Background: Traditional Chinese medicine injections (TCMIs) are widely applied to treat many chronic diseases. However, product quality problems occur occasionally due to unknown constituents in TCMIs. 5-hydroxymethylfurfural (5-HMF), NƐ-(carboxymethyl)lysine (CML) and NƐ-(carboxyethyl)lysine (CEL) are three compounds generated during food and Chinese medicinal herb processing and may be harmful to human health.
Methods: In this study, the contents of 5-HMF, CML and CEL were determined by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). For 5-HMF, the separation was performed on a Hypersil ODS2 column (250 mm×4.6 mm, 5 µm), and the column temperature was set at 30℃. The mobile phase was composed of water-methanol (95:5) at a flow rate of 1.0 mL/min. For CML and CEL, separation was performed on a CORTECS HILIC UPLC column (2.1 mm×50 mm, 1.6 µm), and the column temperature was set at 40℃. The mobile phase was composed of acetonitrile-water (3:7) at a flow rate of 0.3 mL/min. Multiple-reaction monitoring mode was employed for analyte determination with positive ionization.
Results: The contents of 5-HMF in 16 TCMIs varied from 0.19 to 74.98 µg/mL, with a larger variation than the contents of CML and CEL. The Ciwujia injection had the highest content of 5-HMF, and the Qingkailing injection had the lowest 5-HMF content. The contents of CML and CEL among these TCMIs were 0.51-7.32 ng·mL-1 and 0.38-5.49 ng·mL-1, respectively. The contents of CML and CEL in the Shuxuetong injection were much higher than in the others.
Conclusions: The methods established in this study were simple, rapid and accurate and could provide a theoretical basis for the quality evaluation of TCMIs.
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Posted 18 Jun, 2020
On 17 Jun, 2020
On 16 Jun, 2020
On 16 Jun, 2020
On 16 Jun, 2020
Posted 18 Jun, 2020
On 17 Jun, 2020
On 16 Jun, 2020
On 16 Jun, 2020
On 16 Jun, 2020
Background: Traditional Chinese medicine injections (TCMIs) are widely applied to treat many chronic diseases. However, product quality problems occur occasionally due to unknown constituents in TCMIs. 5-hydroxymethylfurfural (5-HMF), NƐ-(carboxymethyl)lysine (CML) and NƐ-(carboxyethyl)lysine (CEL) are three compounds generated during food and Chinese medicinal herb processing and may be harmful to human health.
Methods: In this study, the contents of 5-HMF, CML and CEL were determined by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). For 5-HMF, the separation was performed on a Hypersil ODS2 column (250 mm×4.6 mm, 5 µm), and the column temperature was set at 30℃. The mobile phase was composed of water-methanol (95:5) at a flow rate of 1.0 mL/min. For CML and CEL, separation was performed on a CORTECS HILIC UPLC column (2.1 mm×50 mm, 1.6 µm), and the column temperature was set at 40℃. The mobile phase was composed of acetonitrile-water (3:7) at a flow rate of 0.3 mL/min. Multiple-reaction monitoring mode was employed for analyte determination with positive ionization.
Results: The contents of 5-HMF in 16 TCMIs varied from 0.19 to 74.98 µg/mL, with a larger variation than the contents of CML and CEL. The Ciwujia injection had the highest content of 5-HMF, and the Qingkailing injection had the lowest 5-HMF content. The contents of CML and CEL among these TCMIs were 0.51-7.32 ng·mL-1 and 0.38-5.49 ng·mL-1, respectively. The contents of CML and CEL in the Shuxuetong injection were much higher than in the others.
Conclusions: The methods established in this study were simple, rapid and accurate and could provide a theoretical basis for the quality evaluation of TCMIs.
Figure 1
Figure 2
Figure 3
Figure 4
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