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Research Article
Yu-Li Wang
Carnegie Mellon University
Corresponding Author
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Many types of adherent cells are known to reorient upon uniaxial cyclic stretching perpendicularly to the direction of stretching to facilitate such important events as wound healing, angiogenesis, and morphogenesis. While this phenomenon has been documented for decades, the underlying mechanism remains poorly understood. Using an on-stage stretching device that allowed programmable stretching with synchronized imaging, we found that the reorientation of NRK epithelial cells took place primarily during the relaxation phase when cells underwent rapid global retraction followed by extension transverse to the direction of stretching. Inhibition of myosin II caused cells to orient along the direction of stretching, whereas disassembly of microtubules enhanced transverse reorientation. Our results indicate distinct roles of stretching and relaxation in cell reorientation and implicate a role of myosin II-dependent contraction via a microtubule-modulated mechanism. The importance of relaxation phase also explains the difference between the responses to cyclic and static stretching.
Keywords
Epithelial, Cyclic Stretching, microtubule-modulated
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CURRENT STATUS: Under Review
Version 1
Posted 24 Mar, 2021
No community comments so far
Review #2 received
Received 01 Apr, 2021
Review #1 received
Received 01 Apr, 2021
Reviewer #2 agreed
On 29 Mar, 2021
Reviewer #4 agreed
On 29 Mar, 2021
Reviewer #1 agreed
On 29 Mar, 2021
Reviewer #3 agreed
On 29 Mar, 2021
Reviewers invited
Invitations sent on 27 Mar, 2021
Editor assigned
On 27 Mar, 2021
Editor invited
On 24 Mar, 2021
Submission checks complete
On 23 Mar, 2021
First submitted
On 20 Mar, 2021
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Subject Areas
Biomedical Engineering
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Yu-Li Wang
Carnegie Mellon University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Mar, 2021
No community comments so far
Review #2 received
Received 01 Apr, 2021
Review #1 received
Received 01 Apr, 2021
Reviewer #2 agreed
On 29 Mar, 2021
Reviewer #4 agreed
On 29 Mar, 2021
Reviewer #1 agreed
On 29 Mar, 2021
Reviewer #3 agreed
On 29 Mar, 2021
Reviewers invited
Invitations sent on 27 Mar, 2021
Editor assigned
On 27 Mar, 2021
Editor invited
On 24 Mar, 2021
Submission checks complete
On 23 Mar, 2021
First submitted
On 20 Mar, 2021
Subject Areas
Biomedical Engineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Many types of adherent cells are known to reorient upon uniaxial cyclic stretching perpendicularly to the direction of stretching to facilitate such important events as wound healing, angiogenesis, and morphogenesis. While this phenomenon has been documented for decades, the underlying mechanism remains poorly understood. Using an on-stage stretching device that allowed programmable stretching with synchronized imaging, we found that the reorientation of NRK epithelial cells took place primarily during the relaxation phase when cells underwent rapid global retraction followed by extension transverse to the direction of stretching. Inhibition of myosin II caused cells to orient along the direction of stretching, whereas disassembly of microtubules enhanced transverse reorientation. Our results indicate distinct roles of stretching and relaxation in cell reorientation and implicate a role of myosin II-dependent contraction via a microtubule-modulated mechanism. The importance of relaxation phase also explains the difference between the responses to cyclic and static stretching.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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