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Research Article
Murugadas Vaiyapuri
CIFT: Central Institute of Fisheries Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5807-9290
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Antimicrobial resistance (AMR) burden in Escherichia coli along the 90 km stretch of Vembanad Lake, Kerala, India was assessed. Seventy-seven percent of water samples drawn from 35 different stations of the Lake harbored E. coli. Antibiotic susceptibility test performed on 116 E. coli isolates revealed 81% were resistant to ≥ one antibiotic with 39 AMR profiles, 30% multidrug resistant, 32% extended spectrum b lactamase (ESBL) producers as per CLSI.The probability of isolating cefotaxime resistant E. coli was the highest 0.7 (P ≤ 0.05) in the Lake. Genetically diverse ESBL types blaTEM-116,blaCTX-M -152, blaCTX-M -27,blaCTX-M -55, blaCTX-M-205, and blaSHV-27 were identified. Molecular typing (ERIC PCR, MLST and PBRT) confirmed the diversity among E. coli between and within the stations. ST11439 and Single and Double Loci Variants of ST443, ST4533 were identified in Multi locus sequence typing (MLST) analysis. Inc plasmids (B/O, F, W, I1, FIIA, HI1, P-1a, K/B and N) identified in the Lake evidences the transmission potential. Low multiple antibiotic resistance index (average < 0.2) indicating lower risk to the human population albeit, an emerging concern of ESBL resistance in the Lake. The occurrence of genetically variant ESBL E. coli in Vembanad Lake signals health hazards and necessitates pragmatizing strategic control measures.
Keywords
Vembanad Lake, AntimicrobialResistance, Extended Spectrum Beta LactamaseEscherichia coli, Water, Moleculartyping
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This is a list of supplementary files associated with this preprint. Click to download.
- Table.S12021.docx
Supplementary file Resistance mapping and genetic diversity of ESBLEscherichia coli isolated from largest fresh cum brackish water of the Vembanad Lake, Kerala, India Appendix A. Supplementary data The base data generated in this study is attached as a supplementary file as Table S1.
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View the published article at Environmental Science and Pollution Research.
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Editorial decision: Major Revision
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See the published version of this preprint at Environmental Science and Pollution Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Murugadas Vaiyapuri
CIFT: Central Institute of Fisheries Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5807-9290
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Environmental Science and Pollution Research.
Version 1
Posted 19 Apr, 2021
No community comments so far
Editorial decision: Major Revision
On 18 May, 2021
Reviewers invited
Invitations sent on 11 Apr, 2021
Editor assigned
On 28 Mar, 2021
First submitted
On 25 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Environmental Science and Pollution Research.
Antimicrobial resistance (AMR) burden in Escherichia coli along the 90 km stretch of Vembanad Lake, Kerala, India was assessed. Seventy-seven percent of water samples drawn from 35 different stations of the Lake harbored E. coli. Antibiotic susceptibility test performed on 116 E. coli isolates revealed 81% were resistant to ≥ one antibiotic with 39 AMR profiles, 30% multidrug resistant, 32% extended spectrum b lactamase (ESBL) producers as per CLSI.The probability of isolating cefotaxime resistant E. coli was the highest 0.7 (P ≤ 0.05) in the Lake. Genetically diverse ESBL types blaTEM-116,blaCTX-M -152, blaCTX-M -27,blaCTX-M -55, blaCTX-M-205, and blaSHV-27 were identified. Molecular typing (ERIC PCR, MLST and PBRT) confirmed the diversity among E. coli between and within the stations. ST11439 and Single and Double Loci Variants of ST443, ST4533 were identified in Multi locus sequence typing (MLST) analysis. Inc plasmids (B/O, F, W, I1, FIIA, HI1, P-1a, K/B and N) identified in the Lake evidences the transmission potential. Low multiple antibiotic resistance index (average < 0.2) indicating lower risk to the human population albeit, an emerging concern of ESBL resistance in the Lake. The occurrence of genetically variant ESBL E. coli in Vembanad Lake signals health hazards and necessitates pragmatizing strategic control measures.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Table.S12021.docx
Supplementary file Resistance mapping and genetic diversity of ESBLEscherichia coli isolated from largest fresh cum brackish water of the Vembanad Lake, Kerala, India Appendix A. Supplementary data The base data generated in this study is attached as a supplementary file as Table S1.
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