Accuracy of HPV E6/E7 RNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
Background: The consistency of pathologists in the diagnosis of cervical intraepithelial neoplasia (CINs) is not ideal, especially between low- and high-grade squamous intraepithelial lision (LSIL and HSIL). This study was aimed to explore efficient strategies for the grading diagnosis of CINs.
Methods: The medical records of patients with high risk HPV (HR-HPV) infections who had undergone cervical biopsy or conization from April 2018 to April 2019 in Beijing Chao-Yang Hospital were collected and examined. HR-HPV E6/E7mRNA in tissues of patients with CINs was detected using RNAscope chromogenic in situ hybridization (RISH). Immunohistochemistry (IHC) was performed to evelute the expression of p16INK4a (P16) and Ki67.
Results: HR-HPV E6/E7 mRNA signals were detected in 3/27(11.1%) CIN 1, 32/33(97.0%) CIN 2/3. Mostly staining patterns(27/32, 84.4%) showed full-thickness epithelial layer staining with weak to strong nuclear and cytoplasmic dot-like signals in CIN 2/3, there were also a few special staining patterns significant differences from others. We compared a number of indicators in LISL and HSIL differences. There were statistically significant differences in E6/E7mRNA, p16, Ki67 and cytology between the two groups (P<0.05). According to logistic regression analysis,there was only E6/E7mRNA positivity was significantly associated with CIN2/3(OR: 52.53, 95% CI, P<0.05). In the detection of CIN2/3, The sensitivity and specificity of HPV E6/E7mRNA alone were not sinificantly inferior to that its different combinations with Ki67, p16, and cytology (all P >0.05).
Conclusions: RISH was efficient in grading diagnosis of CINs. HPV E6/E7mRNA expressions might reflet the phase HPV infections and its positive pattern might predict the development direction of CINs, which could provide the possibility to realize more accurate treatment for patients.
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Posted 22 Jun, 2020
On 19 Feb, 2021
On 28 Jun, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 19 Jun, 2020
On 18 Jun, 2020
Accuracy of HPV E6/E7 RNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
Posted 22 Jun, 2020
On 19 Feb, 2021
On 28 Jun, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 19 Jun, 2020
On 18 Jun, 2020
Background: The consistency of pathologists in the diagnosis of cervical intraepithelial neoplasia (CINs) is not ideal, especially between low- and high-grade squamous intraepithelial lision (LSIL and HSIL). This study was aimed to explore efficient strategies for the grading diagnosis of CINs.
Methods: The medical records of patients with high risk HPV (HR-HPV) infections who had undergone cervical biopsy or conization from April 2018 to April 2019 in Beijing Chao-Yang Hospital were collected and examined. HR-HPV E6/E7mRNA in tissues of patients with CINs was detected using RNAscope chromogenic in situ hybridization (RISH). Immunohistochemistry (IHC) was performed to evelute the expression of p16INK4a (P16) and Ki67.
Results: HR-HPV E6/E7 mRNA signals were detected in 3/27(11.1%) CIN 1, 32/33(97.0%) CIN 2/3. Mostly staining patterns(27/32, 84.4%) showed full-thickness epithelial layer staining with weak to strong nuclear and cytoplasmic dot-like signals in CIN 2/3, there were also a few special staining patterns significant differences from others. We compared a number of indicators in LISL and HSIL differences. There were statistically significant differences in E6/E7mRNA, p16, Ki67 and cytology between the two groups (P<0.05). According to logistic regression analysis,there was only E6/E7mRNA positivity was significantly associated with CIN2/3(OR: 52.53, 95% CI, P<0.05). In the detection of CIN2/3, The sensitivity and specificity of HPV E6/E7mRNA alone were not sinificantly inferior to that its different combinations with Ki67, p16, and cytology (all P >0.05).
Conclusions: RISH was efficient in grading diagnosis of CINs. HPV E6/E7mRNA expressions might reflet the phase HPV infections and its positive pattern might predict the development direction of CINs, which could provide the possibility to realize more accurate treatment for patients.
Figure 1
Figure 2