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Original research
Shenfu Injection Improves Cerebral Microcirculation and Reduces Brain Injury in a Porcine Model of Hemorrhagic Shock
Chunsheng Li
Capital Medical University Affiliated Beijing Friendship Hospital
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: The aim of this study was to clarify effects of Shenfu infusion (SFI) on cerebral microcirculation and brain injury after hemorrhagic shock (HS).
Methods: Twenty-one domestic male Beijing Landrace pigs were randomly divided into three groups: SFI group (SFI, n=8), saline group (SA, n=8) or sham operation group (SO, n=5). In the SFI group, animals were induced to HS by rapid bleeding to a mean arterial pressure of 40 mmHg within 10 minutes and maintained at 40 ± 3 mmHg for 60 minutes. Volume resuscitation (shed blood and crystalloid) and SFI were given after 1 hour of HS. In the SA group, animals received the same dose of saline instead of SFI. In the SO group, the same surgical procedure was performed but without inducing HS and volume resuscitation. The cerebral microvascular flow index (MFI), nitric oxide synthase (NOS) expression, aquaporin-4 expression, interleukin 6, tumor necrosis factor-α (TNF-α) and ultrastructural of microvascular endothelia were measured.
Results: Compared with the SA group, SFI significantly improved cerebral MFI after HS. SFI up regulated cerebral endothelial NOS expression, but down regulated interleukin 6, TNF-α, inducible NOS and aquaporin-4 expression compared with the SA group. The cerebral microvascular endothelial injury and interstitial edema in the SFI group were lighter than those in the SA group.
Conclusions: Combined application of SFI with volume resuscitation after HS can improve cerebral microcirculation and reduce brain injury.
Keywords
hemorrhagic shock, Shenfu injection, microcirculation, microvascular endothelium, brain injury
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This is a preprint. It has not completed peer review.
Original research
Shenfu Injection Improves Cerebral Microcirculation and Reduces Brain Injury in a Porcine Model of Hemorrhagic Shock
Chunsheng Li
Capital Medical University Affiliated Beijing Friendship Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Critical Care & Emergency Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The aim of this study was to clarify effects of Shenfu infusion (SFI) on cerebral microcirculation and brain injury after hemorrhagic shock (HS).
Methods: Twenty-one domestic male Beijing Landrace pigs were randomly divided into three groups: SFI group (SFI, n=8), saline group (SA, n=8) or sham operation group (SO, n=5). In the SFI group, animals were induced to HS by rapid bleeding to a mean arterial pressure of 40 mmHg within 10 minutes and maintained at 40 ± 3 mmHg for 60 minutes. Volume resuscitation (shed blood and crystalloid) and SFI were given after 1 hour of HS. In the SA group, animals received the same dose of saline instead of SFI. In the SO group, the same surgical procedure was performed but without inducing HS and volume resuscitation. The cerebral microvascular flow index (MFI), nitric oxide synthase (NOS) expression, aquaporin-4 expression, interleukin 6, tumor necrosis factor-α (TNF-α) and ultrastructural of microvascular endothelia were measured.
Results: Compared with the SA group, SFI significantly improved cerebral MFI after HS. SFI up regulated cerebral endothelial NOS expression, but down regulated interleukin 6, TNF-α, inducible NOS and aquaporin-4 expression compared with the SA group. The cerebral microvascular endothelial injury and interstitial edema in the SFI group were lighter than those in the SA group.
Conclusions: Combined application of SFI with volume resuscitation after HS can improve cerebral microcirculation and reduce brain injury.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5