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Accuracy of HPV E6/E7 RNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
Hui Chang
Weifang Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Diagnostic Pathology.
Background: The consistency of pathologists in the diagnosis of cervical intraepithelial neoplasia (CINs) is not ideal, especially between low- and high-grade squamous intraepithelial lision (LSIL and HSIL). This study was aimed to explore efficient strategies for the grading diagnosis of CINs.
Methods: The medical records of patients with high risk HPV (HR-HPV) infections who had undergone cervical biopsy or conization from April 2018 to April 2019 in Beijing Chao-Yang Hospital were collected and examined. HR-HPV E6/E7mRNA in tissues of patients with CINs was detected using RNAscope chromogenic in situ hybridization (RISH). Immunohistochemistry (IHC) was performed to evelute the expression of p16INK4a (P16) and Ki67.
Results: HR-HPV E6/E7 mRNA signals were detected in 3/27(11.1%) CIN 1, 32/33(97.0%) CIN 2/3. Mostly staining patterns(27/32, 84.4%) showed full-thickness epithelial layer staining with weak to strong nuclear and cytoplasmic dot-like signals in CIN 2/3, there were also a few special staining patterns significant differences from others. We compared a number of indicators in LISL and HSIL differences. There were statistically significant differences in E6/E7mRNA, p16, Ki67 and cytology between the two groups (P<0.05). According to logistic regression analysis，there was only E6/E7mRNA positivity was significantly associated with CIN2/3(OR: 52.53, 95% CI, P<0.05). In the detection of CIN2/3, The sensitivity and specificity of HPV E6/E7mRNA alone were not sinificantly inferior to that its different combinations with Ki67, p16, and cytology (all P >0.05).
Conclusions: RISH was efficient in grading diagnosis of CINs. HPV E6/E7mRNA expressions might reflet the phase HPV infections and its positive pattern might predict the development direction of CINs, which could provide the possibility to realize more accurate treatment for patients.
Keywords
cervical intraepithelial neoplasia, CIN, diagnostic accuracy, E6/E7mRNA, in situ hybridization
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This preprint is under consideration at Diagnostic Pathology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Accuracy of HPV E6/E7 RNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
Hui Chang
Weifang Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 22 Jun, 2020
Published
On 19 Feb, 2021
No community comments so far
Editorial decision: Revise before peer review
On 28 Jun, 2020
Editor assigned
On 25 Jun, 2020
Editor invited
On 24 Jun, 2020
Submission checks complete
On 19 Jun, 2020
First submitted
On 18 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Pathology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Diagnostic Pathology.
Background: The consistency of pathologists in the diagnosis of cervical intraepithelial neoplasia (CINs) is not ideal, especially between low- and high-grade squamous intraepithelial lision (LSIL and HSIL). This study was aimed to explore efficient strategies for the grading diagnosis of CINs.
Methods: The medical records of patients with high risk HPV (HR-HPV) infections who had undergone cervical biopsy or conization from April 2018 to April 2019 in Beijing Chao-Yang Hospital were collected and examined. HR-HPV E6/E7mRNA in tissues of patients with CINs was detected using RNAscope chromogenic in situ hybridization (RISH). Immunohistochemistry (IHC) was performed to evelute the expression of p16INK4a (P16) and Ki67.
Results: HR-HPV E6/E7 mRNA signals were detected in 3/27(11.1%) CIN 1, 32/33(97.0%) CIN 2/3. Mostly staining patterns(27/32, 84.4%) showed full-thickness epithelial layer staining with weak to strong nuclear and cytoplasmic dot-like signals in CIN 2/3, there were also a few special staining patterns significant differences from others. We compared a number of indicators in LISL and HSIL differences. There were statistically significant differences in E6/E7mRNA, p16, Ki67 and cytology between the two groups (P<0.05). According to logistic regression analysis，there was only E6/E7mRNA positivity was significantly associated with CIN2/3(OR: 52.53, 95% CI, P<0.05). In the detection of CIN2/3, The sensitivity and specificity of HPV E6/E7mRNA alone were not sinificantly inferior to that its different combinations with Ki67, p16, and cytology (all P >0.05).
Conclusions: RISH was efficient in grading diagnosis of CINs. HPV E6/E7mRNA expressions might reflet the phase HPV infections and its positive pattern might predict the development direction of CINs, which could provide the possibility to realize more accurate treatment for patients.
Figure 1
Figure 2