This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Yingping Xiao
Zhejiang Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7949-1126
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This work is licensed under a CC BY 4.0 License. Read Full License
Background:During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serumof geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach.
Results:Sixty Landes geese of the same age were randomly divided into the following 3 groups: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding.Gas chromatography time-of-flight/massspectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlatedmetabolic pathways. A large number of endogenous molecules in serum were altered, especially at the late stage of overfeeding (7 days to 25 days). Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in various amino acids metabolism, carbohydrate metabolismand lipid metabolism.
Conclusions: These findings pinpoint specific metabolite changes and identify potential biomarkers for early diagnosis of fatty liver disease, as well as provide insights into the perturbation of metabolic pathways involved in fatty liver formation.
Keywords
Overfeeding,Fatty liver, Serum metabolomic, Serum enzymatic activities, Serum lipid levels,Landes geese
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Yingping Xiao
Zhejiang Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7949-1126
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 24 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background:During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serumof geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach.
Results:Sixty Landes geese of the same age were randomly divided into the following 3 groups: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding.Gas chromatography time-of-flight/massspectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlatedmetabolic pathways. A large number of endogenous molecules in serum were altered, especially at the late stage of overfeeding (7 days to 25 days). Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in various amino acids metabolism, carbohydrate metabolismand lipid metabolism.
Conclusions: These findings pinpoint specific metabolite changes and identify potential biomarkers for early diagnosis of fatty liver disease, as well as provide insights into the perturbation of metabolic pathways involved in fatty liver formation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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