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Background
The widespread use of nanomaterials poses a great threat to human living environments. Among them, biomass cellulose nanoparticle (CN) is one of the widely used nanomaterials. To date, the toxicity of CNs during embryonic development remains undetermined. In this study, we exposed zebrafish embryos to cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs) to evaluate the toxicity of these CNs.
Results
Exposure to CNFs or CNCs below 30 mg/ml exhibited no dose-dependent increases in malformation and mortality in zebrafish embryos. Then we demonstrated that CNs were highly enriched in zebrafish embryo via imaging analysis of embryos treated with FITC-coupled CNCs. In addition, we found that CNF or CNC exposure resulted in compromised motor ability of zebrafish larva. Furthermore, it was revealed that the differentiation and the morphogenesis of motor neurons were significantly interrupted. While, blood vessels were normally patterned, suggesting the specific neurotoxicity of those materials. Transcriptome sequencing assay demonstrated that the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes.
Conclusion
We found that the specific neurotoxicity of CNF and CNC using zebrafish model. CNF or CNC exposure interrupted the morphogenesis of motor neurons, which resulted in the compromised motor ability of zebrafish larva. In addition, the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes.
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This is a preprint. It has not completed peer review.
Research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Toxicology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The widespread use of nanomaterials poses a great threat to human living environments. Among them, biomass cellulose nanoparticle (CN) is one of the widely used nanomaterials. To date, the toxicity of CNs during embryonic development remains undetermined. In this study, we exposed zebrafish embryos to cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs) to evaluate the toxicity of these CNs.
Results
Exposure to CNFs or CNCs below 30 mg/ml exhibited no dose-dependent increases in malformation and mortality in zebrafish embryos. Then we demonstrated that CNs were highly enriched in zebrafish embryo via imaging analysis of embryos treated with FITC-coupled CNCs. In addition, we found that CNF or CNC exposure resulted in compromised motor ability of zebrafish larva. Furthermore, it was revealed that the differentiation and the morphogenesis of motor neurons were significantly interrupted. While, blood vessels were normally patterned, suggesting the specific neurotoxicity of those materials. Transcriptome sequencing assay demonstrated that the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes.
Conclusion
We found that the specific neurotoxicity of CNF and CNC using zebrafish model. CNF or CNC exposure interrupted the morphogenesis of motor neurons, which resulted in the compromised motor ability of zebrafish larva. In addition, the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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