Conditions which cause hypoxia are associated with memory impairment but research models to explore the detrimental mechanisms triggered by hypoxia have been limited by the need to perform behavior testing in ambient air. Two tests of working memory, the spatial object recognition task (SOR) and spontaneous alternation in a T-maze (SA) can be minimally modified to test rodent memory within a standard hypoxia chamber to more accurately reflect conditions in which individuals suffer from impaired oxygenation throughout the task (e.g. chronic obstructive pulmonary disease, anemia, etc). Young adult normoxic cohorts perform within the chamber comparably to traditional protocols and the tests are sensitive enough to detect memory deficits under mild hypoxia (15% oxygen). These paradigms will improve the current state of knowledge regarding mechanistic pathways which are impacted during hypoxia.