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Research article
PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering
Zhenhan Deng
Shenzhen Second People's Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0522-8269
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering.
Methods
The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined.
Results
Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold.
Conclusion
The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.
Keywords
low-temperature deposition manufacturing, PLGA, collagen, hydrophilic modification, cartilage tissue engineering
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This is a preprint. It has not completed peer review.
Research article
PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering
Zhenhan Deng
Shenzhen Second People's Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0522-8269
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering.
Methods
The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined.
Results
Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold.
Conclusion
The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9