Supplementary Figure 1-9
SUPPLEMENTARY FIGURE LEGENDS
Figure S1. Expression patterns of the identified marker genes in BC clusters The size of each circle depicts the percentage of cells in the cluster in which the marker was detected (≥1 UMI), and its grey scale depicts the scaled average expression level of cells within the cluster
Figure S2. Dendritic tiling of RBC1 and RBC2 BCs across the retina ocal images of retinal flat mount at outer plexiform layer level from Tg(vsx1:memCerulean)q19 694 1:memCer) and Tg(vsx2:memCerulean)wst01 695 (vsx2:memCer). Note that the vsx1:memCer line occasionally labels OFF BCs. These BCs were distinguished by tracing the cells to the axon terminals in the confocal image volumes.
Figure S3. Identification of RBC1 and RBC2 postsynaptic neurons in SBFSEM volume a, A partial image of an example SEM image of an adult zebrafish retina. OPL (outer plexiform layer), INL (inner nuclear layer), IPL (inner plexiform layer), GCL (ganglion cell layer). b, Magnified image of the region within the black box in a at the bottom layer of the IPL. Characteristic large bipolar cell axons are painted in light yellow and green. c, Ribbon synapse distributions in a RBC1 and a RBC2. The locations of ribbon synapses are marked in red. Arrow heads indicate the locations of example ribbon synapses (arrows) shown in the insets. d, Reconstruction of all RBC1s and RBC2s in the EM volume. Postsynaptic neurons of a centrally located RBC1 (open arrow head) and RBC2 (closed arrow head) were reconstructed in Fig. 5, 6, and S5-9. e,f, Traces of neuronal processes and the location of somas of cells that are post-synaptic to RBC1 and RBC2 cells. Individual cells were color coded. IPL: inner plexiform layer.
Figure S4. Ultrastructure of the zebrafish A2 AC boutons in the OFF layer Examples of large synaptic sites (arrowheads) between bi-stratifying A2-like AC and OFF BC axon terminals in the OFF layer. The A2 AC boutons often contain mitochondria (arrows).
Figure S5. Gallery of mono-stratifying AC making reciprocal synapses with RBC1 En face and side views of individual cells or processes. The numbers of input (open bar) and output (closed bar) synapses with each RBC1 (blue) and RBC2 (red) terminal are indicated as the height of the bars.
Figure S6. Gallery of mono-stratifying AC without reciprocal synapses with RBC1 En face and side views of individual cells. The numbers of input (open bar) and output (closed bar) synapses with each RBC1 (blue) and RBC2 (red) terminal are indicated as the height of the bars.
Figure S7. Gallery of bi-stratifying AC and RGC contacted to RBC1 En face and side views of individual cells. The numbers of input (open bar) and output (closed bar) synapses with each RBC1 (blue) and RBC2 (red) terminal are indicated as the height of the bars.
Figure S8. Gallery of mono-stratifying AC connected to RBC2 En face and side views of individual cells. The numbers of input (open bar) and output (closed bar) synapses with each RBC1 (blue) and RBC2 (red) terminal are indicated as the height of the bars.
Figure S9. Gallery of bi-stratifying AC and RGC connected to RBC2 En face and side views of individual cells. The numbers of input (open bar) and output (closed bar) synapses with each RBC1 (blue) and RBC2 (red) terminal are indicated as the height of the bars.