population of worms in aging studies. However, it is not fully clear what effects FUdR have on the bacterial gene expression in the E. coli strain OP50, the primary laboratory C. elegans food source. This is particularly relevant as studies indicate that intestinal microbes can affect C. elegans physiology. Here, we examine how exposure to FUdR can affect gene expression changes in OP50 E. coli.
Results: RNAseq datasets were used to compare the expression patterns of E. coli genes in the strain OP50 seeded on either nematode growth media (NGM) plates or on FUdR (50µM) supplemented NGM plates. Analysis showed differential expression of genes involved in general transport, amino acid biosynthesis, transcription, iron transport, and antibiotic resistance. We specifically highlight metabolic enzymes in the L-histidine biosynthesis pathway may be regulated by FUdR exposed OP50. We conclude that OP50 exposed to FUdR results in many differentially expressed genes, including those in amino acid biosynthetic pathways.
Figure 1
Figure 2
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