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Determination of 5-Hydroxymethylfurfural, NƐ-(Carboxymethyl)Lysine and NƐ-(Carboxyethyl)Lysine in 16 Traditional Chinese Medicine Injections Based on HPLC and UPLC-MS/MS
Yinshi Sun
Chinese Academy of Agricultural Sciences Institute of Special Animal and Plant Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1889-4984
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Traditional Chinese medicine injections (TCMIs) are widely applied to treat many chronic diseases. However, product quality problems occur occasionally due to unknown constituents in TCMIs. 5-hydroxymethylfurfural (5-HMF), NƐ-(carboxymethyl)lysine (CML) and NƐ-(carboxyethyl)lysine (CEL) are three compounds generated during food and Chinese medicinal herb processing and may be harmful to human health.
Methods: In this study, the contents of 5-HMF, CML and CEL were determined by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). For 5-HMF, the separation was performed on a Hypersil ODS2 column (250 mm×4.6 mm, 5 µm), and the column temperature was set at 30℃. The mobile phase was composed of water-methanol (95:5) at a flow rate of 1.0 mL/min. For CML and CEL, separation was performed on a CORTECS HILIC UPLC column (2.1 mm×50 mm, 1.6 µm), and the column temperature was set at 40℃. The mobile phase was composed of acetonitrile-water (3:7) at a flow rate of 0.3 mL/min. Multiple-reaction monitoring mode was employed for analyte determination with positive ionization.
Results: The contents of 5-HMF in 16 TCMIs varied from 0.19 to 74.98 µg/mL, with a larger variation than the contents of CML and CEL. The Ciwujia injection had the highest content of 5-HMF, and the Qingkailing injection had the lowest 5-HMF content. The contents of CML and CEL among these TCMIs were 0.51-7.32 ng·mL-1 and 0.38-5.49 ng·mL-1, respectively. The contents of CML and CEL in the Shuxuetong injection were much higher than in the others.
Conclusions: The methods established in this study were simple, rapid and accurate and could provide a theoretical basis for the quality evaluation of TCMIs.
Keywords
Traditional Chinese medicine injections, 5-HMF, CML, CEL, HPLC, UPLC-MS/MS
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This preprint is under consideration at Chinese Medicine. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Determination of 5-Hydroxymethylfurfural, NƐ-(Carboxymethyl)Lysine and NƐ-(Carboxyethyl)Lysine in 16 Traditional Chinese Medicine Injections Based on HPLC and UPLC-MS/MS
Yinshi Sun
Chinese Academy of Agricultural Sciences Institute of Special Animal and Plant Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1889-4984
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Jun, 2020
No community comments so far
Editor assigned
On 17 Jun, 2020
First submitted
On 16 Jun, 2020
Submission checks complete
On 16 Jun, 2020
Editor invited
On 16 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Hospital Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Traditional Chinese medicine injections (TCMIs) are widely applied to treat many chronic diseases. However, product quality problems occur occasionally due to unknown constituents in TCMIs. 5-hydroxymethylfurfural (5-HMF), NƐ-(carboxymethyl)lysine (CML) and NƐ-(carboxyethyl)lysine (CEL) are three compounds generated during food and Chinese medicinal herb processing and may be harmful to human health.
Methods: In this study, the contents of 5-HMF, CML and CEL were determined by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). For 5-HMF, the separation was performed on a Hypersil ODS2 column (250 mm×4.6 mm, 5 µm), and the column temperature was set at 30℃. The mobile phase was composed of water-methanol (95:5) at a flow rate of 1.0 mL/min. For CML and CEL, separation was performed on a CORTECS HILIC UPLC column (2.1 mm×50 mm, 1.6 µm), and the column temperature was set at 40℃. The mobile phase was composed of acetonitrile-water (3:7) at a flow rate of 0.3 mL/min. Multiple-reaction monitoring mode was employed for analyte determination with positive ionization.
Results: The contents of 5-HMF in 16 TCMIs varied from 0.19 to 74.98 µg/mL, with a larger variation than the contents of CML and CEL. The Ciwujia injection had the highest content of 5-HMF, and the Qingkailing injection had the lowest 5-HMF content. The contents of CML and CEL among these TCMIs were 0.51-7.32 ng·mL-1 and 0.38-5.49 ng·mL-1, respectively. The contents of CML and CEL in the Shuxuetong injection were much higher than in the others.
Conclusions: The methods established in this study were simple, rapid and accurate and could provide a theoretical basis for the quality evaluation of TCMIs.
Figure 1
Figure 2
Figure 3
Figure 4