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Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats
Minghan Huang
University of traditional Chinese Medicine
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Microbial Cell Factories.
In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the abundance and diversity of intestinal flora in rats. We found that the relative abundance of Firmicutes in the intestinal flora of rats with constipation was significantly higher than that in the control group, and the Bacteroides was significantly lower than that in the control group, while the relative abundance of Firmicutes was significantly changed after taking a certain dose of Chinese medicine, and reduced the relative abundance of Lachnospiraceae, and greatly increased the relative abundance of Lactobacillus, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. There was no significant difference in the total copies of intestinal bacteria between the constipated rats and the control group. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.
Keywords
Constipation, Chinese medicine, Gut microbiota, Lactobacillus, Probiotics
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CURRENT STATUS: Published
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On 18 Nov, 2020
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Review #4 received
Received 22 Jul, 2020
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On 17 Jul, 2020
Review #1 received
Received 07 Jul, 2020
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Received 02 Jul, 2020
Review #2 received
Received 02 Jul, 2020
Reviewer #2 agreed
On 01 Jul, 2020
Reviewer #3 agreed
On 01 Jul, 2020
Reviewers invited
Invitations sent on 23 Jun, 2020
Reviewer #1 agreed
On 23 Jun, 2020
Editor assigned
On 19 Jun, 2020
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On 18 Jun, 2020
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On 18 Jun, 2020
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On 15 Jun, 2020
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Subject Areas
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This preprint is under consideration at Microbial Cell Factories. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats
Minghan Huang
University of traditional Chinese Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 22 Jun, 2020
Published
On 18 Nov, 2020
No community comments so far
Review #4 received
Received 22 Jul, 2020
Reviewer #4 agreed
On 17 Jul, 2020
Review #1 received
Received 07 Jul, 2020
Review #3 received
Received 02 Jul, 2020
Review #2 received
Received 02 Jul, 2020
Reviewer #2 agreed
On 01 Jul, 2020
Reviewer #3 agreed
On 01 Jul, 2020
Reviewers invited
Invitations sent on 23 Jun, 2020
Reviewer #1 agreed
On 23 Jun, 2020
Editor assigned
On 19 Jun, 2020
Submission checks complete
On 18 Jun, 2020
Editor invited
On 18 Jun, 2020
First submitted
On 15 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Microbial Cell Factories.
In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the abundance and diversity of intestinal flora in rats. We found that the relative abundance of Firmicutes in the intestinal flora of rats with constipation was significantly higher than that in the control group, and the Bacteroides was significantly lower than that in the control group, while the relative abundance of Firmicutes was significantly changed after taking a certain dose of Chinese medicine, and reduced the relative abundance of Lachnospiraceae, and greatly increased the relative abundance of Lactobacillus, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. There was no significant difference in the total copies of intestinal bacteria between the constipated rats and the control group. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.
Figure 1
Figure 2
Figure 3
Figure 4