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Research article
Genome Wide Analysis and Characterization of Heat Shock Transcription Factors (Hsfs) in French Bean (Phaseolus Vulgaris L.)
Meenu kumari
Indian Institute of Horticultural Research
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0273-2873
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Heat shock transcription factors (HSFs) play an important role as transcriptional regulatory proteins against heat stress by controlling the expression of heat responsive genes. French bean is a highly thermosensitive crop and therefore, its genome sequence information segregated, characterized here in terms of heat shock transcription factors alongwith its evolutionary significance.
Results: In this study, a total comprehensive set of 29 non-redundant full length HSF genes were identified and characterized from Phaseolus vulgaris L. (PvHSF) genome sequence. Detailed gene information such as chromosomal localization, domain position, motif organization and exon- intron identification were analyzed. All the 29 PvHSF genes were mapped on 8 out of 11 chromosomes indicating the gene duplication occurred in french bean genome. Motif analysis and exon- intron structure found to be conserved in each group which showed conserved structure of PvHSFs and heat induced response is highly influenced by the cytoplasmic proteins. Based on structural features and phylogenetic relationship, the HSF genes were grouped into three classes i.e. A to C and 14 groups. Presence of only one pair of paralog sequence suggests that it may be derived from the duplication event during evolution. Comparative genomics study indicated the influence of whole genome duplication and purifying selection on french bean genome during evolution. In silico expression analysis indicated active role of classs A and B family during abiotic stress condition and higher expression in floral organs.
Conclusion: A comprehensive analysis of Heat shock transcription factors resulted 29 non-redundant full length PvHSF genes, which were characterized for their occurrence in genome and functional importance through In silico approach.
Keywords
PvHSF, heat stress, french bean, Phaseolus vulgaris, evolution
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This is a preprint. It has not completed peer review.
Research article
Genome Wide Analysis and Characterization of Heat Shock Transcription Factors (Hsfs) in French Bean (Phaseolus Vulgaris L.)
Meenu kumari
Indian Institute of Horticultural Research
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0273-2873
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Heat shock transcription factors (HSFs) play an important role as transcriptional regulatory proteins against heat stress by controlling the expression of heat responsive genes. French bean is a highly thermosensitive crop and therefore, its genome sequence information segregated, characterized here in terms of heat shock transcription factors alongwith its evolutionary significance.
Results: In this study, a total comprehensive set of 29 non-redundant full length HSF genes were identified and characterized from Phaseolus vulgaris L. (PvHSF) genome sequence. Detailed gene information such as chromosomal localization, domain position, motif organization and exon- intron identification were analyzed. All the 29 PvHSF genes were mapped on 8 out of 11 chromosomes indicating the gene duplication occurred in french bean genome. Motif analysis and exon- intron structure found to be conserved in each group which showed conserved structure of PvHSFs and heat induced response is highly influenced by the cytoplasmic proteins. Based on structural features and phylogenetic relationship, the HSF genes were grouped into three classes i.e. A to C and 14 groups. Presence of only one pair of paralog sequence suggests that it may be derived from the duplication event during evolution. Comparative genomics study indicated the influence of whole genome duplication and purifying selection on french bean genome during evolution. In silico expression analysis indicated active role of classs A and B family during abiotic stress condition and higher expression in floral organs.
Conclusion: A comprehensive analysis of Heat shock transcription factors resulted 29 non-redundant full length PvHSF genes, which were characterized for their occurrence in genome and functional importance through In silico approach.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9